Gene description for TSPAN2
Gene name tetraspanin 2
Gene symbol TSPAN2
Other names/aliases NET3
TSN2
TSPAN-2
Species Homo sapiens
 Database cross references - TSPAN2
ExoCarta ExoCarta_10100
Vesiclepedia VP_10100
Entrez Gene 10100
HGNC 20659
MIM 613133
UniProt O60636  
 TSPAN2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for TSPAN2
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    inflammatory response GO:0006954 IEA
    astrocyte development GO:0014002 IEA
    microglia development GO:0014005 IEA
    myelination GO:0042552 IEA
    oligodendrocyte differentiation GO:0048709 IEA
    axon development GO:0061564 IEA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    plasma membrane GO:0005886 IBA
    membrane GO:0016020 TAS
    myelin sheath GO:0043209 IEA
 Experiment description of studies that identified TSPAN2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for TSPAN2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 IFNGR2  
Two-hybrid Homo sapiens
2 SNX13  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 KDELR3 11015
Affinity Capture-MS Homo sapiens
4 SLC30A8  
Two-hybrid Homo sapiens
5 SNX14  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 RAB35 11021
Affinity Capture-MS Homo sapiens
7 LCLAT1 253558
Affinity Capture-MS Homo sapiens
8 C4orf32  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 MTCH1 23787
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 SLC10A6  
Two-hybrid Homo sapiens
11 REEP5 7905
Affinity Capture-MS Homo sapiens
12 LEPROTL1 23484
Two-hybrid Homo sapiens
13 FAM167A  
Affinity Capture-MS Homo sapiens
14 TTYH3 80727
Affinity Capture-MS Homo sapiens
15 RNF19B  
Two-hybrid Homo sapiens
16 PIEZO1 9780
Affinity Capture-MS Homo sapiens
17 ARL6IP5 10550
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 LGALS3 3958
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 VKORC1L1 154807
Affinity Capture-MS Homo sapiens
20 ZFYVE27 118813
Two-hybrid Homo sapiens
21 TMEM14B  
Two-hybrid Homo sapiens
22 ATP1B4  
Two-hybrid Homo sapiens
23 TSPAN15 23555
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 ABHD16A 7920
Two-hybrid Homo sapiens
25 REEP4  
Two-hybrid Homo sapiens
26 ARF6 382
Affinity Capture-MS Homo sapiens
27 CD79A  
Two-hybrid Homo sapiens
28 LPCAT3 10162
Affinity Capture-MS Homo sapiens
29 TSPAN3 10099
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 TPP1 1200
Affinity Capture-MS Homo sapiens
31 FAM209A 200232
Two-hybrid Homo sapiens
32 CLDN5 7122
Two-hybrid Homo sapiens
33 IFITM3 10410
Affinity Capture-MS Homo sapiens
34 FOS 2353
Two-hybrid Homo sapiens
35 DAGLB 221955
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 ZDHHC6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 BSCL2  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
38 CD151 977
Affinity Capture-MS Homo sapiens
39 TMEM130  
Two-hybrid Homo sapiens
40 TMED1 11018
Affinity Capture-MS Homo sapiens
41 HSDL1 83693
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 KDSR 2531
Affinity Capture-MS Homo sapiens
43 CREB3  
Two-hybrid Homo sapiens
44 C10orf54 64115
Two-hybrid Homo sapiens
45 ABCB10 23456
Affinity Capture-MS Homo sapiens
46 TMEM31  
Two-hybrid Homo sapiens
47 SIGMAR1 10280
Affinity Capture-MS Homo sapiens
48 TSPAN31 6302
Two-hybrid Homo sapiens
49 CD63 967
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 C10orf35  
Affinity Capture-MS Homo sapiens
51 ALG11  
Affinity Capture-MS Homo sapiens
52 CLEC14A  
Two-hybrid Homo sapiens
53 SCN3B  
Two-hybrid Homo sapiens
54 CD9 928
Affinity Capture-MS Homo sapiens
55 MFF 56947
Two-hybrid Homo sapiens
56 PGRMC2 10424
Two-hybrid Homo sapiens
57 PTGFRN 5738
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 MGST3 4259
Two-hybrid Homo sapiens
59 REEP6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 CREB3L1  
Two-hybrid Homo sapiens
61 TMX2 51075
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which TSPAN2 is involved
No pathways found





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