ExoCarta: Gene summary

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Gene description for UBE2J2
Gene name	ubiquitin-conjugating enzyme E2, J2
Gene symbol	UBE2J2
Other names/aliases	NCUBE-2 NCUBE2 PRO2121
Species	Homo sapiens
Database cross references - UBE2J2
ExoCarta	ExoCarta_118424
Entrez Gene	118424
HGNC	19268
UniProt	Q8N2K1
UBE2J2 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells	26054723
Hepatocellular carcinoma cells	26054723

Gene ontology annotations for UBE2J2

Molecular Function
	ATP binding	GO:0005524	IEA
	ubiquitin conjugating enzyme activity	GO:0061631	IDA
	ligase activity	GO:0016874	IEA
	ubiquitin protein ligase activity	GO:0061630	IBA
	ubiquitin protein ligase binding	GO:0031625	IBA
Biological Process
	response to unfolded protein	GO:0006986	IEA
	positive regulation of protein targeting to mitochondrion	GO:1903955	IMP
	ER-associated ubiquitin-dependent protein catabolic process	GO:0030433	IMP
	protein polyubiquitination	GO:0000209	IBA
Subcellular Localization
	endoplasmic reticulum membrane	GO:0005789	IEA
	integral component of membrane	GO:0016021	IEA
	ubiquitin ligase complex	GO:0000151	IPI
	cytoplasm	GO:0005737	IBA
	endoplasmic reticulum	GO:0005783	IBA

Experiment description of studies that identified UBE2J2 in exosomes

Experiment ID

235

ISEV standards
✔ EM	EV Biophysical techniques
✔ TSG101\|Alix\|HSC70\|GAPDH	EV Cytosolic markers
✘	EV Membrane markers
✔ HSP90B1	EV Negative markers
✔ qNano	EV Particle analysis

Identified molecule

mRNA

Identification method

RNA Sequencing

PubMed ID

26054723

Organism

Homo sapiens

Experiment description

Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs

Authors

He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N

Journal name

Carcinogenesis

Publication year

2015

Sample

Hepatocellular carcinoma cells

Sample name

HKCI-8

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient

Flotation density

1.13-1.19 g/mL

Molecules identified in the study

Protein
RNA

Methods used in the study

Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing