Gene description for U2af2
Gene name U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 2
Gene symbol U2af2
Other names/aliases 65kDa
Species Mus musculus
 Database cross references - U2af2
ExoCarta ExoCarta_22185
Vesiclepedia VP_22185
Entrez Gene 22185
UniProt P26369  
 U2af2 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for U2af2
Molecular Function
    poly(A) RNA binding GO:0044822 ISO
    enzyme binding GO:0019899 ISO
    nucleic acid binding GO:0003676 IEA
    protein binding GO:0005515 IPI
    nucleotide binding GO:0000166 IEA
    C2H2 zinc finger domain binding GO:0070742 IPI
    RNA binding GO:0003723 IEA
Biological Process
    regulation of mitochondrion degradation GO:1903146 ISO
    mRNA processing GO:0006397 IEA
    positive regulation of RNA splicing GO:0033120 ISO
    negative regulation of mRNA splicing, via spliceosome GO:0048025 ISO
    positive regulation of protein targeting to mitochondrion GO:1903955 ISO
    RNA splicing GO:0008380 IDA
Subcellular Localization
    nucleus GO:0005634 IEA
    nucleoplasm GO:0005654 ISO
    Prp19 complex GO:0000974 ISO
    spliceosomal complex GO:0005681 ISO
    nuclear speck GO:0016607 TAS
 Experiment description of studies that identified U2af2 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for U2af2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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