Gene description for ST3GAL6
Gene name ST3 beta-galactoside alpha-2,3-sialyltransferase 6
Gene symbol ST3GAL6
Other names/aliases SIAT10
ST3GALVI
Species Homo sapiens
 Database cross references - ST3GAL6
ExoCarta ExoCarta_10402
Vesiclepedia VP_10402
Entrez Gene 10402
HGNC 18080
MIM 607156
UniProt Q9Y274  
 ST3GAL6 identified in sEVs derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Saliva 19199708    
 Gene ontology annotations for ST3GAL6
Molecular Function
    beta-galactoside (CMP) alpha-2,3-sialyltransferase activity GO:0003836 TAS
    N-acetyllactosaminide alpha-2,3-sialyltransferase activity GO:0008118 IDA
    sialyltransferase activity GO:0008373 IBA
Biological Process
    protein glycosylation GO:0006486 IBA
    protein glycosylation GO:0006486 IDA
    glycolipid biosynthetic process GO:0009247 IBA
    glycolipid biosynthetic process GO:0009247 IDA
    oligosaccharide biosynthetic process GO:0009312 TAS
    keratan sulfate biosynthetic process GO:0018146 TAS
    cellular response to interleukin-6 GO:0071354 IEP
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    membrane GO:0016020 NAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified ST3GAL6 in sEVs
1
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for ST3GAL6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CLGN 1047
Affinity Capture-MS Homo sapiens
2 CHRNA4  
Affinity Capture-MS Homo sapiens
3 HLA-DRA 3122
Affinity Capture-MS Homo sapiens
4 PON2 5445
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 CLEC2D  
Affinity Capture-MS Homo sapiens
6 PDGFRA 5156
Affinity Capture-MS Homo sapiens
7 SPX  
Affinity Capture-MS Homo sapiens
8 NCEH1 57552
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 SLAMF1 6504
Affinity Capture-MS Homo sapiens
10 APP 351
Reconstituted Complex Homo sapiens
11 INS 3630
Affinity Capture-MS Homo sapiens
12 CHRNB2  
Affinity Capture-MS Homo sapiens
13 MSMB  
Affinity Capture-MS Homo sapiens
14 SCGB2A2  
Affinity Capture-MS Homo sapiens
15 C1orf54  
Affinity Capture-MS Homo sapiens
16 PSCA 8000
Affinity Capture-MS Homo sapiens
17 ITM2C 81618
Affinity Capture-MS Homo sapiens
18 FAM19A2 338811
Affinity Capture-MS Homo sapiens
19 KLRC1  
Affinity Capture-MS Homo sapiens
20 BAGE2  
Affinity Capture-MS Homo sapiens
21 DDX58 23586
Affinity Capture-RNA Homo sapiens
22 PTCH1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 CHRNA3 1136
Affinity Capture-MS Homo sapiens
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