Gene description for FZD7
Gene name frizzled class receptor 7
Gene symbol FZD7
Other names/aliases FzE3
Species Homo sapiens
 Database cross references - FZD7
ExoCarta ExoCarta_8324
Vesiclepedia VP_8324
Entrez Gene 8324
HGNC 4045
MIM 603410
UniProt O75084  
 FZD7 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for FZD7
Molecular Function
    G protein-coupled receptor activity GO:0004930 IEA
    frizzled binding GO:0005109 IPI
    protein binding GO:0005515 IPI
    phosphatidylinositol-4,5-bisphosphate binding GO:0005546 IDA
    Wnt-protein binding GO:0017147 IBA
    Wnt-protein binding GO:0017147 IPI
    Wnt-protein binding GO:0017147 NAS
    PDZ domain binding GO:0030165 IPI
    Wnt receptor activity GO:0042813 IBA
    Wnt receptor activity GO:0042813 IDA
    Wnt receptor activity GO:0042813 NAS
Biological Process
    regulation of DNA-templated transcription GO:0006355 IMP
    G protein-coupled receptor signaling pathway GO:0007186 IEA
    negative regulation of cell-substrate adhesion GO:0010812 IMP
    skeletal muscle satellite cell maintenance involved in skeletal muscle regeneration GO:0014834 IEA
    stem cell population maintenance GO:0019827 IMP
    neuron differentiation GO:0030182 ISS
    T cell differentiation in thymus GO:0033077 IEA
    substrate adhesion-dependent cell spreading GO:0034446 IEA
    non-canonical Wnt signaling pathway GO:0035567 IBA
    non-canonical Wnt signaling pathway GO:0035567 IMP
    positive regulation of phosphorylation GO:0042327 IDA
    negative regulation of ectodermal cell fate specification GO:0042666 IMP
    positive regulation of MAPK cascade GO:0043410 IMP
    positive regulation of DNA-templated transcription GO:0045893 IDA
    positive regulation of DNA-templated transcription GO:0045893 IMP
    positive regulation of JNK cascade GO:0046330 IC
    somatic stem cell division GO:0048103 IEA
    positive regulation of epithelial cell proliferation involved in wound healing GO:0060054 IMP
    canonical Wnt signaling pathway GO:0060070 IBA
    canonical Wnt signaling pathway GO:0060070 IDA
    canonical Wnt signaling pathway GO:0060070 IMP
    Wnt signaling pathway, planar cell polarity pathway GO:0060071 NAS
    mesenchymal to epithelial transition GO:0060231 IMP
    regulation of canonical Wnt signaling pathway GO:0060828 IMP
    cellular response to retinoic acid GO:0071300 ISS
    negative regulation of cardiac muscle cell differentiation GO:2000726 IGI
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 TAS
    intracellular membrane-bounded organelle GO:0043231 IDA
    recycling endosome membrane GO:0055038 IDA
 Experiment description of studies that identified FZD7 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 255
MISEV standards
EM
Biophysical techniques
TSG101|FLOT1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 256
MISEV standards
EM
Biophysical techniques
TSG101|FLOT1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 257
MISEV standards
EM
Biophysical techniques
TSG101|FLOT1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
14
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for FZD7
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NFXL1 152518
Affinity Capture-MS Homo sapiens
2 ADCY1 107
Two-hybrid Homo sapiens
3 VNN2  
Affinity Capture-MS Homo sapiens
4 UBQLN4 56893
Two-hybrid Homo sapiens
5 SLC35B1 10237
Two-hybrid Homo sapiens
6 TTYH1  
Affinity Capture-MS Homo sapiens
7 FZD2 2535
Affinity Capture-MS Homo sapiens
8 FFAR2  
Two-hybrid Homo sapiens
9 PRNP 5621
Two-hybrid Homo sapiens
10 PDAP1 11333
Two-hybrid Homo sapiens
11 SLC39A4 55630
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 MSMO1 6307
Affinity Capture-MS Homo sapiens
13 ADGRE5 976
Affinity Capture-MS Homo sapiens
14 NCALD 83988
Two-hybrid Homo sapiens
15 HPCAL1 3241
Two-hybrid Homo sapiens
16 UBQLN2 29978
Two-hybrid Homo sapiens
17 LAMP1 3916
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 CLPTM1 1209
Two-hybrid Homo sapiens
19 AIG1  
Two-hybrid Homo sapiens
20 TMEM9B 56674
Affinity Capture-MS Homo sapiens
21 DLG4  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
22 SCGB1C1  
Affinity Capture-MS Homo sapiens
23 CCDC88C 440193
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
24 ERGIC1 57222
Affinity Capture-MS Homo sapiens
25 SLC30A4 7782
Two-hybrid Homo sapiens
26 DVL1 1855
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
27 ATP2A3 489
Affinity Capture-MS Homo sapiens
28 FAM192A  
Two-hybrid Homo sapiens
29 LEUTX  
Two-hybrid Homo sapiens
30 ADGRG6 57211
Affinity Capture-MS Homo sapiens
31 SGTB  
Two-hybrid Homo sapiens
32 GNAS 2778
Two-hybrid Homo sapiens
33 TRIM68 55128
Affinity Capture-MS Homo sapiens
34 TMEM237 65062
Two-hybrid Homo sapiens
35 OR13J1  
Affinity Capture-MS Homo sapiens
36 GOLGA5 9950
Affinity Capture-MS Homo sapiens
37 ACP2 53
Affinity Capture-MS Homo sapiens
38 EI24  
Affinity Capture-MS Homo sapiens
39 ATP2B2 491
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 EPN1 29924
Affinity Capture-Western Homo sapiens
41 GNAI3 2773
Affinity Capture-Western Homo sapiens
42 TMEM9 252839
Affinity Capture-MS Homo sapiens
43 NCS1 23413
Two-hybrid Homo sapiens
44 TM9SF4 9777
Affinity Capture-MS Homo sapiens
45 SGTA 6449
Two-hybrid Homo sapiens
46 CREB1  
Affinity Capture-MS Homo sapiens
47 SIGMAR1 10280
Affinity Capture-MS Homo sapiens
48 CLN8 2055
Two-hybrid Homo sapiens
49 ATP2B4 493
Affinity Capture-MS Homo sapiens
50 UPK1A 11045
Affinity Capture-MS Homo sapiens
51 FGFR4 2264
Affinity Capture-MS Homo sapiens
52 UBQLN1 29979
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
53 OR1D4  
Affinity Capture-MS Homo sapiens
54 BRI3BP 140707
Affinity Capture-MS Homo sapiens
55 SEZ6L2 26470
Affinity Capture-MS Homo sapiens
56 TM9SF1 10548
Affinity Capture-MS Homo sapiens
57 CERS2 29956
Affinity Capture-MS Homo sapiens
58 ZACN  
Affinity Capture-MS Homo sapiens
59 AQP9  
Two-hybrid Homo sapiens
60 ATP2B1 490
Affinity Capture-MS Homo sapiens
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