Gene description for MAN2A2
Gene name mannosidase, alpha, class 2A, member 2
Gene symbol MAN2A2
Other names/aliases MANA2X
Species Homo sapiens
 Database cross references - MAN2A2
ExoCarta ExoCarta_4122
Vesiclepedia VP_4122
Entrez Gene 4122
HGNC 6825
MIM 600988
UniProt P49641  
 MAN2A2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for MAN2A2
Molecular Function
    alpha-mannosidase activity GO:0004559 IBA
    mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase activity GO:0004572 IEA
    protein binding GO:0005515 IPI
    hydrolase activity, hydrolyzing N-glycosyl compounds GO:0016799 IEA
    carbohydrate binding GO:0030246 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    mannose metabolic process GO:0006013 IEA
    protein glycosylation GO:0006486 IEA
    N-glycan processing GO:0006491 IBA
Subcellular Localization
    Golgi membrane GO:0000139 IBA
    Golgi membrane GO:0000139 TAS
 Experiment description of studies that identified MAN2A2 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for MAN2A2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LYZL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 LRRC37B  
Affinity Capture-MS Homo sapiens
3 LY6H  
Affinity Capture-MS Homo sapiens
4 C7orf34  
Affinity Capture-MS Homo sapiens
5 IKBKAP 8518
Two-hybrid Homo sapiens
6 MCOLN2  
Affinity Capture-MS Homo sapiens
7 BTNL2  
Affinity Capture-MS Homo sapiens
8 TMEM106A  
Affinity Capture-MS Homo sapiens
9 NAAA  
Affinity Capture-MS Homo sapiens
10 KLK15  
Affinity Capture-MS Homo sapiens
11 CEACAM8 1088
Affinity Capture-MS Homo sapiens
12 UGT1A7 54577
Affinity Capture-MS Homo sapiens
13 LMAN1L  
Affinity Capture-MS Homo sapiens
14 TMEM59 9528
Affinity Capture-MS Homo sapiens
15 DEFA1 1667
Affinity Capture-MS Homo sapiens
16 HLA-G 3135
Affinity Capture-MS Homo sapiens
17 WDR25  
Affinity Capture-MS Homo sapiens
18 DEFB109P1B  
Affinity Capture-MS Homo sapiens
19 HLA-E 3133
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 HLA-DRA 3122
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 CRLF2 64109
Affinity Capture-MS Homo sapiens
22 TAZ  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 TMEM25  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 GGH 8836
Affinity Capture-MS Homo sapiens
25 ASIC4  
Affinity Capture-MS Homo sapiens
26 CBLN4  
Affinity Capture-MS Homo sapiens
27 PLAUR 5329
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 SLC39A4 55630
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 PRG2  
Affinity Capture-MS Homo sapiens
30 MFAP4 4239
Affinity Capture-MS Homo sapiens
31 ECEL1  
Affinity Capture-MS Homo sapiens
32 FUT8 2530
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 NIPAL1  
Affinity Capture-MS Homo sapiens
34 DNAJB9 4189
Affinity Capture-MS Homo sapiens
35 MGAT4C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 GAA 2548
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 FBXO6 26270
Affinity Capture-MS Homo sapiens
38 CLEC12A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 MFNG  
Affinity Capture-MS Homo sapiens
40 NCR3  
Affinity Capture-MS Homo sapiens
41 EMR4P  
Affinity Capture-MS Homo sapiens
42 TNFSF18 8995
Affinity Capture-MS Homo sapiens
43 KLK2  
Affinity Capture-MS Homo sapiens
44 GPIHBP1  
Affinity Capture-MS Homo sapiens
45 PTCH1  
Affinity Capture-MS Homo sapiens
46 CLEC12B  
Affinity Capture-MS Homo sapiens
47 B4GALT6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 LYZL1  
Affinity Capture-MS Homo sapiens
49 HLA-DQA1 3117
Affinity Capture-MS Homo sapiens
50 C1QB 713
Affinity Capture-MS Homo sapiens
51 CNTNAP3  
Affinity Capture-MS Homo sapiens
52 SUSD4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 PBXIP1 57326
Affinity Capture-MS Homo sapiens
54 SEC62 7095
Affinity Capture-MS Homo sapiens
55 FIBIN  
Affinity Capture-MS Homo sapiens
56 CD1E  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 SCGB2A2  
Affinity Capture-MS Homo sapiens
58 C1orf54  
Affinity Capture-MS Homo sapiens
59 PSCA 8000
Affinity Capture-MS Homo sapiens
60 DHH  
Affinity Capture-MS Homo sapiens
61 GALNT7 51809
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 LUC7L2 51631
Two-hybrid Homo sapiens
63 PTPRK 5796
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 CLEC2B  
Affinity Capture-MS Homo sapiens
65 SDF2L1 23753
Affinity Capture-MS Homo sapiens
66 GIT1 28964
Two-hybrid Homo sapiens
67 DNASE1L1 1774
Affinity Capture-MS Homo sapiens
68 CST9L  
Affinity Capture-MS Homo sapiens
69 IL17RC 84818
Affinity Capture-MS Homo sapiens
70 KLRC1  
Affinity Capture-MS Homo sapiens
71 ST8SIA5  
Affinity Capture-MS Homo sapiens
72 CLGN 1047
Affinity Capture-MS Homo sapiens
73 IL5RA  
Affinity Capture-MS Homo sapiens
74 VIM 7431
Two-hybrid Homo sapiens
75 TRGV3  
Affinity Capture-MS Homo sapiens
76 TRAV20  
Affinity Capture-MS Homo sapiens
77 HLA-DPA1  
Affinity Capture-MS Homo sapiens
78 SLC9A6 10479
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 PDGFRA 5156
Affinity Capture-MS Homo sapiens
80 C1QTNF9B  
Affinity Capture-MS Homo sapiens
81 CHD3 1107
Two-hybrid Homo sapiens
82 CAPN2 824
Co-fractionation Homo sapiens
View the network image/svg+xml



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