Gene description for SIGLEC9
Gene name sialic acid binding Ig-like lectin 9
Gene symbol SIGLEC9
Other names/aliases CD329
CDw329
FOAP-9
OBBP-LIKE
siglec-9
Species Homo sapiens
 Database cross references - SIGLEC9
ExoCarta ExoCarta_27180
Vesiclepedia VP_27180
Entrez Gene 27180
HGNC 10878
MIM 605640
UniProt Q9Y336  
 SIGLEC9 identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for SIGLEC9
Molecular Function
    protein binding GO:0005515 IPI
    carbohydrate binding GO:0030246 NAS
    sialic acid binding GO:0033691 IBA
Biological Process
    cell adhesion GO:0007155 IBA
    cell surface receptor signaling pathway GO:0007166 NAS
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 NAS
    plasma membrane GO:0005886 TAS
    secretory granule membrane GO:0030667 TAS
 Experiment description of studies that identified SIGLEC9 in exosomes
1
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for SIGLEC9
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BRICD5  
Two-hybrid Homo sapiens
2 NCS1 23413
Two-hybrid Homo sapiens
3 SFTPC  
Two-hybrid Homo sapiens
4 NCAM1 4684
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 LGALS3BP 3959
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml



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