Gene description for LPCAT3
Gene name lysophosphatidylcholine acyltransferase 3
Gene symbol LPCAT3
Other names/aliases C3F
LPCAT
LPLAT 5
LPSAT
MBOAT5
OACT5
nessy
Species Homo sapiens
 Database cross references - LPCAT3
ExoCarta ExoCarta_10162
Vesiclepedia VP_10162
Entrez Gene 10162
HGNC 30244
MIM 611950
UniProt Q6P1A2  
 LPCAT3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for LPCAT3
Molecular Function
    1-acylglycerol-3-phosphate O-acyltransferase activity GO:0003841 TAS
    2-acylglycerol-3-phosphate O-acyltransferase activity GO:0047144 TAS
    1-acylglycerophosphocholine O-acyltransferase activity GO:0047184 IBA
    1-acylglycerophosphocholine O-acyltransferase activity GO:0047184 IDA
    1-acylglycerophosphocholine O-acyltransferase activity GO:0047184 IMP
    lysophospholipid acyltransferase activity GO:0071617 IBA
    1-acylglycerophosphoethanolamine O-acyltransferase activity GO:0106262 IDA
    1-acylglycerophosphoethanolamine O-acyltransferase activity GO:0106262 ISS
    1-acylglycerophosphoserine O-acyltransferase activity GO:0106263 IDA
Biological Process
    phosphatidylcholine biosynthetic process GO:0006656 IBA
    lipid modification GO:0030258 IBA
    chylomicron assembly GO:0034378 ISS
    very-low-density lipoprotein particle assembly GO:0034379 ISS
    phosphatidylserine acyl-chain remodeling GO:0036150 IDA
    phosphatidylserine acyl-chain remodeling GO:0036150 IMP
    phosphatidylserine acyl-chain remodeling GO:0036150 TAS
    phosphatidylcholine acyl-chain remodeling GO:0036151 IDA
    phosphatidylcholine acyl-chain remodeling GO:0036151 IMP
    phosphatidylcholine acyl-chain remodeling GO:0036151 ISS
    phosphatidylcholine acyl-chain remodeling GO:0036151 TAS
    phosphatidylethanolamine acyl-chain remodeling GO:0036152 IBA
    phosphatidylethanolamine acyl-chain remodeling GO:0036152 IDA
    phosphatidylethanolamine acyl-chain remodeling GO:0036152 IMP
    phosphatidylethanolamine acyl-chain remodeling GO:0036152 TAS
    intestinal stem cell homeostasis GO:0036335 ISS
    regulation of cholesterol biosynthetic process GO:0045540 ISS
    positive regulation of intestinal cholesterol absorption GO:0045797 ISS
    negative regulation of inflammatory response GO:0050728 ISS
    endoplasmic reticulum membrane organization GO:0090158 ISS
    negative regulation of response to endoplasmic reticulum stress GO:1903573 ISS
    positive regulation of triglyceride transport GO:1905885 ISS
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 IDA
    endoplasmic reticulum membrane GO:0005789 TAS
    membrane GO:0016020 HDA
    membrane GO:0016020 IBA
 Experiment description of studies that identified LPCAT3 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 231
MISEV standards
Biophysical techniques
Alix|CD63|CD9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 232
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 233
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for LPCAT3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FBXL4 26235
Affinity Capture-MS Homo sapiens
2 CLPP 8192
Proximity Label-MS Homo sapiens
3 SLC22A16 85413
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
5 SLC22A6 9356
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 RAMP2  
Affinity Capture-MS Homo sapiens
7 TACR1  
Affinity Capture-MS Homo sapiens
8 GYPB  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 PTH1R  
Affinity Capture-MS Homo sapiens
10 CXCR4 7852
Affinity Capture-MS Homo sapiens
11 TTYH1  
Affinity Capture-MS Homo sapiens
12 GPR17 2840
Affinity Capture-MS Homo sapiens
13 ATP5J 522
Affinity Capture-MS Homo sapiens
14 KRTCAP2 200185
Affinity Capture-MS Homo sapiens
15 KDELR3 11015
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 CD63 967
Affinity Capture-MS Homo sapiens
17 C3AR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 HCST  
Affinity Capture-MS Homo sapiens
19 VTN 7448
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 LPAR6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 RPA2 6118
Proximity Label-MS Homo sapiens
22 KCNK1  
Affinity Capture-MS Homo sapiens
23 SLC26A6 65010
Affinity Capture-MS Homo sapiens
24 CRELD1 78987
Affinity Capture-MS Homo sapiens
25 CHRM4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 TSPAN15 23555
Affinity Capture-MS Homo sapiens
27 CAPZA2 830
Affinity Capture-MS Homo sapiens
28 GCGR  
Affinity Capture-MS Homo sapiens
29 PDE2A  
Affinity Capture-MS Homo sapiens
30 SLC5A6 8884
Affinity Capture-MS Homo sapiens
31 PTDSS1 9791
Affinity Capture-MS Homo sapiens
32 HTR2C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 TMEM72  
Affinity Capture-MS Homo sapiens
34 AQP3  
Affinity Capture-MS Homo sapiens
35 OPRL1 4987
Affinity Capture-MS Homo sapiens
36 PLD5  
Affinity Capture-MS Homo sapiens
37 TNFSF18 8995
Affinity Capture-MS Homo sapiens
38 LPAR1 1902
Affinity Capture-MS Homo sapiens
39 TTC30B  
Affinity Capture-MS Homo sapiens
40 KCNE1  
Affinity Capture-MS Homo sapiens
41 TSPAN2 10100
Affinity Capture-MS Homo sapiens
42 KIAA1429 25962
Affinity Capture-MS Homo sapiens
43 SLC6A20  
Affinity Capture-MS Homo sapiens
44 TSPAN8 7103
Affinity Capture-MS Homo sapiens
45 SLC5A8 160728
Affinity Capture-MS Homo sapiens
46 RPA3 6119
Proximity Label-MS Homo sapiens
47 CMTM5  
Affinity Capture-MS Homo sapiens
48 POMK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 GPR182  
Affinity Capture-MS Homo sapiens
50 SLC24A3  
Affinity Capture-MS Homo sapiens
51 PARP1 142
Proximity Label-MS Homo sapiens
52 RAB7A 7879
Proximity Label-MS Homo sapiens
53 IPPK  
Affinity Capture-MS Homo sapiens
54 OPRM1 4988
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 ATP5C1 509
Affinity Capture-MS Homo sapiens
56 PML 5371
Affinity Capture-MS Homo sapiens
57 VIPR1  
Affinity Capture-MS Homo sapiens
58 CCR6  
Affinity Capture-MS Homo sapiens
59 IL2RA  
Affinity Capture-MS Homo sapiens
60 UPK1A 11045
Affinity Capture-MS Homo sapiens
61 SLC22A4 6583
Affinity Capture-MS Homo sapiens
62 FFAR1  
Affinity Capture-MS Homo sapiens
63 CSGALNACT1 55790
Affinity Capture-MS Homo sapiens
64 MLNR  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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