Gene description for GOSR2
Gene name golgi SNAP receptor complex member 2
Gene symbol GOSR2
Other names/aliases Bos1
EPM6
GS27
Species Homo sapiens
 Database cross references - GOSR2
ExoCarta ExoCarta_9570
Vesiclepedia VP_9570
Entrez Gene 9570
HGNC 4431
MIM 604027
UniProt O14653  
 GOSR2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for GOSR2
Molecular Function
    SNARE binding GO:0000149 IBA
    SNAP receptor activity GO:0005484 IBA
    protein binding GO:0005515 IPI
Biological Process
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 IDA
    intra-Golgi vesicle-mediated transport GO:0006891 IBA
    intra-Golgi vesicle-mediated transport GO:0006891 IDA
    vesicle fusion GO:0006906 IBA
    protein transport GO:0015031 IEA
Subcellular Localization
    Golgi membrane GO:0000139 IDA
    Golgi membrane GO:0000139 TAS
    nucleoplasm GO:0005654 IDA
    endoplasmic reticulum membrane GO:0005789 IBA
    endoplasmic reticulum membrane GO:0005789 TAS
    Golgi apparatus GO:0005794 IBA
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 IDA
    ER to Golgi transport vesicle membrane GO:0012507 IBA
    ER to Golgi transport vesicle membrane GO:0012507 TAS
    membrane GO:0016020 HDA
    SNARE complex GO:0031201 IBA
    SNARE complex GO:0031201 ISS
    late endosome membrane GO:0031902 IBA
    endoplasmic reticulum-Golgi intermediate compartment membrane GO:0033116 TAS
 Experiment description of studies that identified GOSR2 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for GOSR2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SEC22B 9554
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 RIT1 6016
Negative Genetic Homo sapiens
3 GJD3  
Proximity Label-MS Homo sapiens
4 HIBADH 11112
Two-hybrid Homo sapiens
5 CSGALNACT2 55454
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 TMCO5A  
Two-hybrid Homo sapiens
7 SLC10A6  
Two-hybrid Homo sapiens
8 CHRNA4  
Affinity Capture-MS Homo sapiens
9 VAPA 9218
Affinity Capture-MS Homo sapiens
10 NAPA 8775
Affinity Capture-MS Homo sapiens
11 TMEM205 374882
Two-hybrid Homo sapiens
12 MFSD5  
Two-hybrid Homo sapiens
13 HSD17B11 51170
Proximity Label-MS Homo sapiens
14 DNAJC25 548645
Proximity Label-MS Homo sapiens
15 PTPN1 5770
Proximity Label-MS Homo sapiens
16 STX4 6810
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
17 SLC30A4 7782
Affinity Capture-MS Homo sapiens
18 APP 351
Reconstituted Complex Homo sapiens
19 B3GAT1  
Proximity Label-MS Homo sapiens
20 STX1A 6804
Two-hybrid Homo sapiens
21 PELI1 57162
Two-hybrid Homo sapiens
22 FAM174A 345757
Two-hybrid Homo sapiens
23 TRDN 10345
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 STX8 9482
Affinity Capture-MS Homo sapiens
25 STX7 8417
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 GPRC5B 51704
Affinity Capture-MS Homo sapiens
27 HIATL1 84641
Two-hybrid Homo sapiens
28 PHB 5245
Co-fractionation Homo sapiens
29 BET1 10282
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 GPX8 493869
Two-hybrid Homo sapiens
31 TMEM106A  
Two-hybrid Homo sapiens
32 EBAG9 9166
Proximity Label-MS Homo sapiens
33 RIC3  
Two-hybrid Homo sapiens
34 STX12 23673
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 TMEM106C 79022
Two-hybrid Homo sapiens
36 CHST7  
Affinity Capture-MS Homo sapiens
37 SCFD1 23256
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 CX3CL1  
Affinity Capture-MS Homo sapiens
39 EMD 2010
Proximity Label-MS Homo sapiens
40 GOLGA8F  
Two-hybrid Homo sapiens
41 JAGN1 84522
Two-hybrid Homo sapiens
42 GOLGA8EP  
Two-hybrid Homo sapiens
43 BET1L 51272
Affinity Capture-MS Homo sapiens
44 KRTCAP3 200634
Affinity Capture-MS Homo sapiens
45 SFT2D3  
Affinity Capture-MS Homo sapiens
46 STX6 10228
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
47 TMEM56  
Two-hybrid Homo sapiens
48 STX5 6811
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
49 ARL13B 200894
Two-hybrid Homo sapiens
50 MFF 56947
Two-hybrid Homo sapiens
51 SEC61B 10952
Proximity Label-MS Homo sapiens
52 TMEM35  
Two-hybrid Homo sapiens
53 DKK3 27122
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 TTYH1  
Affinity Capture-MS Homo sapiens
55 BTNL9  
Affinity Capture-MS Homo sapiens
56 TM4SF19  
Two-hybrid Homo sapiens
57 MARCH8  
Two-hybrid Homo sapiens
58 HSD3B7 80270
Proximity Label-MS Homo sapiens
59 NIPAL1  
Affinity Capture-MS Homo sapiens
60 CCDC155  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
61 HSD17B13  
Two-hybrid Homo sapiens
62 LPAR1 1902
Affinity Capture-MS Homo sapiens
63 LEUTX  
Two-hybrid Homo sapiens
64 GPR152  
Two-hybrid Homo sapiens
65 FAM209A 200232
Two-hybrid Homo sapiens
66 GPR42  
Two-hybrid Homo sapiens
67 FAM134C 162427
Two-hybrid Homo sapiens
68 Uso1 56041
Affinity Capture-MS Mus musculus
69 ATP2A1 487
Proximity Label-MS Homo sapiens
70 CLEC12A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 SLC10A1  
Two-hybrid Homo sapiens
72 LIME1 54923
Two-hybrid Homo sapiens
73 FATE1  
Two-hybrid Homo sapiens
74 CREB3L1  
Two-hybrid Homo sapiens
75 USE1 55850
Affinity Capture-MS Homo sapiens
76 NSF 4905
Affinity Capture-MS Homo sapiens
77 RNF19B  
Two-hybrid Homo sapiens
78 GOSR1 9527
Affinity Capture-MS Homo sapiens
79 IER3IP1 51124
Two-hybrid Homo sapiens
80 VAMP4 8674
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 EBP  
Two-hybrid Homo sapiens
82 ERGIC1 57222
Proximity Label-MS Homo sapiens
83 MMGT1 93380
Two-hybrid Homo sapiens
84 ERGIC2 51290
Proximity Label-MS Homo sapiens
85 SEC62 7095
Proximity Label-MS Homo sapiens
86 RAB7A 7879
Proximity Label-MS Homo sapiens
87 BLCAP  
Two-hybrid Homo sapiens
88 VAMP5 10791
Affinity Capture-MS Homo sapiens
89 KRAS 3845
Negative Genetic Homo sapiens
90 ELOVL4  
Two-hybrid Homo sapiens
91 USO1 8615
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
92 DERL1 79139
Proximity Label-MS Homo sapiens
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