Gene description for ACSL1
Gene name acyl-CoA synthetase long-chain family member 1
Gene symbol ACSL1
Other names/aliases ACS1
FACL1
FACL2
LACS
LACS1
LACS2
Species Homo sapiens
 Database cross references - ACSL1
ExoCarta ExoCarta_2180
Vesiclepedia VP_2180
Entrez Gene 2180
HGNC 3569
MIM 152425
UniProt P33121  
 ACSL1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells Unpublished / Not applicable
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for ACSL1
Molecular Function
    long-chain fatty acid-CoA ligase activity GO:0004467 IDA
    long-chain fatty acid-CoA ligase activity GO:0004467 TAS
    ATP binding GO:0005524 IEA
    protein serine/threonine kinase activator activity GO:0043539 IEA
    arachidonate-CoA ligase activity GO:0047676 IBA
    arachidonate-CoA ligase activity GO:0047676 ISS
    phytanate-CoA ligase activity GO:0050197 ISS
    pristanate-CoA ligase activity GO:0070251 IEA
    oleoyl-CoA ligase activity GO:0090434 ISS
Biological Process
    very long-chain fatty acid metabolic process GO:0000038 IBA
    long-chain fatty acid metabolic process GO:0001676 IBA
    long-chain fatty acid metabolic process GO:0001676 IDA
    response to nutrient GO:0007584 IEA
    lipid biosynthetic process GO:0008610 IDA
    lipid biosynthetic process GO:0008610 IMP
    positive regulation of long-chain fatty acid import across plasma membrane GO:0010747 ISS
    response to organic cyclic compound GO:0014070 IEA
    fatty acid transport GO:0015908 IMP
    triglyceride biosynthetic process GO:0019432 IEA
    adiponectin-activated signaling pathway GO:0033211 IEA
    response to oleic acid GO:0034201 IEA
    long-chain fatty-acyl-CoA biosynthetic process GO:0035338 IBA
    long-chain fatty-acyl-CoA biosynthetic process GO:0035338 TAS
    xenobiotic catabolic process GO:0042178 IEA
    long-chain fatty acid import into cell GO:0044539 IDA
    long-chain fatty acid import into cell GO:0044539 ISS
    positive regulation of cold-induced thermogenesis GO:0120162 ISS
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 ISS
    mitochondrial outer membrane GO:0005741 TAS
    peroxisomal membrane GO:0005778 IEA
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum membrane GO:0005789 TAS
    membrane GO:0016020 HDA
    membrane GO:0016020 IBA
 Experiment description of studies that identified ACSL1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ACSL1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BRINP2  
Affinity Capture-MS Homo sapiens
2 SGCA  
Affinity Capture-MS Homo sapiens
3 LIMA1 51474
Co-fractionation Homo sapiens
4 C15orf48  
Affinity Capture-MS Homo sapiens
5 HLA-DPB1 3115
Affinity Capture-MS Homo sapiens
6 FASN 2194
Negative Genetic Homo sapiens
Affinity Capture-MS Homo sapiens
7 APLNR  
Affinity Capture-MS Homo sapiens
8 GPR45  
Affinity Capture-MS Homo sapiens
9 C9orf78 51759
Affinity Capture-MS Homo sapiens
10 ITLN1 55600
Affinity Capture-MS Homo sapiens
11 LDLRAD1  
Affinity Capture-MS Homo sapiens
12 ECT2 1894
Affinity Capture-MS Homo sapiens
13 MFSD4  
Affinity Capture-MS Homo sapiens
14 PLD6  
Affinity Capture-MS Homo sapiens
15 B3GNT3 10331
Affinity Capture-MS Homo sapiens
16 MGARP  
Affinity Capture-MS Homo sapiens
17 KLRK1  
Affinity Capture-MS Homo sapiens
18 NR3C1 2908
Affinity Capture-MS Homo sapiens
19 CTDP1 9150
Affinity Capture-MS Homo sapiens
20 RB1CC1 9821
Affinity Capture-MS Homo sapiens
21 PARK2  
Affinity Capture-MS Homo sapiens
22 NDUFA4 4697
Affinity Capture-MS Homo sapiens
23 FCN3 8547
Affinity Capture-MS Homo sapiens
24 CACNG7  
Affinity Capture-MS Homo sapiens
25 DDX58 23586
Affinity Capture-RNA Homo sapiens
26 FFAR1  
Affinity Capture-MS Homo sapiens
27 TMEM150A  
Affinity Capture-MS Homo sapiens
28 APEX1 328
Affinity Capture-RNA Homo sapiens
29 TPM1 7168
Co-fractionation Homo sapiens
30 TPM4 7171
Co-fractionation Homo sapiens
31 C12orf49  
Negative Genetic Homo sapiens
32 PLD3 23646
Affinity Capture-MS Homo sapiens
33 MESP2  
Affinity Capture-MS Homo sapiens
34 RPA3 6119
Proximity Label-MS Homo sapiens
35 NR3C2  
Two-hybrid Homo sapiens
36 NTRK1 4914
Affinity Capture-MS Homo sapiens
37 COQ9  
Affinity Capture-MS Homo sapiens
38 PINK1  
Affinity Capture-MS Homo sapiens
39 HLA-B 3106
Affinity Capture-MS Homo sapiens
40 GPR182  
Affinity Capture-MS Homo sapiens
41 AQP3  
Affinity Capture-MS Homo sapiens
42 FAM174A 345757
Affinity Capture-MS Homo sapiens
43 VIPR1  
Affinity Capture-MS Homo sapiens
44 PML 5371
Affinity Capture-MS Homo sapiens
45 GPR55  
Affinity Capture-MS Homo sapiens
46 ATP4A 495
Affinity Capture-MS Homo sapiens
47 KLRC1  
Affinity Capture-MS Homo sapiens
48 BDH1 622
Affinity Capture-MS Homo sapiens
49 SLC1A1 6505
Affinity Capture-MS Homo sapiens
50 P2RY8  
Affinity Capture-MS Homo sapiens
51 CCR1  
Affinity Capture-MS Homo sapiens
52 CEBPA  
Protein-peptide Homo sapiens
53 CACNG4  
Affinity Capture-MS Homo sapiens
54 SLC22A9  
Affinity Capture-MS Homo sapiens
55 TPM3 7170
Co-fractionation Homo sapiens
56 RAMP3  
Affinity Capture-MS Homo sapiens
57 C9orf72  
Affinity Capture-MS Homo sapiens
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