Gene description for CLDN2
Gene name claudin 2
Gene symbol CLDN2
Other names/aliases -
Species Homo sapiens
 Database cross references - CLDN2
ExoCarta ExoCarta_9075
Vesiclepedia VP_9075
Entrez Gene 9075
HGNC 2041
MIM 300520
UniProt P57739  
 CLDN2 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 34887515    
 Gene ontology annotations for CLDN2
Molecular Function
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 ISS
    structural molecule activity GO:0005198 IEA
Biological Process
    calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules GO:0016338 ISS
Subcellular Localization
    extracellular exosome GO:0070062 IDA
    bicellular tight junction GO:0005923 ISS
    plasma membrane GO:0005886 IEA
    integral component of membrane GO:0016021 IEA
 Experiment description of studies that identified CLDN2 in exosomes
1
Experiment ID 282
MISEV standards
✔
CEM
EV Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
EV Enriched markers
✘ EV Negative markers
✔
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 283
MISEV standards
✔
CEM
EV Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
EV Enriched markers
✘ EV Negative markers
✔
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
3
Experiment ID 1203
MISEV standards
✔
EM
EV Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ.
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
 Protein-protein interactions for CLDN2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KRTAP4-12  
Two-hybrid Homo sapiens
2 TJP1 7082
Invitro Homo sapiens
View the network image/svg+xml
 Pathways in which CLDN2 is involved
PathwayEvidenceSource
Tight junction interactions TAS Reactome





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