Gene description for GPC3
Gene name glypican 3
Gene symbol GPC3
Other names/aliases DGSX
GTR2-2
MXR7
OCI-5
SDYS
SGB
SGBS
SGBS1
Species Homo sapiens
 Database cross references - GPC3
ExoCarta ExoCarta_2719
Vesiclepedia VP_2719
Entrez Gene 2719
HGNC 4451
MIM 300037
UniProt P51654  
 GPC3 identified in exosomes derived from the following tissue/cell type
Liver cancer cells 25265333    
 Gene ontology annotations for GPC3
Molecular Function
    protein binding GO:0005515 IPI
    peptidyl-dipeptidase inhibitor activity GO:0060422 IDA
Biological Process
    branching involved in ureteric bud morphogenesis GO:0001658 IEA
    smoothened signaling pathway GO:0007224 IEA
    response to bacterium GO:0009617 IEA
    anatomical structure morphogenesis GO:0009653 TAS
    anterior/posterior axis specification GO:0009948 IEA
    body morphogenesis GO:0010171 IEA
    cell migration GO:0016477 IBA
    bone mineralization GO:0030282 IEA
    osteoclast differentiation GO:0030316 IEA
    lung development GO:0030324 IEA
    positive regulation of BMP signaling pathway GO:0030513 IEA
    embryonic hindlimb morphogenesis GO:0035116 IEA
    cell migration involved in gastrulation GO:0042074 ISS
    positive regulation of protein catabolic process GO:0045732 ISS
    positive regulation of endocytosis GO:0045807 ISS
    negative regulation of smoothened signaling pathway GO:0045879 ISS
    positive regulation of smoothened signaling pathway GO:0045880 IEA
    negative regulation of growth GO:0045926 IEA
    positive regulation of D-glucose import GO:0046326 IEA
    epithelial cell proliferation GO:0050673 IEA
    negative regulation of epithelial cell proliferation GO:0050680 IEA
    canonical Wnt signaling pathway GO:0060070 IEA
    Wnt signaling pathway, planar cell polarity pathway GO:0060071 IEA
    regulation of canonical Wnt signaling pathway GO:0060828 IDA
    coronary vasculature development GO:0060976 ISS
    cell proliferation involved in kidney development GO:0072111 ISS
    mesenchymal cell proliferation involved in ureteric bud development GO:0072138 ISS
    mesonephric duct morphogenesis GO:0072180 ISS
    cell proliferation involved in metanephros development GO:0072203 IEA
    negative regulation of canonical Wnt signaling pathway GO:0090090 IEA
    positive regulation of canonical Wnt signaling pathway GO:0090263 IBA
    positive regulation of canonical Wnt signaling pathway GO:0090263 IDA
    regulation of protein localization to membrane GO:1905475 IBA
    regulation of non-canonical Wnt signaling pathway GO:2000050 IDA
    positive regulation of Wnt signaling pathway, planar cell polarity pathway GO:2000096 IEA
Subcellular Localization
    endoplasmic reticulum lumen GO:0005788 TAS
    Golgi lumen GO:0005796 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISS
    plasma membrane GO:0005886 TAS
    cell surface GO:0009986 IBA
    lysosomal lumen GO:0043202 TAS
    collagen-containing extracellular matrix GO:0062023 IEA
    side of membrane GO:0098552 IEA
 Experiment description of studies that identified GPC3 in exosomes
1
Experiment ID 267
MISEV standards
Biophysical techniques
TSG101|Alix
Enriched markers
CANX|HSP90B1
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25265333    
Organism Homo sapiens
Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh-7 Cells Using SILAC Labeling and LC-MS/MS
Authors "Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J"
Journal name J Proteome Res
Publication year 2014
Sample Liver cancer cells
Sample name Huh-7 - Transfected with HBx plasmids
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for GPC3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LRRTM2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 CAMKV 79012
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 C7orf34  
Affinity Capture-MS Homo sapiens
4 LMAN2 10960
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 GPIHBP1  
Affinity Capture-MS Homo sapiens
6 ZFPL1 7542
Affinity Capture-MS Homo sapiens
7 PRDM10  
Affinity Capture-MS Homo sapiens
8 PIP5K1A 8394
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 ST14 6768
Affinity Capture-MS Homo sapiens
10 GNPAT 8443
Affinity Capture-MS Homo sapiens
11 HADHA 3030
Affinity Capture-MS Homo sapiens
12 CANX 821
Affinity Capture-MS Homo sapiens
13 SIGLECL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 FOLR1 2348
Affinity Capture-MS Homo sapiens
15 ULBP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 FGF2 2247
Affinity Capture-Western Homo sapiens
17 KCTD10 83892
Affinity Capture-MS Homo sapiens
18 RAB34 83871
Affinity Capture-MS Homo sapiens
19 TAZ  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 HPN  
Affinity Capture-MS Homo sapiens
21 HMOX2 3163
Affinity Capture-MS Homo sapiens
22 VKORC1L1 154807
Affinity Capture-MS Homo sapiens
23 MOXD1 26002
Affinity Capture-MS Homo sapiens
24 EMC4 51234
Affinity Capture-MS Homo sapiens
25 CLGN 1047
Affinity Capture-MS Homo sapiens
26 COL20A1  
Affinity Capture-MS Homo sapiens
27 RLN1  
Affinity Capture-MS Homo sapiens
28 TMEM186  
Affinity Capture-MS Homo sapiens
29 C4orf26  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 NT5C3A 51251
Affinity Capture-MS Homo sapiens
31 PCYOX1 51449
Affinity Capture-MS Homo sapiens
32 TSPYL1 7259
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 TUB  
Affinity Capture-MS Homo sapiens
34 RIPK4  
Affinity Capture-MS Homo sapiens
35 TRIM68 55128
Affinity Capture-MS Homo sapiens
36 CLEC12B  
Affinity Capture-MS Homo sapiens
37 GPR183  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 RAET1L  
Affinity Capture-MS Homo sapiens
39 LRRTM4  
Affinity Capture-MS Homo sapiens
40 PARP16 54956
Affinity Capture-MS Homo sapiens
41 PTPRS 5802
Reconstituted Complex Homo sapiens
42 SCGB2A2  
Affinity Capture-MS Homo sapiens
43 PSCA 8000
Affinity Capture-MS Homo sapiens
44 REEP6  
Affinity Capture-MS Homo sapiens
45 RAP1A 5906
Affinity Capture-MS Homo sapiens
46 NR2F2  
Affinity Capture-MS Homo sapiens
47 FGF4  
Affinity Capture-MS Homo sapiens
48 DNAJC1 64215
Affinity Capture-MS Homo sapiens
49 ZNF460  
Affinity Capture-MS Homo sapiens
50 DNASE1L1 1774
Affinity Capture-MS Homo sapiens
51 NRN1  
Affinity Capture-MS Homo sapiens
52 ISLR 3671
Affinity Capture-MS Homo sapiens
53 NOG 9241
Affinity Capture-MS Homo sapiens
54 XYLT2 64132
Affinity Capture-MS Homo sapiens
55 ENPP1 5167
Affinity Capture-MS Homo sapiens
56 FGF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 LRRTM3  
Affinity Capture-MS Homo sapiens
58 DAXX  
Affinity Capture-MS Homo sapiens
59 TULP3 7289
Affinity Capture-MS Homo sapiens
60 MUL1  
Affinity Capture-MS Homo sapiens
61 LIPG 9388
Affinity Capture-MS Homo sapiens
62 REEP5 7905
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which GPC3 is involved
PathwayEvidenceSource
A tetrasaccharide linker sequence is required for GAG synthesis TAS Reactome
Attachment and Entry TAS Reactome
Attachment and Entry IEA Reactome
Chondroitin sulfate/dermatan sulfate metabolism TAS Reactome
Defective B3GALT6 causes EDSP2 and SEMDJL1 TAS Reactome
Defective B3GAT3 causes JDSSDHD TAS Reactome
Defective B4GALT7 causes EDS, progeroid type TAS Reactome
Defective EXT1 causes exostoses 1, TRPS2 and CHDS TAS Reactome
Defective EXT2 causes exostoses 2 TAS Reactome
Disease TAS Reactome
Disease IEA Reactome
Diseases associated with glycosaminoglycan metabolism TAS Reactome
Diseases of glycosylation TAS Reactome
Diseases of metabolism TAS Reactome
Early SARS-CoV-2 Infection Events TAS Reactome
Early SARS-CoV-2 Infection Events IEA Reactome
Glycosaminoglycan metabolism TAS Reactome
Glycosaminoglycan metabolism IEA Reactome
Heparan sulfate/heparin (HS-GAG) metabolism TAS Reactome
Heparan sulfate/heparin (HS-GAG) metabolism IEA Reactome
HS-GAG biosynthesis TAS Reactome
HS-GAG biosynthesis IEA Reactome
HS-GAG degradation TAS Reactome
Infectious disease TAS Reactome
Infectious disease IEA Reactome
Metabolism TAS Reactome
Metabolism IEA Reactome
Metabolism of carbohydrates TAS Reactome
Metabolism of carbohydrates IEA Reactome
Metabolism of fat-soluble vitamins TAS Reactome
Metabolism of proteins TAS Reactome
Metabolism of vitamins and cofactors TAS Reactome
Post-translational protein modification TAS Reactome
Post-translational protein phosphorylation TAS Reactome
Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) TAS Reactome
Respiratory syncytial virus (RSV) attachment and entry TAS Reactome
Respiratory Syncytial Virus Infection Pathway TAS Reactome
Retinoid metabolism and transport TAS Reactome
RSV-host interactions TAS Reactome
SARS-CoV Infections TAS Reactome
SARS-CoV Infections IEA Reactome
SARS-CoV-2 Infection TAS Reactome
SARS-CoV-2 Infection IEA Reactome
Sensory Perception TAS Reactome
Viral Infection Pathways TAS Reactome
Viral Infection Pathways IEA Reactome
Visual phototransduction TAS Reactome





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