Gene description for HLA-DRB5
Gene name major histocompatibility complex, class II, DR beta 5
Gene symbol HLA-DRB5
Other names/aliases HLA-DRB
Species Homo sapiens
 Database cross references - HLA-DRB5
ExoCarta ExoCarta_3127
Entrez Gene 3127
HGNC 4953
MIM 604776
UniProt Q30154  
 HLA-DRB5 identified in exosomes derived from the following tissue/cell type
B cells 12519789    
B cells 9685355    
B cells 20458337    
B cells 20458337    
B cells 20458337    
Breast milk 21235781    
Plasma 21235781    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Saliva 21235781    
T cells 11907077    
Thymus 23844026    
 Gene ontology annotations for HLA-DRB5
Molecular Function
    peptide antigen binding GO:0042605 ISS
Biological Process
    T cell receptor signaling pathway GO:0050852 TAS
    negative regulation of T cell proliferation GO:0042130 ISS
    immunoglobulin production involved in immunoglobulin mediated immune response GO:0002381 ISS
    T cell costimulation GO:0031295 TAS
    antigen processing and presentation of exogenous peptide antigen via MHC class II GO:0019886 TAS
    regulation of interleukin-4 production GO:0032673 ISS
    positive regulation of insulin secretion involved in cellular response to glucose stimulus GO:0035774 ISS
    regulation of interleukin-10 secretion GO:2001179 ISS
    inflammatory response to antigenic stimulus GO:0002437 ISS
    T-helper 1 type immune response GO:0042088 ISS
    negative regulation of interferon-gamma production GO:0032689 ISS
    humoral immune response mediated by circulating immunoglobulin GO:0002455 ISS
    protein tetramerization GO:0051262 ISS
    immune response GO:0006955 ISS
    interferon-gamma-mediated signaling pathway GO:0060333 TAS
    detection of bacterium GO:0016045 ISS
    cytokine-mediated signaling pathway GO:0019221 TAS
Subcellular Localization
    extracellular space GO:0005615 IDA
    endocytic vesicle membrane GO:0030666 TAS
    plasma membrane GO:0005886 TAS
    clathrin-coated endocytic vesicle membrane GO:0030669 TAS
    integral component of lumenal side of endoplasmic reticulum membrane GO:0071556 TAS
    membrane GO:0016020 IDA
    lysosomal membrane GO:0005765 TAS
    transport vesicle membrane GO:0030658 TAS
    external side of plasma membrane GO:0009897 ISS
    extracellular exosome GO:0070062 IDA
    MHC class II protein complex GO:0042613 ISS
    late endosome membrane GO:0031902 IDA
    trans-Golgi network membrane GO:0032588 TAS
    Golgi membrane GO:0000139 TAS
    ER to Golgi transport vesicle membrane GO:0012507 TAS
 Experiment description of studies that identified HLA-DRB5 in exosomes
1
Experiment ID 11
ISEV standards
✘ EV Biophysical techniques
✔
HSC70|HSP90
EV Cytosolic markers
✔
MHCII|CD63|CD81
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [MALDI TOF]
Western blotting
PubMed ID 12519789    
Organism Homo sapiens
Experiment description Proteomic and biochemical analyses of human B cell-derived exosomes. Potential implications for their function and multivesicular body formation.
Authors Wubbolts R, Leckie RS, Veenhuizen PT, Schwarzmann G, Mobius W, Hoernschemeyer J, Slot JW, Geuze HJ, Stoorvogel W
Journal name JBC
Publication year 2003
Sample B cells
Sample name RN (HLA-DR15+)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [MALDI TOF]
Mass spectrometry [QTOF]
Western blotting
Thin layer chromatography
HPLC
2
Experiment ID 16
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
MHCII|CD86|LAMP1|LAMP2|CD63|CD81|CD82|ICAM1|TFRC
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Western blotting
Immunoelectron Microscopy
PubMed ID 9685355    
Organism Homo sapiens
Experiment description Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes.
Authors Escola JM, Kleijmeer MJ, Stoorvogel W, Griffith JM, Yoshie O, Geuze HJ
Journal name JBC
Publication year 1998
Sample B cells
Sample name RN (HLA-DR15+)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.12-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Immunoelectron Microscopy
3
Experiment ID 79
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD81|MHCII
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
4
Experiment ID 80
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD81|MHCII
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
5
Experiment ID 81
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD81|MHCII
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
6
Experiment ID 124
ISEV standards
✔
IEM|EM
EV Biophysical techniques
✔
HSC70
EV Cytosolic markers
✔
CD63|CD9|CD81
EV Membrane markers
✔
CANX
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method FACS
PubMed ID 21235781    
Organism Homo sapiens
Experiment description Human saliva, plasma and breast milk exosomes contain RNA: uptake by macrophages.
Authors Lasser C, Alikhani VS, Ekstrom K, Eldh M, Paredes PT, Bossios A, Sjostrand M, Gabrielsson S, Lotvall J, Valadi H.
Journal name JTM
Publication year 2011
Sample Breast milk
Sample name Breast milk - Normal
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study FACS
Immunoelectron Microscopy
Western blotting
7
Experiment ID 123
ISEV standards
✔
IEM|EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63|CD9|CD81
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method FACS
PubMed ID 21235781    
Organism Homo sapiens
Experiment description Human saliva, plasma and breast milk exosomes contain RNA: uptake by macrophages.
Authors Lasser C, Alikhani VS, Ekstrom K, Eldh M, Paredes PT, Bossios A, Sjostrand M, Gabrielsson S, Lotvall J, Valadi H.
Journal name JTM
Publication year 2011
Sample Plasma
Sample name Plasma - Normal
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study FACS
Immunoelectron Microscopy
Western blotting
8
Experiment ID 231
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 232
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 233
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 122
ISEV standards
✔
IEM|EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63|CD9|CD81
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method FACS
PubMed ID 21235781    
Organism Homo sapiens
Experiment description Human saliva, plasma and breast milk exosomes contain RNA: uptake by macrophages.
Authors Lasser C, Alikhani VS, Ekstrom K, Eldh M, Paredes PT, Bossios A, Sjostrand M, Gabrielsson S, Lotvall J, Valadi H.
Journal name JTM
Publication year 2011
Sample Saliva
Sample name Saliva - Normal
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study FACS
Immunoelectron Microscopy
Western blotting
12
Experiment ID 18
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method FACS
PubMed ID 11907077    
Organism Homo sapiens
Experiment description TCR activation of human T cells induces the production of exosomes bearing the TCR/CD3/zeta complex.
Authors Blanchard N, Lankar D, Faure F, Regnault A, Dumont C, Raposo G, Hivroz C
Journal name JIMMU
Publication year 2002
Sample T cells
Sample name Jurkat
T cell blasts
Isolation/purification methods Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study FACS
13
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for HLA-DRB5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HLA-DRA 3122
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here