Gene description for VAV3
Gene name vav 3 guanine nucleotide exchange factor
Gene symbol VAV3
Other names/aliases -
Species Homo sapiens
 Database cross references - VAV3
ExoCarta ExoCarta_10451
Vesiclepedia VP_10451
Entrez Gene 10451
HGNC 12659
MIM 605541
UniProt Q9UKW4  
 VAV3 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 34887515    
 Gene ontology annotations for VAV3
Molecular Function
    Rac guanyl-nucleotide exchange factor activity GO:0030676 IEA
    metal ion binding GO:0046872 IEA
    epidermal growth factor receptor binding GO:0005154 IEA
    SH3/SH2 adaptor activity GO:0005070 TAS
    guanyl-nucleotide exchange factor activity GO:0005085 TAS
    protein binding GO:0005515 IPI
    GTPase activator activity GO:0005096 TAS
Biological Process
    apoptotic signaling pathway GO:0097190 TAS
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
    vesicle fusion GO:0006906 IEA
    blood coagulation GO:0007596 TAS
    small GTPase mediated signal transduction GO:0007264 TAS
    platelet activation GO:0030168 TAS
    positive regulation of cell adhesion GO:0045785 IEA
    lamellipodium assembly GO:0030032 IEA
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    B cell receptor signaling pathway GO:0050853 IMP
    Fc-gamma receptor signaling pathway involved in phagocytosis GO:0038096 TAS
    ephrin receptor signaling pathway GO:0048013 TAS
    positive regulation of B cell proliferation GO:0030890 IMP
    vascular endothelial growth factor receptor signaling pathway GO:0048010 TAS
    cellular response to DNA damage stimulus GO:0006974 IMP
    neutrophil chemotaxis GO:0030593 IEA
    positive regulation of phosphatidylinositol 3-kinase activity GO:0043552 IEA
    positive regulation of GTPase activity GO:0043547 IEA
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    angiogenesis GO:0001525 IEA
    response to drug GO:0042493 IMP
    axon guidance GO:0007411 TAS
    innate immune response GO:0045087 TAS
    positive regulation of apoptotic process GO:0043065 TAS
    positive regulation of signal transduction GO:0009967 TAS
    integrin-mediated signaling pathway GO:0007229 IEA
Subcellular Localization
    plasma membrane GO:0005886 IEA
    cytosol GO:0005829 TAS
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified VAV3 in exosomes
1
Experiment ID 1203
MISEV standards
✔
EM
EV Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ.
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
 Protein-protein interactions for VAV3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ZYX 7791
Invitro Homo sapiens
Two-hybrid Homo sapiens
2 RAC1 5879
Reconstituted Complex Homo sapiens
3 INSR 3643
Affinity Capture-Western Homo sapiens
4 RHOA 387
Reconstituted Complex Homo sapiens
5 RHOG 391
Reconstituted Complex Homo sapiens
6 EGFR 1956
Affinity Capture-Western Homo sapiens
7 GRB2 2885
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
8 SHC1 6464
Affinity Capture-Western Homo sapiens
9 IGF1R 3480
Affinity Capture-Western Homo sapiens
10 PDGFRB 5159
Affinity Capture-Western Homo sapiens
11 ROS1  
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



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