Gene description for B3GALNT2
Gene name beta-1,3-N-acetylgalactosaminyltransferase 2
Gene symbol B3GALNT2
Other names/aliases B3GalNAc-T2
MDDGA11
Species Homo sapiens
 Database cross references - B3GALNT2
ExoCarta ExoCarta_148789
Vesiclepedia VP_148789
Entrez Gene 148789
HGNC 28596
MIM 610194
UniProt Q8NCR0  
 B3GALNT2 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for B3GALNT2
Molecular Function
    protein binding GO:0005515 IPI
    UDP-glycosyltransferase activity GO:0008194 IBA
    acetylgalactosaminyltransferase activity GO:0008376 IDA
    acetylgalactosaminyltransferase activity GO:0008376 TAS
Biological Process
    protein glycosylation GO:0006486 IMP
    protein O-linked glycosylation GO:0006493 IBA
    protein O-linked glycosylation GO:0006493 IDA
    protein O-linked glycosylation GO:0006493 TAS
Subcellular Localization
    Golgi membrane GO:0000139 IBA
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum membrane GO:0005789 TAS
 Experiment description of studies that identified B3GALNT2 in exosomes
1
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for B3GALNT2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NRG1 3084
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 CFAP69  
Affinity Capture-MS Homo sapiens
3 SFRP4 6424
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 YY1AP1 55249
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 IFNL3  
Affinity Capture-MS Homo sapiens
6 CD3D 915
Affinity Capture-MS Homo sapiens
7 NUBP2 10101
Affinity Capture-MS Homo sapiens
8 TAZ  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 CHST8 64377
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 EIF2B2 8892
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 TMBIM1 64114
Two-hybrid Homo sapiens
12 AGMAT  
Affinity Capture-MS Homo sapiens
13 CUL3 8452
Affinity Capture-MS Homo sapiens
14 FBXO6 26270
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 OS9 10956
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 NXF1 10482
Affinity Capture-RNA Homo sapiens
17 AMIGO1  
Affinity Capture-MS Homo sapiens
18 PLD3 23646
Affinity Capture-MS Homo sapiens
19 IDS  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 FBXO2 26232
Affinity Capture-MS Homo sapiens
21 FBXW7  
Synthetic Lethality Homo sapiens
22 KLF12  
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 PARP16 54956
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 GFRA3 2676
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which B3GALNT2 is involved
PathwayEvidenceSource
Metabolism of proteins TAS Reactome
O-linked glycosylation TAS Reactome
Post-translational protein modification TAS Reactome





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