Gene description for NDUFB7
Gene name NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7, 18kDa
Gene symbol NDUFB7
Other names/aliases B18
CI-B18
Species Homo sapiens
 Database cross references - NDUFB7
ExoCarta ExoCarta_4713
Vesiclepedia VP_4713
Entrez Gene 4713
HGNC 7702
MIM 603842
UniProt P17568  
 NDUFB7 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for NDUFB7
Molecular Function
    protein binding GO:0005515 IPI
    NADH dehydrogenase (ubiquinone) activity GO:0008137 IMP
    NADH dehydrogenase (ubiquinone) activity GO:0008137 NAS
Biological Process
    mitochondrial electron transport, NADH to ubiquinone GO:0006120 NAS
    aerobic respiration GO:0009060 NAS
    mitochondrial respiratory chain complex I assembly GO:0032981 IMP
    proton motive force-driven mitochondrial ATP synthesis GO:0042776 NAS
    proton transmembrane transport GO:1902600 IEA
Subcellular Localization
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 HTP
    mitochondrial inner membrane GO:0005743 IDA
    mitochondrial inner membrane GO:0005743 TAS
    mitochondrial intermembrane space GO:0005758 IDA
    respiratory chain complex I GO:0045271 IBA
    respiratory chain complex I GO:0045271 IDA
 Experiment description of studies that identified NDUFB7 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 234
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
7
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for NDUFB7
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NDUFV1 4723
Affinity Capture-MS Homo sapiens
2 CRELD1 78987
Affinity Capture-MS Homo sapiens
3 Tmed2 56334
Affinity Capture-MS Mus musculus
4 FASN 2194
Positive Genetic Homo sapiens
5 UQCRQ 27089
Co-fractionation Homo sapiens
6 SLC25A12 8604
Proximity Label-MS Homo sapiens
7 MAK  
Two-hybrid Homo sapiens
8 NDUFS5 4725
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 NDUFA1 4694
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 NDUFB10 4716
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
11 NDUFA9 4704
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 ICT1 3396
Co-fractionation Homo sapiens
13 ND5 4540
Affinity Capture-MS Homo sapiens
14 UQCRC2 7385
Co-fractionation Homo sapiens
15 MPV17 4358
Co-fractionation Homo sapiens
16 NDUFB5 4711
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 HSPA1L 3305
Co-fractionation Homo sapiens
18 NDUFS4 4724
Affinity Capture-MS Homo sapiens
19 ATP5F1 515
Co-fractionation Homo sapiens
20 NDUFA12 55967
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 NDUFB1 4707
Affinity Capture-MS Homo sapiens
22 CHCHD4  
Affinity Capture-MS Homo sapiens
23 NDUFA4 4697
Affinity Capture-MS Homo sapiens
24 ATP1B3 483
Co-fractionation Homo sapiens
25 NDUFA8 4702
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 MMGT1 93380
Affinity Capture-MS Homo sapiens
27 NDUFS6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 NDUFS7 374291
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 NDUFB9 4715
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
30 METAP2 10988
Affinity Capture-MS Homo sapiens
31 CKAP4 10970
Two-hybrid Homo sapiens
32 NDUFS8 4728
Affinity Capture-MS Homo sapiens
33 NDUFS3 4722
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 NDUFB3 4709
Affinity Capture-MS Homo sapiens
35 ATP6V0D1 9114
Co-fractionation Homo sapiens
36 ND1  
Affinity Capture-MS Homo sapiens
37 NDUFB8 4714
Affinity Capture-MS Homo sapiens
38 RAB7A 7879
Affinity Capture-MS Homo sapiens
39 PHB2 11331
Co-fractionation Homo sapiens
40 NDUFA7 4701
Affinity Capture-MS Homo sapiens
41 NDUFB6 4712
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
42 NDUFA2 4695
Co-fractionation Homo sapiens
43 ZMPSTE24 10269
Co-fractionation Homo sapiens
44 RAB5C 5878
Affinity Capture-MS Homo sapiens
45 NDUFB11 54539
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 NDUFA10 4705
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
47 TOMM7 54543
Co-fractionation Homo sapiens
48 VDAC3 7419
Co-fractionation Homo sapiens
49 CD55 1604
Co-fractionation Homo sapiens
50 DNAJC30  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 NDUFV2 4729
Affinity Capture-MS Homo sapiens
52 NDUFC2 4718
Affinity Capture-MS Homo sapiens
53 NDUFV3 4731
Affinity Capture-MS Homo sapiens
54 ND4 4538
Affinity Capture-MS Homo sapiens
55 LAMTOR3 8649
Co-fractionation Homo sapiens
56 COX4I1 1327
Co-fractionation Homo sapiens
57 GOLT1B 51026
Affinity Capture-MS Homo sapiens
58 MLKL 197259
Two-hybrid Homo sapiens
View the network image/svg+xml



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