ExoCarta: Gene summary

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Gene description for MRPL49
Gene name	mitochondrial ribosomal protein L49
Gene symbol	MRPL49
Other names/aliases	C11orf4 L49mt MRP-L49 NOF NOF1
Species	Homo sapiens
Database cross references - MRPL49
ExoCarta	ExoCarta_740
Vesiclepedia	VP_740
Entrez Gene	740
HGNC	1176
MIM	606866
UniProt	Q13405
MRPL49 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942

Gene ontology annotations for MRPL49

Molecular Function
	structural constituent of ribosome	GO:0003735	IBA
	protein binding	GO:0005515	IPI
Biological Process
	mitochondrial translation	GO:0032543	NAS
Subcellular Localization
	mitochondrion	GO:0005739	HTP
	mitochondrion	GO:0005739	IDA
	mitochondrial inner membrane	GO:0005743	NAS
	mitochondrial inner membrane	GO:0005743	TAS
	mitochondrial ribosome	GO:0005761	IDA
	mitochondrial large ribosomal subunit	GO:0005762	IBA
	mitochondrial large ribosomal subunit	GO:0005762	IDA
	mitochondrial large ribosomal subunit	GO:0005762	NAS

Experiment description of studies that identified MRPL49 in exosomes

Experiment ID

489

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 6

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

490

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 7

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

491

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 8

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

492

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 9

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Protein-protein interactions for MRPL49

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

UBE2H

7328

Affinity Capture-MS

Homo sapiens

MIS12

Affinity Capture-MS

Homo sapiens

SLIRP

81892

Proximity Label-MS

Homo sapiens

H1FNT

Affinity Capture-MS

Homo sapiens

MRPL30

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

MRPL17

Affinity Capture-MS

Homo sapiens

TRUB2

Proximity Label-MS

Homo sapiens

MTG2

Proximity Label-MS

Homo sapiens

NGRN

Proximity Label-MS

Homo sapiens

HRAS

3265

Affinity Capture-MS

Homo sapiens

CHCHD1

Proximity Label-MS

Homo sapiens

MRPL37

51253

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

MRPL27

51264

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

NENF

29937

Co-fractionation

Homo sapiens

CAND1

55832

Affinity Capture-MS

Homo sapiens

MRPL52

Affinity Capture-MS		Homo sapiens
Co-fractionation		Homo sapiens

RPUSD4

84881

Proximity Label-MS

Homo sapiens

DNAJC15

Proximity Label-MS

Homo sapiens

MTRF1

Proximity Label-MS

Homo sapiens

APP

351

Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens

Ecd

Affinity Capture-MS

Mus musculus

LRPPRC

10128

Proximity Label-MS

Homo sapiens

Spire2

Affinity Capture-MS

Mus musculus

MCU

90550

Proximity Label-MS

Homo sapiens

PRKCDBP

112464

Co-fractionation

Homo sapiens

PMPCA

23203

Proximity Label-MS

Homo sapiens

MCM2

4171

Affinity Capture-MS

Homo sapiens

MRPL23

6150

Affinity Capture-MS

Homo sapiens

CDH2

1000

Co-fractionation

Homo sapiens

COPS5

10987

Affinity Capture-MS

Homo sapiens

TRA2B

6434

Affinity Capture-MS

Homo sapiens

HINT2

84681

Proximity Label-MS

Homo sapiens

MRPL12

6182

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

ZFYVE1

53349

Affinity Capture-MS

Homo sapiens

RPS9

6203

Affinity Capture-MS

Homo sapiens

MRPS26

64949

Proximity Label-MS

Homo sapiens

SURF1

Proximity Label-MS

Homo sapiens

EXD2

Proximity Label-MS

Homo sapiens

MRPL39

54148

Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

CEP57

Affinity Capture-MS

Homo sapiens

NHP2

55651

Affinity Capture-MS

Homo sapiens

METTL18

Affinity Capture-MS

Homo sapiens

CUL1

8454

Affinity Capture-MS

Homo sapiens

BTRC

8945

Affinity Capture-MS

Homo sapiens

PMPCB

9512

Proximity Label-MS

Homo sapiens

MRPL4

51073

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

FASTKD2

Proximity Label-MS

Homo sapiens

VWA8

23078

Proximity Label-MS

Homo sapiens

MRPL47

57129

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

RPL19

6143

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

MTERF3

Proximity Label-MS

Homo sapiens

MDH2

4191

Proximity Label-MS

Homo sapiens

MRPS30

10884

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

MRPL13

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

SCFD1

23256

Co-fractionation

Homo sapiens

C6orf203

Proximity Label-MS

Homo sapiens

THEM4

Affinity Capture-MS

Homo sapiens

COX15

1355

Affinity Capture-MS

Homo sapiens

FASTKD5

Proximity Label-MS

Homo sapiens

MRPL45

84311

Affinity Capture-MS

Homo sapiens

RPL26

6154

Affinity Capture-MS

Homo sapiens

TMEM177

Co-fractionation

Homo sapiens

SRSF3

6428

Affinity Capture-MS

Homo sapiens

YBX2

51087

Affinity Capture-MS

Homo sapiens

PPT2

Co-fractionation

Homo sapiens

TUFM

7284

Proximity Label-MS

Homo sapiens

MTIF3

Proximity Label-MS

Homo sapiens

HSCB

150274

Proximity Label-MS

Homo sapiens

RBMS2

5939

Affinity Capture-MS

Homo sapiens

CCDC109B

55013

Proximity Label-MS

Homo sapiens

MOV10

4343

Affinity Capture-RNA

Homo sapiens

RPL27A

6157

Affinity Capture-MS

Homo sapiens

MTFMT

Proximity Label-MS

Homo sapiens

MRPL11

65003

Proximity Label-MS

Homo sapiens

MTRF1L

Proximity Label-MS

Homo sapiens

TMEM70

Proximity Label-MS

Homo sapiens

CYLD

Affinity Capture-MS

Homo sapiens

MRPL2

51069

Affinity Capture-MS

Homo sapiens

MRPL41

64975

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

Brwd3

Affinity Capture-MS

Mus musculus

OXA1L

Reconstituted Complex

Homo sapiens

MRPL44

Affinity Capture-MS

Homo sapiens

MRPL51

51258

Affinity Capture-MS

Homo sapiens

MRPL21

219927

Affinity Capture-MS

Homo sapiens

MRRF

Proximity Label-MS

Homo sapiens

MRPS12

Proximity Label-MS

Homo sapiens

MTIF2

4528

Proximity Label-MS

Homo sapiens

MRPL35

Affinity Capture-MS

Homo sapiens

MRPL10

124995

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

MRPL9

65005

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

RPL37

6167

Affinity Capture-MS

Homo sapiens

PRKCZ

5590

Affinity Capture-MS

Homo sapiens

CUL3

8452

Affinity Capture-MS

Homo sapiens

TCF3

Affinity Capture-MS

Homo sapiens

MECP2

4204

Affinity Capture-MS

Homo sapiens

GADD45GIP1

Affinity Capture-MS

Homo sapiens

MRPL50

54534

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

NXF1

10482

Affinity Capture-RNA

Homo sapiens

MRPL15

29088

Affinity Capture-MS

Homo sapiens

100

MRPL1

Affinity Capture-MS

Homo sapiens

101

MYCN

Affinity Capture-MS

Homo sapiens

102

TACO1

Proximity Label-MS

Homo sapiens

103

MTG1

Proximity Label-MS

Homo sapiens

104

MRPL38

Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens

105

MYC

Affinity Capture-MS

Homo sapiens

106

DLST

1743

Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens

107

ACAD9

28976

Proximity Label-MS

Homo sapiens

108

NANOG

Affinity Capture-MS

Homo sapiens

109

METTL15

Proximity Label-MS

Homo sapiens

110

MRPL18

29074

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

111

Fbxw11

Affinity Capture-MS

Mus musculus

112

SOX2

Affinity Capture-MS

Homo sapiens

113

GFM2

84340

Proximity Label-MS

Homo sapiens

114

YBX1

4904

Affinity Capture-MS

Homo sapiens

115

1431

Proximity Label-MS

Homo sapiens

116

MRPL42

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

117

PLEKHA4

57664

Affinity Capture-MS

Homo sapiens

118

FBXW11

Affinity Capture-MS

Homo sapiens

119

TSFM

10102

Proximity Label-MS

Homo sapiens

120

AUH

549

Proximity Label-MS

Homo sapiens

121

KIAA1598

57698

Affinity Capture-MS

Homo sapiens

122

TBRG4

9238

Proximity Label-MS

Homo sapiens

123

DNAJC19

131118

Proximity Label-MS

Homo sapiens

124

Osgep

Affinity Capture-MS

Mus musculus

125

RPL6

6128

Affinity Capture-MS

Homo sapiens

126

ICT1

3396

Affinity Capture-MS		Homo sapiens
Proximity Label-MS		Homo sapiens

127

MRPL53

Affinity Capture-MS

Homo sapiens

128

TIMM10

Co-fractionation

Homo sapiens

129

DNAJA3

9093

Proximity Label-MS

Homo sapiens

130

C12orf65

Proximity Label-MS

Homo sapiens

131

MTHFD1

4522

Co-fractionation

Homo sapiens

132

C21orf33

Proximity Label-MS

Homo sapiens

133

SSBP1

6742

Proximity Label-MS

Homo sapiens

134

KLF12

Affinity Capture-MS

Homo sapiens

135

FASTKD3

Proximity Label-MS

Homo sapiens

136

GFM1

85476

Proximity Label-MS

Homo sapiens

137

MGST3

4259

Co-fractionation

Homo sapiens

138

MRPL46

Affinity Capture-MS

Homo sapiens

139

RPUSD3

Proximity Label-MS

Homo sapiens

140

MRPL19

9801

Affinity Capture-MS

Homo sapiens

141

RPS8

6202

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

142

HNRNPA1L2

144983

Affinity Capture-MS

Homo sapiens

143

GRSF1

2926

Proximity Label-MS

Homo sapiens

144

C1QBP

708

Proximity Label-MS

Homo sapiens

145

HNRNPU

3192

Affinity Capture-MS

Homo sapiens

146

C17orf80

55028

Proximity Label-MS

Homo sapiens

147

METTL17

Proximity Label-MS

Homo sapiens

148

MRPL28

10573

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

149

MRPS18A

Affinity Capture-MS

Homo sapiens

150

DDX28

Proximity Label-MS

Homo sapiens

151

RPL17

6139

Affinity Capture-MS

Homo sapiens

View the network

Pathways in which MRPL49 is involved

Pathway	Evidence	Source
Metabolism of proteins	TAS	Reactome
Metabolism of proteins	IEA	Reactome
Mitochondrial translation	TAS	Reactome
Mitochondrial translation	IEA	Reactome
Mitochondrial translation elongation	TAS	Reactome
Mitochondrial translation elongation	IEA	Reactome
Mitochondrial translation initiation	IEA	Reactome
Mitochondrial translation termination	TAS	Reactome
Translation	TAS	Reactome
Translation	IEA	Reactome

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