Gene ontology annotations for HLA-DRB5
Experiment description of studies that identified HLA-DRB5 in exosomes
1
Experiment ID
11
MISEV standards
✘
EV Biophysical techniques
✔
HSC70|HSP90|MHCII|CD63|CD81
EV Enriched markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry [MALDI TOF] Western blotting
PubMed ID
12519789
Organism
Homo sapiens
Experiment description
Proteomic and biochemical analyses of human B cell-derived exosomes. Potential implications for their function and multivesicular body formation.
Authors
Wubbolts R, Leckie RS, Veenhuizen PT, Schwarzmann G, Mobius W, Hoernschemeyer J, Slot JW, Geuze HJ, Stoorvogel W
Journal name
JBC
Publication year
2003
Sample
B cells
Sample name
RN (HLA-DR15+)
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
1.15 g/mL
Molecules identified in the study
Protein Lipids
Methods used in the study
Mass spectrometry [MALDI TOF] Mass spectrometry [QTOF] Western blotting Thin layer chromatography HPLC
2
Experiment ID
16
MISEV standards
✔
EM
EV Biophysical techniques
✔
MHCII|CD86|LAMP1|LAMP2|CD63|CD81|CD82|ICAM1|TFRC
EV Enriched markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting Immunoelectron Microscopy
PubMed ID
9685355
Organism
Homo sapiens
Experiment description
Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes.
Authors
Escola JM, Kleijmeer MJ, Stoorvogel W, Griffith JM, Yoshie O, Geuze HJ
Journal name
JBC
Publication year
1998
Sample
B cells
Sample name
RN (HLA-DR15+)
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
1.12-1.18 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Immunoelectron Microscopy
3
Experiment ID
79
MISEV standards
✘
EV Biophysical techniques
✔
CD81|MHCII
EV Enriched markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
20458337
Organism
Homo sapiens
Experiment description
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name
ICB
Publication year
2010
Sample
B cells
Sample name
RN (HLA-DR15)
Isolation/purification methods
Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II)
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [FT-ICR] Western blotting
4
Experiment ID
80
MISEV standards
✘
EV Biophysical techniques
✔
CD81|MHCII
EV Enriched markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
20458337
Organism
Homo sapiens
Experiment description
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name
ICB
Publication year
2010
Sample
B cells
Sample name
RN (HLA-DR15)
Isolation/purification methods
Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II)
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [FT-ICR] Western blotting
5
Experiment ID
81
MISEV standards
✘
EV Biophysical techniques
✔
CD81|MHCII
EV Enriched markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
20458337
Organism
Homo sapiens
Experiment description
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name
ICB
Publication year
2010
Sample
B cells
Sample name
RN (HLA-DR15)
Isolation/purification methods
Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II)
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [FT-ICR] Western blotting
6
Experiment ID
124
MISEV standards
✔
IEM|EM
EV Biophysical techniques
✔
HSC70|CD63|CD9|CD81
EV Enriched markers
✔
CANX
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
FACS
PubMed ID
21235781
Organism
Homo sapiens
Experiment description
Human saliva, plasma and breast milk exosomes contain RNA: uptake by macrophages.
Authors
Lasser C, Alikhani VS, Ekstrom K, Eldh M, Paredes PT, Bossios A, Sjostrand M, Gabrielsson S, Lotvall J, Valadi H.
Journal name
JTM
Publication year
2011
Sample
Breast milk
Sample name
Breast milk - Normal
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
FACS Immunoelectron Microscopy Western blotting
7
Experiment ID
363
MISEV standards
✘
EV Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
✔
DCLK1
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
33991177
Organism
Homo sapiens
Experiment description
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors
Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name
Proteomics
Publication year
2021
Sample
Gastric cancer cells
Sample name
MKN1 - 100K pellet
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
8
Experiment ID
364
MISEV standards
✘
EV Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
✔
DCLK1
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
33991177
Organism
Homo sapiens
Experiment description
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors
Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name
Proteomics
Publication year
2021
Sample
Gastric cancer cells
Sample name
MKN1 - 100K pellet
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
9
Experiment ID
365
MISEV standards
✘
EV Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
✔
DCLK1
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
33991177
Organism
Homo sapiens
Experiment description
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors
Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name
Proteomics
Publication year
2021
Sample
Gastric cancer cells
Sample name
MKN1 - 100K pellet
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
10
Experiment ID
123
MISEV standards
✔
IEM|EM
EV Biophysical techniques
✔
CD63|CD9|CD81
EV Enriched markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
FACS
PubMed ID
21235781
Organism
Homo sapiens
Experiment description
Human saliva, plasma and breast milk exosomes contain RNA: uptake by macrophages.
Authors
Lasser C, Alikhani VS, Ekstrom K, Eldh M, Paredes PT, Bossios A, Sjostrand M, Gabrielsson S, Lotvall J, Valadi H.
Journal name
JTM
Publication year
2011
Sample
Plasma
Sample name
Plasma - Normal
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
FACS Immunoelectron Microscopy Western blotting
11
Experiment ID
231
MISEV standards
✘
EV Biophysical techniques
✔
Alix|CD63|CD9
EV Enriched markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein Lipids
Methods used in the study
Western blotting Mass spectrometry
12
Experiment ID
232
MISEV standards
✘
EV Biophysical techniques
✘
EV Enriched markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 2
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
13
Experiment ID
233
MISEV standards
✘
EV Biophysical techniques
✘
EV Enriched markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 3
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
14
Experiment ID
122
MISEV standards
✔
IEM|EM
EV Biophysical techniques
✔
CD63|CD9|CD81
EV Enriched markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
FACS
PubMed ID
21235781
Organism
Homo sapiens
Experiment description
Human saliva, plasma and breast milk exosomes contain RNA: uptake by macrophages.
Authors
Lasser C, Alikhani VS, Ekstrom K, Eldh M, Paredes PT, Bossios A, Sjostrand M, Gabrielsson S, Lotvall J, Valadi H.
Journal name
JTM
Publication year
2011
Sample
Saliva
Sample name
Saliva - Normal
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
FACS Immunoelectron Microscopy Western blotting
15
Experiment ID
18
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63
EV Enriched markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
FACS
PubMed ID
11907077
Organism
Homo sapiens
Experiment description
TCR activation of human T cells induces the production of exosomes bearing the TCR/CD3/zeta complex.
Authors
Blanchard N, Lankar D, Faure F, Regnault A, Dumont C, Raposo G, Hivroz C
Journal name
JIMMU
Publication year
2002
Sample
T cells
Sample name
Jurkat T cell blasts
Isolation/purification methods
Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
FACS
16
Experiment ID
217
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|CD81|CD9|CD63
EV Enriched markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23844026
Organism
Homo sapiens
Experiment description
Characterization of human thymic exosomes.
Authors
Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name
PLoS One
Publication year
2013
Sample
Thymus
Sample name
Normal-Thymus
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
Protein-protein interactions for HLA-DRB5
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
HLA-DRA
3122
Invitro
Homo sapiens
Affinity Capture-MS
Homo sapiens
View the network
image/svg+xml
Pathways in which HLA-DRB5 is involved