Gene description for UBE2J2
Gene name ubiquitin-conjugating enzyme E2, J2
Gene symbol UBE2J2
Other names/aliases NCUBE-2
NCUBE2
PRO2121
Species Homo sapiens
 Database cross references - UBE2J2
ExoCarta ExoCarta_118424
Vesiclepedia VP_118424
Entrez Gene 118424
HGNC 19268
UniProt Q8N2K1  
 UBE2J2 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for UBE2J2
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ubiquitin protein ligase binding GO:0031625 IPI
    ubiquitin conjugating enzyme activity GO:0061631 IBA
    ubiquitin conjugating enzyme activity GO:0061631 IDA
Biological Process
    protein polyubiquitination GO:0000209 IBA
    ubiquitin-dependent protein catabolic process GO:0006511 IMP
    response to unfolded protein GO:0006986 IEA
    ERAD pathway GO:0036503 IBA
    ERAD pathway GO:0036503 IMP
    protein K6-linked ubiquitination GO:0085020 IDA
    positive regulation of protein targeting to mitochondrion GO:1903955 HMP
Subcellular Localization
    ubiquitin ligase complex GO:0000151 IPI
    nucleus GO:0005634 IBA
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum membrane GO:0005789 TAS
    cytosol GO:0005829 TAS
 Experiment description of studies that identified UBE2J2 in exosomes
1
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 236
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
3
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for UBE2J2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 CDSN 1041
Affinity Capture-MS Homo sapiens
3 CASP14 23581
Affinity Capture-MS Homo sapiens
4 FASN 2194
Negative Genetic Homo sapiens
5 PKLR 5313
Affinity Capture-MS Homo sapiens
6 XIAP  
Two-hybrid Homo sapiens
7 HECW1  
Reconstituted Complex Homo sapiens
8 TRIM59  
Two-hybrid Homo sapiens
9 ATP13A2  
Two-hybrid Homo sapiens
10 TPK1  
Affinity Capture-MS Homo sapiens
11 MBL2 4153
Affinity Capture-MS Homo sapiens
12 TMEM14B  
Two-hybrid Homo sapiens
13 UBC 7316
Biochemical Activity Homo sapiens
14 PARK2  
Affinity Capture-Western Homo sapiens
15 KLK7 5650
Affinity Capture-MS Homo sapiens
16 ALOX12B  
Affinity Capture-MS Homo sapiens
17 TRIM25 7706
Two-hybrid Homo sapiens
18 HAL 3034
Affinity Capture-MS Homo sapiens
19 MGRN1 23295
Two-hybrid Homo sapiens
20 WWP2 11060
Reconstituted Complex Homo sapiens
21 ARG1 383
Affinity Capture-MS Homo sapiens
22 RNF10  
Two-hybrid Homo sapiens
23 ELOVL7 79993
Two-hybrid Homo sapiens
24 UBE2J2 118424
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
25 HERC4 26091
Reconstituted Complex Homo sapiens
26 TMEM129  
Affinity Capture-Western Homo sapiens
27 RBP1 5947
Two-hybrid Homo sapiens
28 RNF185  
Affinity Capture-Western Homo sapiens
29 ITCH 83737
Reconstituted Complex Homo sapiens
30 MDM2  
Reconstituted Complex Homo sapiens
31 NEDD4L 23327
Reconstituted Complex Homo sapiens
32 KPRP 448834
Affinity Capture-MS Homo sapiens
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