Gene description for VAV3
Gene name vav 3 guanine nucleotide exchange factor
Gene symbol VAV3
Other names/aliases -
Species Homo sapiens
 Database cross references - VAV3
ExoCarta ExoCarta_10451
Vesiclepedia VP_10451
Entrez Gene 10451
HGNC 12659
MIM 605541
UniProt Q9UKW4  
 VAV3 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 34887515    
 Gene ontology annotations for VAV3
Molecular Function
    guanyl-nucleotide exchange factor activity GO:0005085 EXP
    guanyl-nucleotide exchange factor activity GO:0005085 IBA
    guanyl-nucleotide exchange factor activity GO:0005085 TAS
    GTPase activator activity GO:0005096 TAS
    epidermal growth factor receptor binding GO:0005154 IEA
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
Biological Process
    angiogenesis GO:0001525 IEA
    immune response-regulating cell surface receptor signaling pathway GO:0002768 IBA
    vesicle fusion GO:0006906 IEA
    DNA damage response GO:0006974 IMP
    integrin-mediated signaling pathway GO:0007229 IEA
    small GTPase-mediated signal transduction GO:0007264 IBA
    regulation of cell size GO:0008361 IGI
    regulation of cell size GO:0008361 IGI
    response to xenobiotic stimulus GO:0009410 IMP
    cell migration GO:0016477 IBA
    lamellipodium assembly GO:0030032 IEA
    platelet activation GO:0030168 TAS
    neutrophil chemotaxis GO:0030593 IEA
    positive regulation of B cell proliferation GO:0030890 IMP
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    Fc-gamma receptor signaling pathway involved in phagocytosis GO:0038096 TAS
    regulation of GTPase activity GO:0043087 IGI
    positive regulation of cell adhesion GO:0045785 IEA
    B cell receptor signaling pathway GO:0050853 IMP
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 IBA
Subcellular Localization
    immunological synapse GO:0001772 IDA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
 Experiment description of studies that identified VAV3 in exosomes
1
Experiment ID 1203
MISEV standards
EM
Biophysical techniques
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
 Protein-protein interactions for VAV3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ZNF326 284695
Affinity Capture-MS Homo sapiens
2 MCM7 4176
Affinity Capture-MS Homo sapiens
3 CBLB 868
Two-hybrid Homo sapiens
4 ERBB2 2064
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
5 RHOG 391
Reconstituted Complex Homo sapiens
6 GRB2 2885
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
7 SHC1 6464
Affinity Capture-Western Homo sapiens
8 TARDBP 23435
Affinity Capture-MS Homo sapiens
9 KIT 3815
Reconstituted Complex Homo sapiens
10 TRAF6 7189
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
11 CUL3 8452
Affinity Capture-MS Homo sapiens
12 ERBB4 2066
Reconstituted Complex Homo sapiens
13 INSR 3643
Affinity Capture-Western Homo sapiens
14 EGFR 1956
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
15 RBFOX2 23543
Affinity Capture-MS Homo sapiens
16 PDGFRB 5159
Affinity Capture-Western Homo sapiens
17 ERBB3 2065
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
18 ROS1  
Affinity Capture-Western Homo sapiens
19 TRAF3 7187
Affinity Capture-Western Homo sapiens
20 GAB1  
Reconstituted Complex Homo sapiens
21 ESR1  
Reconstituted Complex Homo sapiens
22 NOP58 51602
Affinity Capture-MS Homo sapiens
23 FIP1L1 81608
Affinity Capture-MS Homo sapiens
24 FAM76B  
Affinity Capture-MS Homo sapiens
25 TNFRSF11A  
Affinity Capture-Western Homo sapiens
26 RBM15 64783
Affinity Capture-MS Homo sapiens
27 DDX42 11325
Affinity Capture-MS Homo sapiens
28 CLCC1 23155
Affinity Capture-MS Homo sapiens
29 IGF1R 3480
Affinity Capture-Western Homo sapiens
30 RINT1 60561
Two-hybrid Homo sapiens
31 MET 4233
Reconstituted Complex Homo sapiens
32 LUC7L2 51631
Affinity Capture-MS Homo sapiens
33 ATP5C1 509
Affinity Capture-MS Homo sapiens
34 AAMDC  
Affinity Capture-MS Homo sapiens
35 AR 367
Reconstituted Complex Homo sapiens
36 BLNK  
Affinity Capture-MS Homo sapiens
37 RAC1 5879
Reconstituted Complex Homo sapiens
38 PSPC1 55269
Affinity Capture-MS Homo sapiens
39 CKAP4 10970
Affinity Capture-MS Homo sapiens
40 CCNT1  
Affinity Capture-MS Homo sapiens
41 RHOA 387
Reconstituted Complex Homo sapiens
FRET Homo sapiens
42 CA2 760
Affinity Capture-MS Homo sapiens
43 SERPINH1 871
Affinity Capture-MS Homo sapiens
44 UBA52 7311
Affinity Capture-MS Homo sapiens
45 TRAF1 7185
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which VAV3 is involved
PathwayEvidenceSource
Axon guidance TAS Reactome
Azathioprine ADME TAS Reactome
CDC42 GTPase cycle TAS Reactome
Cell death signalling via NRAGE, NRIF and NADE TAS Reactome
DAP12 interactions TAS Reactome
DAP12 signaling TAS Reactome
Death Receptor Signaling TAS Reactome
Developmental Biology TAS Reactome
Disease TAS Reactome
Disease IEA Reactome
Drug ADME TAS Reactome
EPH-ephrin mediated repulsion of cells TAS Reactome
EPH-Ephrin signaling TAS Reactome
Fc epsilon receptor (FCERI) signaling TAS Reactome
Fc epsilon receptor (FCERI) signaling IEA Reactome
FCERI mediated Ca+2 mobilization IEA Reactome
FCERI mediated Ca+2 mobilization TAS Reactome
FCERI mediated MAPK activation TAS Reactome
Fcgamma receptor (FCGR) dependent phagocytosis TAS Reactome
FCGR3A-mediated phagocytosis TAS Reactome
FCGR3A-mediated phagocytosis IEA Reactome
G alpha (12/13) signalling events TAS Reactome
GPCR downstream signalling TAS Reactome
GPVI-mediated activation cascade IEA Reactome
GPVI-mediated activation cascade TAS Reactome
Hemostasis IEA Reactome
Hemostasis TAS Reactome
Immune System TAS Reactome
Immune System IEA Reactome
Infectious disease TAS Reactome
Infectious disease IEA Reactome
Innate Immune System TAS Reactome
Innate Immune System IEA Reactome
Leishmania infection TAS Reactome
Leishmania infection IEA Reactome
Leishmania phagocytosis TAS Reactome
Leishmania phagocytosis IEA Reactome
Nervous system development TAS Reactome
NRAGE signals death through JNK TAS Reactome
p75 NTR receptor-mediated signalling TAS Reactome
Parasite infection TAS Reactome
Parasite infection IEA Reactome
Parasitic Infection Pathways TAS Reactome
Parasitic Infection Pathways IEA Reactome
Platelet activation, signaling and aggregation IEA Reactome
Platelet activation, signaling and aggregation TAS Reactome
RAC1 GTPase cycle TAS Reactome
RAC2 GTPase cycle TAS Reactome
Regulation of actin dynamics for phagocytic cup formation TAS Reactome
RHO GTPase cycle TAS Reactome
RHOA GTPase cycle TAS Reactome
RHOG GTPase cycle TAS Reactome
Signal Transduction TAS Reactome
Signal Transduction IEA Reactome
Signaling by GPCR TAS Reactome
Signaling by Receptor Tyrosine Kinases TAS Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
Signaling by Rho GTPases TAS Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 TAS Reactome
Signaling by VEGF TAS Reactome
Signaling by VEGF IEA Reactome
VEGFA-VEGFR2 Pathway TAS Reactome
VEGFA-VEGFR2 Pathway IEA Reactome
VEGFR2 mediated vascular permeability TAS Reactome





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