Gene description for FARS2
Gene name phenylalanyl-tRNA synthetase 2, mitochondrial
Gene symbol FARS2
Other names/aliases COXPD14
FARS1
HSPC320
PheRS
dJ520B18.2
Species Homo sapiens
 Database cross references - FARS2
ExoCarta ExoCarta_10667
Vesiclepedia VP_10667
Entrez Gene 10667
HGNC 21062
MIM 611592
UniProt O95363  
 FARS2 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for FARS2
Molecular Function
    tRNA binding GO:0000049 IDA
    phenylalanine-tRNA ligase activity GO:0004826 IBA
    phenylalanine-tRNA ligase activity GO:0004826 IDA
    phenylalanine-tRNA ligase activity GO:0004826 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
Biological Process
    tRNA aminoacylation for protein translation GO:0006418 TAS
    phenylalanyl-tRNA aminoacylation GO:0006432 IBA
    phenylalanyl-tRNA aminoacylation GO:0006432 IDA
    tRNA processing GO:0008033 IDA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 ISS
    mitochondrial matrix GO:0005759 TAS
 Experiment description of studies that identified FARS2 in exosomes
1
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for FARS2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MORN3  
Two-hybrid Homo sapiens
2 ISG15 9636
Affinity Capture-MS Homo sapiens
3 H1FNT  
Affinity Capture-MS Homo sapiens
4 ZNF346  
Affinity Capture-MS Homo sapiens
5 STAT5A 6776
Affinity Capture-MS Homo sapiens
6 HIST1H1D 3007
Affinity Capture-MS Homo sapiens
7 GAMT  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 SPERT  
Two-hybrid Homo sapiens
9 KRTAP10-3  
Two-hybrid Homo sapiens
10 RPS19 6223
Affinity Capture-MS Homo sapiens
11 MDFI  
Two-hybrid Homo sapiens
12 GRB10 2887
Two-hybrid Homo sapiens
13 STX11 8676
Two-hybrid Homo sapiens
14 TRIM54  
Two-hybrid Homo sapiens
15 RPS3 6188
Affinity Capture-MS Homo sapiens
16 MAN1C1 57134
Two-hybrid Homo sapiens
17 LLPH  
Affinity Capture-MS Homo sapiens
18 CHCHD2  
Affinity Capture-MS Homo sapiens
19 KRT40  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
20 RCBTB2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
21 CALCOCO2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
22 DGAT2L6  
Two-hybrid Homo sapiens
23 KRT31 3881
Two-hybrid Homo sapiens
24 MID2 11043
Two-hybrid Homo sapiens
25 USP45  
Affinity Capture-MS Homo sapiens
26 TOMM40 10452
Affinity Capture-MS Homo sapiens
27 NDUFV3 4731
Affinity Capture-MS Homo sapiens
28 ZNF438  
Two-hybrid Homo sapiens
29 FSD2 123722
Two-hybrid Homo sapiens
30 TFCP2 7024
Two-hybrid Homo sapiens
31 MYCN  
Affinity Capture-MS Homo sapiens
32 OASL 8638
Affinity Capture-MS Homo sapiens
33 RPL19 6143
Affinity Capture-MS Homo sapiens
34 APPL1 26060
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
35 LIN28A  
Affinity Capture-MS Homo sapiens
36 STK38L 23012
Co-fractionation Homo sapiens
37 MRPL38  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 BANP  
Two-hybrid Homo sapiens
39 KRT34  
Two-hybrid Homo sapiens
40 ZKSCAN8  
Affinity Capture-MS Homo sapiens
41 FHL5 9457
Two-hybrid Homo sapiens
42 ENTHD2  
Two-hybrid Homo sapiens
43 ZNF526  
Two-hybrid Homo sapiens
44 RIMBP3C  
Two-hybrid Homo sapiens
45 HIGD1C  
Two-hybrid Homo sapiens
46 RPS10 6204
Affinity Capture-MS Homo sapiens
47 YBX2 51087
Affinity Capture-MS Homo sapiens
48 PPP1R16B  
Two-hybrid Homo sapiens
49 AGTRAP 57085
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
50 HIST1H2AM 8336
Affinity Capture-MS Homo sapiens
51 TPM2 7169
Two-hybrid Homo sapiens
52 YIF1A 10897
Two-hybrid Homo sapiens
53 CYSRT1 375791
Two-hybrid Homo sapiens
54 RPL31 6160
Affinity Capture-MS Homo sapiens
55 CCDC152  
Two-hybrid Homo sapiens
56 HIST1H1E 3008
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 BAG4  
Two-hybrid Homo sapiens
58 CHST15 51363
Affinity Capture-MS Homo sapiens
59 CBX6  
Affinity Capture-MS Homo sapiens
60 HIST1H1C 3006
Affinity Capture-MS Homo sapiens
61 ENO1 2023
Affinity Capture-RNA Homo sapiens
62 BEX5  
Two-hybrid Homo sapiens
63 FGF13  
Affinity Capture-MS Homo sapiens
64 KRTAP10-7  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
65 MESDC2 23184
Two-hybrid Homo sapiens
66 WDR46  
Affinity Capture-MS Homo sapiens
67 DPH2 1802
Two-hybrid Homo sapiens
68 NOTCH2NL 388677
Two-hybrid Homo sapiens
69 ZNF500  
Two-hybrid Homo sapiens
70 RPS2 6187
Affinity Capture-MS Homo sapiens
71 RC3H2  
Co-fractionation Homo sapiens
72 IKZF3  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
73 KRTAP10-8  
Two-hybrid Homo sapiens
74 NFKBID  
Two-hybrid Homo sapiens
75 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 EGFR 1956
Negative Genetic Homo sapiens
77 FAHD1 81889
Affinity Capture-MS Homo sapiens
78 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 HMBOX1  
Two-hybrid Homo sapiens
80 KCNF1  
Two-hybrid Homo sapiens
81 NXF1 10482
Affinity Capture-RNA Homo sapiens
82 DVL3 1857
Two-hybrid Homo sapiens
83 SYP  
Two-hybrid Homo sapiens
84 ZNF655  
Two-hybrid Homo sapiens
85 PRR3  
Affinity Capture-MS Homo sapiens
86 TADA2A  
Two-hybrid Homo sapiens
87 CMTM5  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
88 SPRY1 10252
Two-hybrid Homo sapiens
89 TRIM27  
Two-hybrid Homo sapiens
90 CDCA4 55038
Two-hybrid Homo sapiens
91 HIST1H1A 3024
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 ZNF490  
Affinity Capture-MS Homo sapiens
93 CCDC140  
Affinity Capture-MS Homo sapiens
94 HIST1H1T 3010
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
95 RPL26 6154
Affinity Capture-MS Homo sapiens
96 ZC3H3  
Affinity Capture-MS Homo sapiens
97 MRPL18 29074
Affinity Capture-MS Homo sapiens
98 KRTAP10-5  
Two-hybrid Homo sapiens
99 ISY1-RAB43 100534599
Two-hybrid Homo sapiens
100 RPS16 6217
Affinity Capture-MS Homo sapiens
101 BACH2 60468
Two-hybrid Homo sapiens
102 KRTAP3-3  
Two-hybrid Homo sapiens
103 YBX1 4904
Affinity Capture-MS Homo sapiens
104 SURF6  
Affinity Capture-MS Homo sapiens
105 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
106 TRAF2 7186
Two-hybrid Homo sapiens
107 RRS1 23212
Affinity Capture-MS Homo sapiens
108 HOMEZ  
Two-hybrid Homo sapiens
109 ICT1 3396
Affinity Capture-MS Homo sapiens
110 ZCRB1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
111 AJUBA  
Two-hybrid Homo sapiens
112 MKRN3  
Two-hybrid Homo sapiens
113 UTP23  
Affinity Capture-MS Homo sapiens
114 KRTAP1-1  
Two-hybrid Homo sapiens
115 G3BP2 9908
Affinity Capture-MS Homo sapiens
116 SHMT2 6472
Affinity Capture-RNA Homo sapiens
117 HIST1H1B 3009
Affinity Capture-MS Homo sapiens
118 NPPB  
Affinity Capture-MS Homo sapiens
119 ADAMTSL4  
Two-hybrid Homo sapiens
120 NEIL1  
Affinity Capture-MS Homo sapiens
121 NEDD1 121441
Affinity Capture-MS Homo sapiens
122 GLI4  
Affinity Capture-MS Homo sapiens
123 RPS6 6194
Affinity Capture-MS Homo sapiens
124 KRT13 3860
Two-hybrid Homo sapiens
125 KRAS 3845
Negative Genetic Homo sapiens
126 KRTAP10-9  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which FARS2 is involved
PathwayEvidenceSource
Metabolism of proteins TAS Reactome
Mitochondrial tRNA aminoacylation TAS Reactome
Translation TAS Reactome
tRNA Aminoacylation TAS Reactome





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