Gene description for ST3GAL6
Gene name ST3 beta-galactoside alpha-2,3-sialyltransferase 6
Gene symbol ST3GAL6
Other names/aliases SIAT10
ST3GALVI
Species Homo sapiens
 Database cross references - ST3GAL6
ExoCarta ExoCarta_10402
Entrez Gene 10402
HGNC 18080
MIM 607156
UniProt Q9Y274  
 ST3GAL6 identified in exosomes derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Saliva 19199708    
 Gene ontology annotations for ST3GAL6
Molecular Function
    beta-galactoside alpha-2,3-sialyltransferase activity GO:0052798 IDA
Biological Process
    keratan sulfate biosynthetic process GO:0018146 TAS
    cellular protein modification process GO:0006464 IDA
    keratan sulfate metabolic process GO:0042339 TAS
    small molecule metabolic process GO:0044281 TAS
    glycolipid metabolic process GO:0006664 IDA
    carbohydrate metabolic process GO:0005975 TAS
    glycosaminoglycan metabolic process GO:0030203 TAS
    protein N-linked glycosylation via asparagine GO:0018279 TAS
    cellular response to interleukin-6 GO:0071354 IEP
    oligosaccharide metabolic process GO:0009311 IDA
    post-translational protein modification GO:0043687 TAS
    cellular protein metabolic process GO:0044267 TAS
    sialylation GO:0097503 IEA
    dolichol-linked oligosaccharide biosynthetic process GO:0006488 TAS
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    integral component of membrane GO:0016021 NAS
    integral component of Golgi membrane GO:0030173 IEA
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified ST3GAL6 in exosomes
1
Experiment ID 231
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 66
ISEV standards
✔
IEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for ST3GAL6
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which ST3GAL6 is involved
PathwayEvidenceSource
Keratan sulfate biosynthesis TAS Reactome
Pre-NOTCH Processing in Golgi TAS Reactome
Sialic acid metabolism TAS Reactome





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