Gene description for HLA-F
Gene name major histocompatibility complex, class I, F
Gene symbol HLA-F
Other names/aliases CDA12
HLA-5.4
HLA-CDA12
HLAF
Species Homo sapiens
 Database cross references - HLA-F
ExoCarta ExoCarta_3134
Vesiclepedia VP_3134
Entrez Gene 3134
HGNC 4963
MIM 143110
UniProt P30511  
 HLA-F identified in exosomes derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for HLA-F
Molecular Function
    signaling receptor binding GO:0005102 IBA
    protein binding GO:0005515 IPI
    beta-2-microglobulin binding GO:0030881 IDA
    peptide antigen binding GO:0042605 IBA
    peptide antigen binding GO:0042605 IDA
    TAP1 binding GO:0046978 IPI
    TAP2 binding GO:0046979 IPI
    14-3-3 protein binding GO:0071889 IDA
Biological Process
    positive regulation of T cell mediated cytotoxicity GO:0001916 IBA
    positive regulation of T cell mediated cytotoxicity GO:0001916 IMP
    antigen processing and presentation of endogenous peptide antigen via MHC class Ib GO:0002476 IBA
    antigen processing and presentation of endogenous peptide antigen via MHC class Ib GO:0002476 IDA
    antigen processing and presentation of exogenous peptide antigen via MHC class Ib GO:0002477 IDA
    antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent GO:0002486 IBA
    negative regulation of T cell cytokine production GO:0002725 IDA
    negative regulation of natural killer cell cytokine production GO:0002728 IDA
    positive regulation of natural killer cell cytokine production GO:0002729 IDA
    immune response GO:0006955 IBA
    negative regulation of natural killer cell degranulation GO:0043322 IDA
    positive regulation of natural killer cell degranulation GO:0043323 IDA
    negative regulation of natural killer cell mediated cytotoxicity GO:0045953 IDA
Subcellular Localization
    Golgi membrane GO:0000139 IDA
    Golgi membrane GO:0000139 TAS
    extracellular space GO:0005615 IBA
    lysosomal membrane GO:0005765 IDA
    endoplasmic reticulum GO:0005783 IDA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    external side of plasma membrane GO:0009897 IBA
    cell surface GO:0009986 IDA
    ER to Golgi transport vesicle membrane GO:0012507 TAS
    membrane GO:0016020 HDA
    phagocytic vesicle membrane GO:0030670 TAS
    early endosome membrane GO:0031901 IDA
    early endosome membrane GO:0031901 TAS
    MHC class Ib protein complex GO:0032398 IDA
    MHC class I protein complex GO:0042612 IEA
    recycling endosome membrane GO:0055038 TAS
    lumenal side of endoplasmic reticulum membrane GO:0098553 TAS
 Experiment description of studies that identified HLA-F in exosomes
1
Experiment ID 363
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for HLA-F
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UPK2 7379
Affinity Capture-MS Homo sapiens
2 ADAM30 11085
Affinity Capture-MS Homo sapiens
3 SGCA  
Affinity Capture-MS Homo sapiens
4 PRG2  
Affinity Capture-MS Homo sapiens
5 GPIHBP1  
Affinity Capture-MS Homo sapiens
6 GRIA3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 KIAA1549  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 CLEC4A  
Affinity Capture-MS Homo sapiens
9 CA14 23632
Affinity Capture-MS Homo sapiens
10 TMEM106A  
Affinity Capture-MS Homo sapiens
11 IL17RB  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 ATP13A1 57130
Affinity Capture-MS Homo sapiens
13 TMEM30A 55754
Affinity Capture-MS Homo sapiens
14 HLA-B 3106
Affinity Capture-MS Homo sapiens
15 HLA-G 3135
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 SFTPC  
Affinity Capture-MS Homo sapiens
17 HLA-E 3133
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 TMPRSS11B 132724
Affinity Capture-MS Homo sapiens
19 DUOXA2  
Affinity Capture-MS Homo sapiens
20 TAZ  
Affinity Capture-MS Homo sapiens
21 CHST8 64377
Affinity Capture-MS Homo sapiens
22 CLEC2D  
Affinity Capture-MS Homo sapiens
23 CFC1  
Affinity Capture-MS Homo sapiens
24 CD1B 910
Affinity Capture-MS Homo sapiens
25 SNX19  
Affinity Capture-MS Homo sapiens
26 CD1A 909
Affinity Capture-MS Homo sapiens
27 BRINP1  
Affinity Capture-MS Homo sapiens
28 ECEL1  
Affinity Capture-MS Homo sapiens
29 CBLN4  
Affinity Capture-MS Homo sapiens
30 LY9 4063
Affinity Capture-MS Homo sapiens
31 MRAP2  
Affinity Capture-MS Homo sapiens
32 UGT1A7 54577
Affinity Capture-MS Homo sapiens
33 PRTN3 5657
Affinity Capture-MS Homo sapiens
34 CEACAM21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 IGLL5 100423062
Affinity Capture-MS Homo sapiens
36 AQP3  
Affinity Capture-MS Homo sapiens
37 ELSPBP1  
Affinity Capture-MS Homo sapiens
38 FUT3 2525
Affinity Capture-MS Homo sapiens
39 PTPRN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 CLEC12B  
Affinity Capture-MS Homo sapiens
41 OGFOD3 79701
Affinity Capture-MS Homo sapiens
42 PLTP 5360
Affinity Capture-MS Homo sapiens
43 ADAM21  
Affinity Capture-MS Homo sapiens
44 FIGF 2277
Affinity Capture-MS Homo sapiens
45 B2M 567
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 CHRNB4  
Affinity Capture-MS Homo sapiens
47 BRICD5  
Affinity Capture-MS Homo sapiens
48 HLA-DRB3 3125
Affinity Capture-MS Homo sapiens
49 HLA-DPA1  
Affinity Capture-MS Homo sapiens
50 CRLF1 9244
Affinity Capture-MS Homo sapiens
51 NAAA  
Affinity Capture-MS Homo sapiens
52 GPR45  
Affinity Capture-MS Homo sapiens
53 CHRM5  
Two-hybrid Homo sapiens
54 ITM2C 81618
Affinity Capture-MS Homo sapiens
55 CLEC2B  
Affinity Capture-MS Homo sapiens
56 GINM1 116254
Affinity Capture-MS Homo sapiens
57 LILRB1  
Reconstituted Complex Homo sapiens
58 GXYLT1 283464
Affinity Capture-MS Homo sapiens
59 CST11  
Affinity Capture-MS Homo sapiens
60 GML  
Affinity Capture-MS Homo sapiens
61 ASIC4  
Affinity Capture-MS Homo sapiens
62 KDM5C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 DNASE1L1 1774
Affinity Capture-MS Homo sapiens
64 GREM2 64388
Affinity Capture-MS Homo sapiens
65 LILRB2  
Reconstituted Complex Homo sapiens
66 LRRC37B  
Affinity Capture-MS Homo sapiens
67 ST8SIA5  
Affinity Capture-MS Homo sapiens
68 HLA-DRB1 3123
Affinity Capture-MS Homo sapiens
69 BTNL2  
Affinity Capture-MS Homo sapiens
70 SLAMF8  
Affinity Capture-MS Homo sapiens
71 SDF2L1 23753
Affinity Capture-MS Homo sapiens
72 CLEC4E  
Affinity Capture-MS Homo sapiens
73 SIRPD  
Affinity Capture-MS Homo sapiens
74 ST8SIA3  
Affinity Capture-MS Homo sapiens
75 ST3GAL3  
Affinity Capture-MS Homo sapiens
76 FUT1  
Affinity Capture-MS Homo sapiens
77 ZACN  
Affinity Capture-MS Homo sapiens
78 HLA-C 3107
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 ST8SIA4 7903
Affinity Capture-MS Homo sapiens
80 HLA-DQB1 3119
Affinity Capture-MS Homo sapiens
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