Gene description for Ddx42
Gene name DEAD (Asp-Glu-Ala-Asp) box polypeptide 42
Gene symbol Ddx42
Other names/aliases 1810047H21Rik
AW319508
AW556242
B430002H05Rik
RHELP
RNAHP
SF3b125
Species Mus musculus
 Database cross references - Ddx42
ExoCarta ExoCarta_72047
Vesiclepedia VP_72047
Entrez Gene 72047
UniProt Q810A7  
 Ddx42 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Ddx42
Molecular Function
    DNA clamp loader activity GO:0003689 IEA
    RNA binding GO:0003723 IEA
    RNA binding GO:0003723 ISO
    RNA helicase activity GO:0003724 IEA
    RNA helicase activity GO:0003724 ISO
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IEA
    cohesin loader activity GO:0061775 IEA
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    protein localization GO:0008104 ISO
    protein localization GO:0008104 ISS
    regulation of apoptotic process GO:0042981 ISO
    regulation of apoptotic process GO:0042981 ISS
    chromatin looping GO:0140588 IEA
    U2-type prespliceosome assembly GO:1903241 ISO
    U2-type prespliceosome assembly GO:1903241 ISS
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    nuclear speck GO:0016607 IEA
    nuclear speck GO:0016607 ISO
    U2-type prespliceosome GO:0071004 ISO
    U2-type prespliceosome GO:0071004 ISS
 Experiment description of studies that identified Ddx42 in exosomes
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Ddx42
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Dhx15 13204
Co-fractionation Mus musculus
2 Sf3b1 81898
Co-fractionation Mus musculus
3 Tmem173  
Proximity Label-MS Mus musculus
4 Atxn1  
Proximity Label-MS Mus musculus
5 RBFOX2 23543
Two-hybrid Homo sapiens
6 Eed  
Affinity Capture-MS Mus musculus
7 Sf3b3 101943
Co-fractionation Mus musculus
8 U2af2 22185
Two-hybrid Mus musculus
9 Atg16l1  
Affinity Capture-MS Mus musculus
10 Prpf19 28000
Co-fractionation Mus musculus
11 Sf3a1 67465
Co-fractionation Mus musculus
12 Cdc5l 71702
Co-fractionation Mus musculus
13 RBM17 84991
Two-hybrid Homo sapiens
14 PUF60 22827
Two-hybrid Homo sapiens
View the network image/svg+xml



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