Gene description for PI4KB
Gene name phosphatidylinositol 4-kinase, catalytic, beta
Gene symbol PI4KB
Other names/aliases NPIK
PI4K-BETA
PI4K92
PI4KBETA
PI4KIIIBETA
PIK4CB
Species Homo sapiens
 Database cross references - PI4KB
ExoCarta ExoCarta_5298
Vesiclepedia VP_5298
Entrez Gene 5298
HGNC 8984
MIM 602758
UniProt Q9UBF8  
 PI4KB identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for PI4KB
Molecular Function
    1-phosphatidylinositol 4-kinase activity GO:0004430 IBA
    1-phosphatidylinositol 4-kinase activity GO:0004430 IDA
    1-phosphatidylinositol 4-kinase activity GO:0004430 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    14-3-3 protein binding GO:0071889 IDA
Biological Process
    phosphatidylinositol biosynthetic process GO:0006661 TAS
    receptor-mediated endocytosis GO:0006898 TAS
    lysosome organization GO:0007040 IDA
    signal transduction GO:0007165 TAS
    phosphatidylinositol phosphate biosynthetic process GO:0046854 IBA
    phosphatidylinositol-mediated signaling GO:0048015 IBA
    inner ear development GO:0048839 IBA
    inner ear development GO:0048839 IMP
Subcellular Localization
    Golgi membrane GO:0000139 IDA
    Golgi membrane GO:0000139 TAS
    cytoplasm GO:0005737 IBA
    mitochondrial outer membrane GO:0005741 IEA
    endosome GO:0005768 TAS
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 TAS
    membrane GO:0016020 IBA
    rough endoplasmic reticulum membrane GO:0030867 IEA
    perinuclear region of cytoplasm GO:0048471 IEA
 Experiment description of studies that identified PI4KB in exosomes
1
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 196
MISEV standards
EM
Biophysical techniques
Alix|TSG101|HSP70|CD9
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 197
MISEV standards
EM
Biophysical techniques
Alix|TSG101|HSP70|CD9
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PI4KB
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 IARS 3376
Cross-Linking-MS (XL-MS) Homo sapiens
3 SFN 2810
Affinity Capture-MS Homo sapiens
4 DNAH6 1768
Cross-Linking-MS (XL-MS) Homo sapiens
5 UTRN 7402
Affinity Capture-MS Homo sapiens
6 EBAG9 9166
Proximity Label-MS Homo sapiens
7 BKRF1  
Affinity Capture-MS
8 CDK7 1022
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 LSM1 27257
Affinity Capture-MS Homo sapiens
10 C10orf76  
Affinity Capture-Western Homo sapiens
11 LAMTOR1 55004
Proximity Label-MS Homo sapiens
12 RAB9A 9367
Proximity Label-MS Homo sapiens
13 XPO1 7514
Affinity Capture-MS Homo sapiens
14 YWHAB 7529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 S100A6 6277
Affinity Capture-MS Homo sapiens
16 RAB5A 5868
Proximity Label-MS Homo sapiens
17 NR3C1 2908
Proximity Label-MS Homo sapiens
18 CBY1  
Affinity Capture-MS Homo sapiens
19 KBTBD7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 APEX1 328
Affinity Capture-RNA Homo sapiens
21 RDM1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 CASP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 YWHAZ 7534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 MYC  
Dosage Lethality Homo sapiens
25 YWHAE 7531
Affinity Capture-MS Homo sapiens
26 MOCS1  
Affinity Capture-MS Homo sapiens
27 RAB11A 8766
Proximity Label-MS Homo sapiens
28 RAB3IP  
Affinity Capture-MS Homo sapiens
29 HSPA1A 3303
Affinity Capture-MS Homo sapiens
30 DNAJC10 54431
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 NCS1 23413
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
32 YWHAQ 10971
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 RAB11B 9230
Affinity Capture-MS Homo sapiens
35 GSPT1 2935
Co-fractionation Homo sapiens
36 RAB7A 7879
Proximity Label-MS Homo sapiens
37 STX6 10228
Proximity Label-MS Homo sapiens
38 PTAR1 375743
Synthetic Lethality Homo sapiens
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