Gene description for MGRN1
Gene name mahogunin ring finger 1, E3 ubiquitin protein ligase
Gene symbol MGRN1
Other names/aliases RNF156
Species Homo sapiens
 Database cross references - MGRN1
ExoCarta ExoCarta_23295
Vesiclepedia VP_23295
Entrez Gene 23295
HGNC 20254
MIM 607559
UniProt O60291  
 MGRN1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Melanoma cells 25950383    
Melanoma cells 25950383    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Urine 19056867    
 Gene ontology annotations for MGRN1
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 IDA
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
    ubiquitin protein ligase activity GO:0061630 IBA
Biological Process
    protein polyubiquitination GO:0000209 IEA
    protein monoubiquitination GO:0006513 IMP
    smoothened signaling pathway GO:0007224 IEA
    heart development GO:0007507 IEA
    endosome to lysosome transport GO:0008333 IBA
    endosome to lysosome transport GO:0008333 IMP
    protein ubiquitination GO:0016567 IBA
    negative regulation of cAMP-mediated signaling GO:0043951 IBA
    negative regulation of G protein-coupled receptor signaling pathway GO:0045744 IBA
    negative regulation of smoothened signaling pathway GO:0045879 ISS
    protein-containing complex assembly GO:0065003 IEA
    negative regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0106072 IDA
Subcellular Localization
    ubiquitin ligase complex GO:0000151 IEA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    early endosome GO:0005769 IBA
    early endosome GO:0005769 IDA
    endoplasmic reticulum GO:0005783 IDA
    cytosol GO:0005829 IEA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 IEA
    membrane GO:0016020 HDA
    intracellular membrane-bounded organelle GO:0043231 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified MGRN1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 254
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name MNT-1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 257
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for MGRN1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LY6H  
Affinity Capture-MS Homo sapiens
2 MEGF8 1954
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 UBE2W  
Two-hybrid Homo sapiens
4 ALPP 250
Affinity Capture-MS Homo sapiens
5 ELSPBP1  
Affinity Capture-MS Homo sapiens
6 BTNL2  
Affinity Capture-MS Homo sapiens
7 TMEM106A  
Affinity Capture-MS Homo sapiens
8 ATP1B4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 HLA-G 3135
Affinity Capture-MS Homo sapiens
10 NEDD4 4734
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
11 PTCH1  
Affinity Capture-MS Homo sapiens
12 MC2R  
Affinity Capture-Western Homo sapiens
13 CANX 821
Affinity Capture-MS Homo sapiens
14 MC1R 4157
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
15 MC4R  
Affinity Capture-Western Homo sapiens
16 DEFB109P1B  
Affinity Capture-MS Homo sapiens
17 EGLN3  
Affinity Capture-MS Homo sapiens
18 HLA-DRA 3122
Affinity Capture-MS Homo sapiens
19 TAZ  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 PRG3  
Affinity Capture-MS Homo sapiens
21 PTGES2 80142
Affinity Capture-MS Homo sapiens
22 ALPPL2 251
Affinity Capture-MS Homo sapiens
23 HTT 3064
Affinity Capture-Western Homo sapiens
24 WNT7A  
Affinity Capture-MS Homo sapiens
25 FAM19A4  
Affinity Capture-MS Homo sapiens
26 TSG101 7251
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
27 CUL3 8452
Affinity Capture-MS Homo sapiens
28 LGALS1 3956
Affinity Capture-MS Homo sapiens
29 UBE2T  
Two-hybrid Homo sapiens
30 C9orf40  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 UBE2E1  
Two-hybrid Homo sapiens
32 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
33 UBE2D4  
Two-hybrid Homo sapiens
34 APBB1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
35 SLURP1  
Affinity Capture-MS Homo sapiens
36 PDCD2 5134
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 PSG1  
Affinity Capture-MS Homo sapiens
38 DUSP6 1848
Affinity Capture-MS Homo sapiens
39 SFTPC  
Affinity Capture-MS Homo sapiens
40 UBE2G2  
Two-hybrid Homo sapiens
41 APEX1 328
Affinity Capture-RNA Homo sapiens
42 TRIM74  
Two-hybrid Homo sapiens
43 TUBA4A 7277
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
44 UBE2D2 7322
Two-hybrid Homo sapiens
45 UBE2I 7329
Two-hybrid Homo sapiens
46 SIRPD  
Affinity Capture-MS Homo sapiens
47 NMT1 4836
Affinity Capture-MS Homo sapiens
48 FBXO6 26270
Affinity Capture-MS Homo sapiens
49 SDF2L1 23753
Affinity Capture-MS Homo sapiens
50 FBXO2 26232
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 C19orf52  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 PSG3  
Affinity Capture-MS Homo sapiens
53 INTS7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 AMFR 267
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
55 MRAP  
Affinity Capture-Western Homo sapiens
56 HSDL1 83693
Affinity Capture-MS Homo sapiens
57 AP1M2 10053
Affinity Capture-MS Homo sapiens
58 HSPA4 3308
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
59 PTPRK 5796
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 UBE2J2 118424
Two-hybrid Homo sapiens
61 NMT2 9397
Affinity Capture-MS Homo sapiens
62 EDN3  
Affinity Capture-MS Homo sapiens
63 PATE1  
Affinity Capture-MS Homo sapiens
64 UBE2D3 7323
Two-hybrid Homo sapiens
65 CBLN4  
Affinity Capture-MS Homo sapiens
66 ATXN3 4287
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
67 ANKRD46 157567
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 CANT1 124583
Affinity Capture-MS Homo sapiens
69 ITCH 83737
Affinity Capture-Western Homo sapiens
70 GPAT2  
Affinity Capture-MS Homo sapiens
71 BTNL8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 ATRN 8455
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 PSG8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 UQCC1 55245
Affinity Capture-MS Homo sapiens
75 IGFL3  
Affinity Capture-MS Homo sapiens
76 UBE2D1 7321
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
77 SMPD1 6609
Affinity Capture-MS Homo sapiens
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