Gene description for DAGLB
Gene name diacylglycerol lipase, beta
Gene symbol DAGLB
Other names/aliases DAGLBETA
KCCR13L
Species Homo sapiens
 Database cross references - DAGLB
ExoCarta ExoCarta_221955
Vesiclepedia VP_221955
Entrez Gene 221955
HGNC 28923
MIM 614016
UniProt Q8NCG7  
 DAGLB identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for DAGLB
Molecular Function
    triacylglycerol lipase activity GO:0004806 IBA
    triacylglycerol lipase activity GO:0004806 ISS
    protein binding GO:0005515 IPI
    lipase activity GO:0016298 IDA
    metal ion binding GO:0046872 IEA
    monoacylglycerol lipase activity GO:0047372 TAS
Biological Process
    prostaglandin biosynthetic process GO:0001516 ISS
    monoacylglycerol biosynthetic process GO:0006640 IEA
    neuroblast proliferation GO:0007405 IEA
    positive regulation of triglyceride catabolic process GO:0010898 ISS
    arachidonate metabolic process GO:0019369 IBA
    arachidonate metabolic process GO:0019369 IDA
    arachidonate metabolic process GO:0019369 TAS
    neurogenesis GO:0022008 IBA
    diacylglycerol catabolic process GO:0046340 IBA
    regulation of inflammatory response GO:0050727 ISS
    cannabinoid biosynthetic process GO:1901696 IEA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    lysosomal membrane GO:0005765 HDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
 Experiment description of studies that identified DAGLB in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for DAGLB
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SFT2D1 113402
Affinity Capture-MS Homo sapiens
2 SGCA  
Affinity Capture-MS Homo sapiens
3 TSPAN5 10098
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 FAF2 23197
Affinity Capture-MS Homo sapiens
5 OR10H2  
Affinity Capture-MS Homo sapiens
6 TTYH1  
Affinity Capture-MS Homo sapiens
7 SLAMF7  
Affinity Capture-MS Homo sapiens
8 RXFP1  
Affinity Capture-MS Homo sapiens
9 ICAM2 3384
Affinity Capture-MS Homo sapiens
10 PCDHGA5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 CMTM8  
Affinity Capture-MS Homo sapiens
12 TSPAN2 10100
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 ZDHHC12  
Affinity Capture-MS Homo sapiens
14 LRRC25  
Affinity Capture-MS Homo sapiens
15 LRRC55  
Affinity Capture-MS Homo sapiens
16 IGSF8 93185
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 VSIG1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 PDCD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 MANSC1 54682
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 NBPF19  
Two-hybrid Homo sapiens
21 PISD  
Two-hybrid Homo sapiens
22 CLEC14A  
Affinity Capture-MS Homo sapiens
23 PARK2  
Affinity Capture-MS Homo sapiens
24 DAGLA 747
Negative Genetic Homo sapiens
25 ENTPD7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 TSPAN3 10099
Affinity Capture-MS Homo sapiens
27 SPACA1 81833
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 VSIG4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 BTN2A1 11120
Affinity Capture-MS Homo sapiens
30 PCDHGC4  
Affinity Capture-MS Homo sapiens
31 PCDHB3  
Affinity Capture-MS Homo sapiens
32 NCR3LG1 374383
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 LPAR2 9170
Affinity Capture-MS Homo sapiens
34 APLNR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 TGFA  
Affinity Capture-MS Homo sapiens
36 IPPK  
Affinity Capture-MS Homo sapiens
37 NOTCH2NL 388677
Two-hybrid Homo sapiens
38 PCDHGB4 8641
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 ACVR1C  
Affinity Capture-MS Homo sapiens
40 SGCZ  
Affinity Capture-MS Homo sapiens
41 TPCN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 TSPAN31 6302
Affinity Capture-MS Homo sapiens
43 S1PR4  
Affinity Capture-MS Homo sapiens
44 CLP1  
Two-hybrid Homo sapiens
45 SPINT2 10653
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 PMEL 6490
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 TMEM171  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 C3orf52  
Affinity Capture-MS Homo sapiens
49 TACSTD2 4070
Affinity Capture-MS Homo sapiens
50 PCDHAC2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 EDAR  
Affinity Capture-MS Homo sapiens
52 CLRN2  
Affinity Capture-MS Homo sapiens
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