Gene description for U2af2
Gene name U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 2
Gene symbol U2af2
Other names/aliases 65kDa
Species Mus musculus
 Database cross references - U2af2
ExoCarta ExoCarta_22185
Vesiclepedia VP_22185
Entrez Gene 22185
UniProt P26369  
 U2af2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for U2af2
Molecular Function
    protein binding GO:0005515 IPI
    poly-pyrimidine tract binding GO:0008187 IBA
    enzyme binding GO:0019899 IEA
    enzyme binding GO:0019899 ISO
    pre-mRNA 3'-splice site binding GO:0030628 IBA
    pre-mRNA 3'-splice site binding GO:0030628 IEA
    C2H2 zinc finger domain binding GO:0070742 IPI
    molecular function inhibitor activity GO:0140678 ISO
Biological Process
    spliceosomal complex assembly GO:0000245 IBA
    mRNA splicing, via spliceosome GO:0000398 NAS
    RNA splicing GO:0008380 IDA
    negative regulation of protein ubiquitination GO:0031397 IEA
    negative regulation of protein ubiquitination GO:0031397 ISO
    positive regulation of RNA splicing GO:0033120 ISO
    positive regulation of RNA splicing GO:0033120 ISS
    negative regulation of mRNA splicing, via spliceosome GO:0048025 ISO
    negative regulation of mRNA splicing, via spliceosome GO:0048025 ISS
Subcellular Localization
    commitment complex GO:0000243 IBA
    Prp19 complex GO:0000974 ISO
    Prp19 complex GO:0000974 ISS
    nucleus GO:0005634 NAS
    spliceosomal complex GO:0005681 ISO
    nuclear speck GO:0016607 IBA
    nuclear speck GO:0016607 ISO
    nuclear speck GO:0016607 TAS
    U2-type prespliceosome GO:0071004 IBA
    U2-type prespliceosome GO:0071004 IEA
    U2AF complex GO:0089701 IBA
    U2AF complex GO:0089701 ISO
 Experiment description of studies that identified U2af2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for U2af2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Sf3a3 75062
Co-fractionation Mus musculus
2 ZRSR2  
Two-hybrid Homo sapiens
3 2810403A07Rik  
Co-fractionation Mus musculus
4 Qk  
Co-fractionation Mus musculus
5 Ddx39 68278
Two-hybrid Mus musculus
6 Snrpd1 20641
Co-fractionation Mus musculus
7 SUGP1  
Two-hybrid Homo sapiens
8 Celf1  
Two-hybrid Mus musculus
9 Fubp3  
Two-hybrid Mus musculus
10 Rtcb 28088
Two-hybrid Mus musculus
11 Sf3b1 81898
Co-fractionation Mus musculus
12 Snrpd2 107686
Co-fractionation Mus musculus
13 Atxn1  
Affinity Capture-MS Mus musculus
14 Rbm39 170791
Co-fractionation Mus musculus
15 SNRPA 6626
Two-hybrid Homo sapiens
16 SF1 7536
Two-hybrid Homo sapiens
17 ALDOA 226
Two-hybrid Homo sapiens
18 Snrnp200 320632
Co-fractionation Mus musculus
19 SRPK2 6733
Two-hybrid Homo sapiens
20 Sf3a1 67465
Co-fractionation Mus musculus
21 PUF60 22827
Two-hybrid Homo sapiens
22 Snrpd3 67332
Co-fractionation Mus musculus
23 Sf3b3 101943
Co-fractionation Mus musculus
24 Sf1 22668
Co-fractionation Mus musculus
25 Mettl21e  
Affinity Capture-MS Mus musculus
26 Snrpe 20643
Co-fractionation Mus musculus
27 CPEB2  
Two-hybrid Homo sapiens
28 KHDRBS2  
Two-hybrid Homo sapiens
29 Srsf1 110809
Co-fractionation Mus musculus
30 PRPF19 27339
Two-hybrid Homo sapiens
31 Puf60 67959
Co-fractionation Mus musculus
32 Snrpa  
Co-fractionation Mus musculus
33 Snw1  
Co-fractionation Mus musculus
34 Phf5a  
Co-fractionation Mus musculus
35 Sfpq 71514
Co-fractionation Mus musculus
36 Dhx15 13204
Co-fractionation Mus musculus
37 Fancd2  
Affinity Capture-MS Mus musculus
38 SNRPD1 6632
Two-hybrid Homo sapiens
39 Hnrnpk 15387
Two-hybrid Mus musculus
40 Ddx42 72047
Two-hybrid Mus musculus
41 Eed  
Affinity Capture-MS Mus musculus
42 Hnrnpc 15381
Co-fractionation Mus musculus
43 Ptbp1 19205
Co-fractionation Mus musculus
44 Srsf3 20383
Two-hybrid Mus musculus
45 Mapt  
Affinity Capture-MS Mus musculus
46 Tia1  
Affinity Capture-MS Mus musculus
47 Tcerg1  
Co-fractionation Mus musculus
48 Otx2  
Affinity Capture-MS Mus musculus
49 Hnrnpa2b1 53379
Co-fractionation Mus musculus
50 Tcea1 21399
Co-fractionation Mus musculus
51 U2AF1 7307
Two-hybrid Homo sapiens
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