Gene description for EXOSC3
Gene name exosome component 3
Gene symbol EXOSC3
Other names/aliases CGI-102
PCH1B
RRP40
Rrp40p
bA3J10.7
hRrp-40
p10
Species Homo sapiens
 Database cross references - EXOSC3
ExoCarta ExoCarta_51010
Vesiclepedia VP_51010
Entrez Gene 51010
HGNC 17944
MIM 606489
UniProt Q9NQT5  
 EXOSC3 identified in exosomes derived from the following tissue/cell type
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for EXOSC3
Molecular Function
    RNA binding GO:0003723 IBA
    3'-5'-exoribonuclease activity GO:0000175 NAS
    exoribonuclease activity GO:0004532 IDA
    protein binding GO:0005515 IPI
Biological Process
    positive regulation of isotype switching GO:0045830 IEA
    isotype switching GO:0045190 ISS
    nuclear retention of pre-mRNA with aberrant 3'-ends at the site of transcription GO:0071049 IBA
    nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5' GO:0034427 IBA
    U4 snRNA 3'-end processing GO:0034475 IBA
    exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay GO:0043928 TAS
    gene expression GO:0010467 TAS
    nuclear polyadenylation-dependent tRNA catabolic process GO:0071038 IBA
    exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) GO:0000467 IBA
    rRNA processing GO:0006364 IDA
    CUT catabolic process GO:0071034 IMP
    nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay GO:0000288 TAS
    DNA deamination GO:0045006 IDA
    polyadenylation-dependent snoRNA 3'-end processing GO:0071051 IBA
    nuclear polyadenylation-dependent rRNA catabolic process GO:0071035 IBA
Subcellular Localization
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
    nucleolus GO:0005730 IDA
    cytosol GO:0005829 TAS
    exosome (RNase complex) GO:0000178 IDA
    cytoplasmic exosome (RNase complex) GO:0000177 IDA
    nuclear exosome (RNase complex) GO:0000176 IDA
    transcriptionally active chromatin GO:0035327 IMP
 Experiment description of studies that identified EXOSC3 in exosomes
1
Experiment ID 191
MISEV standards
✘ EV Biophysical techniques
✔
Alix|CD81|CD9
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 217
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|CD81|CD9|CD63
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for EXOSC3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDK5RAP1  
Affinity Capture-MS Homo sapiens
2 EXOSC4 54512
Affinity Capture-MS Homo sapiens
3 EXOSC5 56915
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here