Gene description for APPL1
Gene name adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1
Gene symbol APPL1
Other names/aliases APPL
DIP13alpha
Species Homo sapiens
 Database cross references - APPL1
ExoCarta ExoCarta_26060
Entrez Gene 26060
HGNC 24035
MIM 604299
UniProt Q9UKG1  
 APPL1 identified in exosomes derived from the following tissue/cell type
Urine 19056867    
 Gene ontology annotations for APPL1
Molecular Function
    protein binding GO:0005515 IPI
    protein kinase B binding GO:0043422 IPI
    identical protein binding GO:0042802 IPI
Biological Process
    insulin receptor signaling pathway GO:0008286 TAS
    programmed cell death GO:0012501 TAS
    cell proliferation GO:0008283 IDA
    regulation of establishment of protein localization to plasma membrane GO:0090003 IMP
    cell cycle GO:0007049 IEA
    extrinsic apoptotic signaling pathway GO:0097191 TAS
    signal transduction GO:0007165 TAS
    extrinsic apoptotic signaling pathway in absence of ligand GO:0097192 TAS
    regulation of glucose import GO:0046324 IMP
    apoptotic process GO:0006915 TAS
Subcellular Localization
    cytosol GO:0005829 IDA
    NuRD complex GO:0016581 IDA
    cytoplasm GO:0005737 IC
    early endosome membrane GO:0031901 IEA
    nucleus GO:0005634 IDA
    vesicle membrane GO:0012506 IDA
    endosome membrane GO:0010008 TAS
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified APPL1 in exosomes
1
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
AQP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for APPL1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DCC  
Invivo Homo sapiens
Two-hybrid Homo sapiens
2 TRAF2  
Two-hybrid Homo sapiens
3 APPL2 55198
Two-hybrid Homo sapiens
4 AKT2 208
Invitro Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which APPL1 is involved
PathwayEvidenceSource
Ligand-independent caspase activation via DCC TAS Reactome





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