Gene description for NBAS
Gene name neuroblastoma amplified sequence
Gene symbol NBAS
Other names/aliases NAG
SOPH
Species Homo sapiens
 Database cross references - NBAS
ExoCarta ExoCarta_51594
Vesiclepedia VP_51594
Entrez Gene 51594
HGNC 15625
MIM 608025
UniProt A2RRP1  
 NBAS identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for NBAS
Molecular Function
    SNARE binding GO:0000149 IBA
    SNARE binding GO:0000149 IDA
    protein binding GO:0005515 IPI
Biological Process
    nuclear-transcribed mRNA catabolic process GO:0000956 IMP
    retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum GO:0006890 IBA
    retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum GO:0006890 IMP
    retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum GO:0006890 NAS
    protein transport GO:0015031 IEA
    negative regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:2000623 IMP
Subcellular Localization
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum membrane GO:0005789 IEA
    cytosol GO:0005829 TAS
    membrane GO:0016020 HDA
    Dsl1/NZR complex GO:0070939 IBA
    Dsl1/NZR complex GO:0070939 IDA
    Dsl1/NZR complex GO:0070939 IPI
 Experiment description of studies that identified NBAS in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for NBAS
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SEC22B 9554
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 Kif19a  
Affinity Capture-MS Mus musculus
3 REEP5 7905
Proximity Label-MS Homo sapiens
4 DVL2 1856
Affinity Capture-MS Homo sapiens
5 BET1 10282
Affinity Capture-MS Homo sapiens
6 GJD3  
Proximity Label-MS Homo sapiens
7 BCAP31 10134
Proximity Label-MS Homo sapiens
8 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
9 FASN 2194
Negative Genetic Homo sapiens
10 USE1 55850
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 DNAJC25 548645
Proximity Label-MS Homo sapiens
12 SCFD1 23256
Affinity Capture-MS Homo sapiens
13 VTI1B 10490
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 RPN2 6185
Proximity Label-MS Homo sapiens
15 STX12 23673
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 SCFD2 152579
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 RAB7A 7879
Proximity Label-MS Homo sapiens
18 METTL7A 25840
Proximity Label-MS Homo sapiens
19 PGRMC1 10857
Affinity Capture-MS Homo sapiens
20 LRRC59 55379
Proximity Label-MS Homo sapiens
21 GJA1 2697
Proximity Label-MS Homo sapiens
22 GOSR1 9527
Affinity Capture-MS Homo sapiens
23 NUP155 9631
Proximity Label-MS Homo sapiens
24 TEX28  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 PFN1 5216
Proximity Label-MS Homo sapiens
26 CDH5  
Affinity Capture-MS Homo sapiens
27 Zw10  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
28 TMEM9B 56674
Affinity Capture-MS Homo sapiens
29 GOLT1B 51026
Affinity Capture-MS Homo sapiens
30 EFNA5  
Affinity Capture-MS Homo sapiens
31 VAMP4 8674
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 HSD3B7 80270
Proximity Label-MS Homo sapiens
33 CUL3 8452
Affinity Capture-MS Homo sapiens
34 NAPA 8775
Affinity Capture-MS Homo sapiens
35 STIM1 6786
Proximity Label-MS Homo sapiens
36 HSD17B11 51170
Proximity Label-MS Homo sapiens
37 EMD 2010
Proximity Label-MS Homo sapiens
38 HSPB1 3315
Two-hybrid Homo sapiens
39 FKBP8 23770
Proximity Label-MS Homo sapiens
40 COPE 11316
Affinity Capture-MS Homo sapiens
41 PTPN1 5770
Proximity Label-MS Homo sapiens
42 OR1M1  
Affinity Capture-MS Homo sapiens
43 STX4 6810
Proximity Label-MS Homo sapiens
44 RPN1 6184
Proximity Label-MS Homo sapiens
45 KIAA1715 80856
Proximity Label-MS Homo sapiens
46 BNIP1 662
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 FIS1 51024
Proximity Label-MS Homo sapiens
48 C3orf52  
Affinity Capture-MS Homo sapiens
49 SEC62 7095
Proximity Label-MS Homo sapiens
50 EFNA4  
Affinity Capture-MS Homo sapiens
51 B3GAT1  
Proximity Label-MS Homo sapiens
52 NTRK1 4914
Affinity Capture-MS Homo sapiens
53 RINT1 60561
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 EGFR 1956
Negative Genetic Homo sapiens
55 RAB9A 9367
Proximity Label-MS Homo sapiens
56 C19orf25  
Affinity Capture-MS Homo sapiens
57 DHFRL1  
Proximity Label-MS Homo sapiens
58 COPB2 9276
Affinity Capture-MS Homo sapiens
59 MLNR  
Affinity Capture-MS Homo sapiens
60 DNAJC1 64215
Proximity Label-MS Homo sapiens
61 NAPG 8774
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 STX3 6809
Affinity Capture-MS Homo sapiens
63 COPA 1314
Affinity Capture-MS Homo sapiens
64 SSR1 6745
Proximity Label-MS Homo sapiens
65 STX18 53407
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 ANKRD46 157567
Affinity Capture-MS Homo sapiens
67 GPR17 2840
Affinity Capture-MS Homo sapiens
68 AK1 203
Affinity Capture-MS Homo sapiens
69 ELOVL5 60481
Proximity Label-MS Homo sapiens
70 SEC61B 10952
Proximity Label-MS Homo sapiens
71 ZDHHC12  
Affinity Capture-MS Homo sapiens
72 STX6 10228
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 DERL1 79139
Proximity Label-MS Homo sapiens
74 SLC25A46 91137
Proximity Label-MS Homo sapiens
75 VAMP5 10791
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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