Gene description for CLDN5
Gene name claudin 5
Gene symbol CLDN5
Other names/aliases AWAL
BEC1
CPETRL1
TMVCF
Species Homo sapiens
 Database cross references - CLDN5
ExoCarta ExoCarta_7122
Entrez Gene 7122
HGNC 2047
MIM 602101
UniProt O00501  
 CLDN5 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 19619303    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for CLDN5
Molecular Function
    structural molecule activity GO:0005198 IEA
    identical protein binding GO:0042802 ISS
Biological Process
    calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules GO:0016338 ISS
Subcellular Localization
    extracellular exosome GO:0070062 IDA
    integral component of membrane GO:0016021 IEA
    cell-cell junction GO:0005911 IDA
    plasma membrane GO:0005886 IEA
    bicellular tight junction GO:0005923 ISS
 Experiment description of studies that identified CLDN5 in exosomes
1
Experiment ID 67
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 19619303    
Organism Homo sapiens
Experiment description Claudin-containing exosomes in the peripheral circulation of women with ovarian cancer.
Authors Li J, Sherman-Baust CA, Tsai-Turton M, Bristow RE, Roden RB, Morin PJ
Journal name BMC_CANCER
Publication year 2009
Sample Ovarian cancer cells
Sample name BG-1
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15-1.21 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Immunoelectron Microscopy
2
Experiment ID 231
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 232
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 233
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CLDN5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CLDN3 1365
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
2 TJP1 7082
Invitro Homo sapiens
3 CLDN1 9076
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
4 MPDZ  
Invivo Homo sapiens
View the network image/svg+xml
 Pathways in which CLDN5 is involved
PathwayEvidenceSource
Tight junction interactions TAS Reactome





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