Gene description for ATP5D
Gene name ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit
Gene symbol ATP5D
Other names/aliases -
Species Homo sapiens
 Database cross references - ATP5D
ExoCarta ExoCarta_513
Entrez Gene 513
HGNC 837
MIM 603150
UniProt P30049  
 ATP5D identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for ATP5D
Molecular Function
    proton-transporting ATPase activity, rotational mechanism GO:0046961 IEA
    ATP binding GO:0005524 NAS
    ADP binding GO:0043531 NAS
    transmembrane transporter activity GO:0022857 IC
    ATPase activity GO:0016887 IDA
    transporter activity GO:0005215 NAS
    proton-transporting ATP synthase activity, rotational mechanism GO:0046933 IBA
Biological Process
    cellular metabolic process GO:0044237 TAS
    response to copper ion GO:0046688 NAS
    small molecule metabolic process GO:0044281 TAS
    respiratory electron transport chain GO:0022904 TAS
    mitochondrial ATP synthesis coupled proton transport GO:0042776 TAS
    oxidative phosphorylation GO:0006119 NAS
    ATP biosynthetic process GO:0006754 NAS
    ATP synthesis coupled proton transport GO:0015986 IBA
Subcellular Localization
    mitochondrial proton-transporting ATP synthase complex, catalytic core F(1) GO:0000275 NAS
    mitochondrial inner membrane GO:0005743 ISS
    mitochondrial proton-transporting ATP synthase complex GO:0005753 NAS
    mitochondrion GO:0005739 NAS
    mitochondrial matrix GO:0005759 TAS
 Experiment description of studies that identified ATP5D in exosomes
1
Experiment ID 234
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 236
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
3
Experiment ID 191
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD81|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ATP5D
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which ATP5D is involved
PathwayEvidenceSource
Formation of ATP by chemiosmotic coupling TAS Reactome





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