Gene description for Ddx39
Gene name DEAD (Asp-Glu-Ala-Asp) box polypeptide 39
Gene symbol Ddx39
Other names/aliases 2610307C23Rik
BAT1
DDXL
Ddx39a
URH49
Species Mus musculus
 Database cross references - Ddx39
ExoCarta ExoCarta_68278
Vesiclepedia VP_68278
Entrez Gene 68278
UniProt Q8VDW0  
 Ddx39 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ddx39
Molecular Function
    nucleic acid binding GO:0003676 IEA
    DNA clamp loader activity GO:0003689 IEA
    RNA helicase activity GO:0003724 IBA
    RNA helicase activity GO:0003724 IEA
    mRNA binding GO:0003729 IBA
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IEA
    ATP hydrolysis activity GO:0016887 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    cohesin loader activity GO:0061775 IEA
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
Biological Process
    mRNA splicing, via spliceosome GO:0000398 IBA
    mRNA splicing, via spliceosome GO:0000398 IEA
    mRNA splicing, via spliceosome GO:0000398 ISO
    chromatin remodeling GO:0006338 IEA
    mRNA export from nucleus GO:0006406 IBA
    mRNA export from nucleus GO:0006406 IEA
    mRNA export from nucleus GO:0006406 ISO
    chromatin looping GO:0140588 IEA
Subcellular Localization
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 IEA
    cytoplasm GO:0005737 ISO
    nuclear speck GO:0016607 IEA
    nuclear speck GO:0016607 ISO
 Experiment description of studies that identified Ddx39 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ddx39
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ube2i  
Two-hybrid Mus musculus
2 SNRPA 6626
Two-hybrid Homo sapiens
3 Uba5  
Co-fractionation Mus musculus
4 PRPF6 24148
Two-hybrid Homo sapiens
5 RING1 6015
Two-hybrid Homo sapiens
6 Eed  
Affinity Capture-MS Mus musculus
7 PTBP2  
Two-hybrid Homo sapiens
8 Yars 107271
Two-hybrid Mus musculus
9 U2af2 22185
Two-hybrid Mus musculus
10 IPO5 3843
Two-hybrid Homo sapiens
11 Atg16l1  
Affinity Capture-MS Mus musculus
12 Eif1a  
Two-hybrid Mus musculus
13 RPL23 9349
Two-hybrid Homo sapiens
14 Fancd2  
Affinity Capture-MS Mus musculus
15 SARNP 84324
Two-hybrid Homo sapiens
View the network image/svg+xml



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