Gene description for CANT1
Gene name calcium activated nucleotidase 1
Gene symbol CANT1
Other names/aliases DBQD
SCAN-1
SCAN1
SHAPY
Species Homo sapiens
 Database cross references - CANT1
ExoCarta ExoCarta_124583
Vesiclepedia VP_124583
Entrez Gene 124583
HGNC 19721
MIM 613165
UniProt Q8WVQ1  
 CANT1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for CANT1
Molecular Function
    GDP phosphatase activity GO:0004382 IBA
    GDP phosphatase activity GO:0004382 IDA
    calcium ion binding GO:0005509 IDA
    protein binding GO:0005515 IPI
    protein homodimerization activity GO:0042803 IDA
    ADP phosphatase activity GO:0043262 IDA
    UDP phosphatase activity GO:0045134 IBA
Biological Process
    proteoglycan biosynthetic process GO:0030166 IBA
    proteoglycan biosynthetic process GO:0030166 IMP
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 HMP
Subcellular Localization
    extracellular region GO:0005576 TAS
    endoplasmic reticulum membrane GO:0005789 IEA
    Golgi apparatus GO:0005794 IDA
    plasma membrane GO:0005886 IEA
    membrane GO:0016020 IDA
    Golgi cisterna membrane GO:0032580 IEA
    specific granule lumen GO:0035580 TAS
    extracellular exosome GO:0070062 HDA
    tertiary granule lumen GO:1904724 TAS
    ficolin-1-rich granule lumen GO:1904813 TAS
 Experiment description of studies that identified CANT1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CANT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CHST12 55501
Affinity Capture-MS Homo sapiens
2 DUSP22  
Affinity Capture-MS Homo sapiens
3 GJD3  
Proximity Label-MS Homo sapiens
4 C5AR1  
Affinity Capture-MS Homo sapiens
5 TMEM136  
Affinity Capture-MS Homo sapiens
6 CELSR1 9620
Affinity Capture-MS Homo sapiens
7 TOR1AIP2 163590
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 CANX 821
Affinity Capture-MS Homo sapiens
9 LAMTOR1 55004
Proximity Label-MS Homo sapiens
10 HFE  
Affinity Capture-MS Homo sapiens
11 LAMP3  
Proximity Label-MS Homo sapiens
12 GOLPH3 64083
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
13 ENTPD6  
Affinity Capture-MS Homo sapiens
14 GJA1 2697
Proximity Label-MS Homo sapiens
15 MFNG  
Affinity Capture-MS Homo sapiens
16 PXMP2  
Proximity Label-MS Homo sapiens
17 BZW2 28969
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 BRICD5  
Affinity Capture-MS Homo sapiens
19 LAMP1 3916
Proximity Label-MS Homo sapiens
20 CLRN1  
Two-hybrid Homo sapiens
21 KEAP1 9817
Negative Genetic Homo sapiens
22 ATG12  
Proximity Label-MS Homo sapiens
23 HSD3B7 80270
Proximity Label-MS Homo sapiens
24 NRSN1  
Affinity Capture-MS Homo sapiens
25 HSD17B11 51170
Proximity Label-MS Homo sapiens
26 LAMP2 3920
Proximity Label-MS Homo sapiens
27 TMEM130  
Affinity Capture-MS Homo sapiens
28 CPQ 10404
Affinity Capture-MS Homo sapiens
29 ERGIC1 57222
Proximity Label-MS Homo sapiens
30 RAB2A 5862
Proximity Label-MS Homo sapiens
31 ITGA6 3655
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 SLC39A9 55334
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 NXF1 10482
Affinity Capture-RNA Homo sapiens
34 KIAA0922  
Affinity Capture-MS Homo sapiens
35 MGRN1 23295
Affinity Capture-MS Homo sapiens
36 ATF6B  
Affinity Capture-MS Homo sapiens
37 FAM209A 200232
Two-hybrid Homo sapiens
38 ERGIC2 51290
Proximity Label-MS Homo sapiens
39 GOLPH3L 55204
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
40 LRRC55  
Affinity Capture-MS Homo sapiens
41 B3GAT1  
Proximity Label-MS Homo sapiens
42 CDH1 999
Proximity Label-MS Homo sapiens
43 SAAL1 113174
Affinity Capture-MS Homo sapiens
44 C16orf58 64755
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 ATP2A1 487
Affinity Capture-MS Homo sapiens
46 LRIG1 26018
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 EPB41L4A 64097
Affinity Capture-MS Homo sapiens
48 MSH5  
Affinity Capture-MS Homo sapiens
49 GPR182  
Affinity Capture-MS Homo sapiens
50 NCEH1 57552
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 GINM1 116254
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 MOV10 4343
Affinity Capture-RNA Homo sapiens
53 TNFRSF10C  
Affinity Capture-MS Homo sapiens
54 KIR2DL4  
Affinity Capture-MS Homo sapiens
55 LRRC32 2615
Affinity Capture-MS Homo sapiens
56 TGFBR3 7049
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 TMEM132A 54972
Affinity Capture-MS Homo sapiens
58 TMEM74  
Affinity Capture-MS Homo sapiens
59 PIGH  
Affinity Capture-MS Homo sapiens
60 GLT8D1 55830
Affinity Capture-MS Homo sapiens
61 CAV1 857
Proximity Label-MS Homo sapiens
62 ELOVL5 60481
Proximity Label-MS Homo sapiens
63 PLXNA1 5361
Affinity Capture-MS Homo sapiens
64 TMPRSS13  
Affinity Capture-MS Homo sapiens
65 ITM2B 9445
Affinity Capture-MS Homo sapiens
66 DKKL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 GALNT13 114805
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which CANT1 is involved
PathwayEvidenceSource
Immune System TAS Reactome
Innate Immune System TAS Reactome
Neutrophil degranulation TAS Reactome





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