Gene description for UBE4A
Gene name ubiquitination factor E4A
Gene symbol UBE4A
Other names/aliases E4
UBOX2
UFD2
Species Homo sapiens
 Database cross references - UBE4A
ExoCarta ExoCarta_9354
Vesiclepedia VP_9354
Entrez Gene 9354
HGNC 12499
MIM 603753
UniProt Q14139  
 UBE4A identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for UBE4A
Molecular Function
    protein binding GO:0005515 IPI
    ubiquitin-ubiquitin ligase activity GO:0034450 IBA
Biological Process
    protein polyubiquitination GO:0000209 IBA
    ubiquitin-dependent protein catabolic process GO:0006511 IEA
    ERAD pathway GO:0036503 IBA
Subcellular Localization
    ubiquitin ligase complex GO:0000151 IEA
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 IBA
 Experiment description of studies that identified UBE4A in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for UBE4A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SV2C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 GYPA  
Affinity Capture-MS Homo sapiens
3 TTYH1  
Affinity Capture-MS Homo sapiens
4 MAVS 57506
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
5 KCNMB3  
Affinity Capture-MS Homo sapiens
6 FASN 2194
Positive Genetic Homo sapiens
7 GYPB  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 VAPB 9217
Affinity Capture-MS Homo sapiens
9 GPR17 2840
Affinity Capture-MS Homo sapiens
10 SIGLECL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 SLA  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
12 KIR2DL1  
Affinity Capture-MS Homo sapiens
13 C3orf52  
Affinity Capture-MS Homo sapiens
14 ZDHHC12  
Affinity Capture-MS Homo sapiens
15 LRRC25  
Affinity Capture-MS Homo sapiens
16 FOXB1  
Affinity Capture-MS Homo sapiens
17 TMEM134  
Affinity Capture-MS Homo sapiens
18 VSIG1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 CD70 970
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 IL9R  
Affinity Capture-MS Homo sapiens
21 CHRNA1  
Affinity Capture-MS Homo sapiens
22 FAM3C 10447
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
23 EDEM1  
Affinity Capture-MS Homo sapiens
24 PRDM1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 SPSB4  
Affinity Capture-MS Homo sapiens
26 C9orf78 51759
Affinity Capture-MS Homo sapiens
27 EFNB1 1947
Affinity Capture-MS Homo sapiens
28 C19orf38  
Affinity Capture-MS Homo sapiens
29 TP53 7157
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
30 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
31 FOXI2  
Affinity Capture-MS Homo sapiens
32 UBE2D4  
Two-hybrid Homo sapiens
33 FOXL1  
Affinity Capture-MS Homo sapiens
34 C1orf210  
Affinity Capture-MS Homo sapiens
35 ITFG3 83986
Affinity Capture-MS Homo sapiens
36 LOC100132735  
Protein-RNA Homo sapiens
37 GZMB  
Biochemical Activity Homo sapiens
38 PRKCSH 5589
Co-fractionation Homo sapiens
39 UBE2D2 7322
Two-hybrid Homo sapiens
40 FGFR2 2263
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 PTP4A3  
Affinity Capture-MS Homo sapiens
42 PTTG1  
Biochemical Activity Homo sapiens
43 B4GALT3 8703
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 SEC61B 10952
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 FOXL2  
Affinity Capture-MS Homo sapiens
46 PTPN1 5770
Proximity Label-MS Homo sapiens
47 MDM2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
48 NTRK1 4914
Affinity Capture-MS Homo sapiens
49 CASP6 839
Biochemical Activity Homo sapiens
50 LRRTM1 347730
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 PRAMEF9  
Affinity Capture-MS Homo sapiens
52 EPHA2 1969
Biochemical Activity Homo sapiens
53 IP6K1 9807
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
54 DNAJC1 64215
Proximity Label-MS Homo sapiens
55 C6orf62 81688
Affinity Capture-MS Homo sapiens
56 KCNA2  
Affinity Capture-MS Homo sapiens
57 FOXQ1  
Affinity Capture-MS Homo sapiens
58 ATP1B3 483
Affinity Capture-MS Homo sapiens
59 C3orf18  
Affinity Capture-MS Homo sapiens
60 CUL5 8065
Affinity Capture-MS Homo sapiens
61 VIMP 55829
Affinity Capture-MS Homo sapiens
62 SEMA4C 54910
Affinity Capture-MS Homo sapiens
63 WDHD1  
Co-fractionation Homo sapiens
64 PARP16 54956
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 UBXN4 23190
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 UBE2D3 7323
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
67 UBE2D1 7321
Two-hybrid Homo sapiens
68 VCP 7415
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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