Gene ontology annotations for UBE2J2
Experiment description of studies that identified UBE2J2 in exosomes
1
Experiment ID
235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule
mRNA
Identification method
RNA Sequencing
PubMed ID
26054723
Organism
Homo sapiens
Experiment description
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors
"He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name
Carcinogenesis
Publication year
2015
Sample
Hepatocellular carcinoma cells
Sample name
HKCI-8
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein RNA
Methods used in the study
Western blotting Mass spectrometry RT-PCR RNA Sequencing
2
Experiment ID
236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule
mRNA
Identification method
RNA Sequencing
PubMed ID
26054723
Organism
Homo sapiens
Experiment description
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors
"He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name
Carcinogenesis
Publication year
2015
Sample
Hepatocellular carcinoma cells
Sample name
MHCC97L
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein RNA
Methods used in the study
Western blotting Mass spectrometry RT-PCR RNA Sequencing
3
Experiment ID
834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35333565
Organism
Homo sapiens
Experiment description
LAMP2A regulates the loading of proteins into exosomes
Authors
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name
Sci Adv
Publication year
2022
Sample
Retinal pigment epithelial cells
Sample name
ARPE-19
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
4
Experiment ID
835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35333565
Organism
Homo sapiens
Experiment description
LAMP2A regulates the loading of proteins into exosomes
Authors
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name
Sci Adv
Publication year
2022
Sample
Retinal pigment epithelial cells
Sample name
ARPE-19
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
Protein-protein interactions for UBE2J2
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
HNRNPH1
3187
Affinity Capture-RNA
Homo sapiens
2
CDSN
1041
Affinity Capture-MS
Homo sapiens
3
CASP14
23581
Affinity Capture-MS
Homo sapiens
4
FASN
2194
Negative Genetic
Homo sapiens
5
PKLR
5313
Affinity Capture-MS
Homo sapiens
6
XIAP
Two-hybrid
Homo sapiens
7
HECW1
Reconstituted Complex
Homo sapiens
8
TRIM59
Two-hybrid
Homo sapiens
9
ATP13A2
Two-hybrid
Homo sapiens
10
TPK1
Affinity Capture-MS
Homo sapiens
11
MBL2
4153
Affinity Capture-MS
Homo sapiens
12
TMEM14B
Two-hybrid
Homo sapiens
13
UBC
7316
Biochemical Activity
Homo sapiens
14
PARK2
Affinity Capture-Western
Homo sapiens
15
KLK7
5650
Affinity Capture-MS
Homo sapiens
16
ALOX12B
Affinity Capture-MS
Homo sapiens
17
TRIM25
7706
Two-hybrid
Homo sapiens
18
HAL
3034
Affinity Capture-MS
Homo sapiens
19
MGRN1
23295
Two-hybrid
Homo sapiens
20
WWP2
11060
Reconstituted Complex
Homo sapiens
21
ARG1
383
Affinity Capture-MS
Homo sapiens
22
RNF10
Two-hybrid
Homo sapiens
23
ELOVL7
79993
Two-hybrid
Homo sapiens
24
UBE2J2
118424
Biochemical Activity
Homo sapiens
Biochemical Activity
Homo sapiens
25
HERC4
26091
Reconstituted Complex
Homo sapiens
26
TMEM129
Affinity Capture-Western
Homo sapiens
27
RBP1
5947
Two-hybrid
Homo sapiens
28
RNF185
Affinity Capture-Western
Homo sapiens
29
ITCH
83737
Reconstituted Complex
Homo sapiens
30
MDM2
Reconstituted Complex
Homo sapiens
31
NEDD4L
23327
Reconstituted Complex
Homo sapiens
32
KPRP
448834
Affinity Capture-MS
Homo sapiens
View the network
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Pathways in which UBE2J2 is involved