Gene description for CENPJ
Gene name centromere protein J
Gene symbol CENPJ
Other names/aliases BM032
CENP-J
CPAP
LAP
LIP1
MCPH6
SASS4
SCKL4
Sas-4
Species Homo sapiens
 Database cross references - CENPJ
ExoCarta ExoCarta_55835
Vesiclepedia VP_55835
Entrez Gene 55835
HGNC 17272
MIM 609279
UniProt Q9HC77  
 CENPJ identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for CENPJ
Molecular Function
    tubulin binding GO:0015631 IDA
    protein domain specific binding GO:0019904 IPI
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 IPI
Biological Process
    microtubule polymerization GO:0046785 IMP
    centriole replication GO:0007099 IMP
    microtubule nucleation GO:0007020 TAS
    organelle organization GO:0006996 TAS
    mitotic cell cycle GO:0000278 TAS
    G2/M transition of mitotic cell cycle GO:0000086 TAS
    regulation of centriole replication GO:0046599 IMP
    cell division GO:0051301 NAS
Subcellular Localization
    microtubule GO:0005874 IEA
    cytosol GO:0005829 TAS
    gamma-tubulin small complex GO:0008275 NAS
    centrosome GO:0005813 IDA
    centriole GO:0005814 IDA
 Experiment description of studies that identified CENPJ in exosomes
1
Experiment ID 834
MISEV standards
✔
EM
EV Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for CENPJ
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 STAT5A 6776
Invivo Homo sapiens
Two-hybrid Homo sapiens
2 TUBG1 7283
Invivo Homo sapiens
3 EPB41 2035
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
4 STAT5B 6777
Invivo Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml



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