Gene description for Vim
Gene name vimentin
Gene symbol Vim
Other names/aliases -
Species Mus musculus
 Database cross references - Vim
ExoCarta ExoCarta_22352
Entrez Gene 22352
UniProt P20152  
 Vim identified in exosomes derived from the following tissue/cell type
Fibroblasts 23260141    
Mov neuroglial cells 15210972    
Neural stem cells 25242146    
 Gene ontology annotations for Vim
Molecular Function
    structural molecule activity GO:0005198 IMP
    structural constituent of cytoskeleton GO:0005200 ISO
    keratin filament binding GO:1990254 ISO
    protein kinase binding GO:0019901 ISO
    identical protein binding GO:0042802 ISO
    double-stranded RNA binding GO:0003725 ISO
    protein binding GO:0005515 IPI
    kinase binding GO:0019900 ISO
    glycoprotein binding GO:0001948 ISO
    structural constituent of eye lens GO:0005212 IDA
    protein phosphatase 2A binding GO:0051721 ISO
    scaffold protein binding GO:0097110 ISO
    protein C-terminus binding GO:0008022 ISO
Biological Process
    intermediate filament-based process GO:0045103 IMP
    SMAD protein signal transduction GO:0060395 IDA
    astrocyte development GO:0014002 IGI
    regulation of Schwann cell migration GO:1900147 ISO
    lens fiber cell development GO:0070307 IDA
    negative regulation of neuron projection development GO:0010977 IGI
    intermediate filament organization GO:0045109 IGI
    positive regulation of glial cell proliferation GO:0060252 ISO
    regulation of axonogenesis GO:0050770 ISO
    Bergmann glial cell differentiation GO:0060020 IMP
    positive regulation of gene expression GO:0010628 IMP
Subcellular Localization
    type III intermediate filament GO:0045098 TAS
    cell projection GO:0042995 ISO
    peroxisome GO:0005777 ISO
    intermediate filament cytoskeleton GO:0045111 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
    cell body GO:0044297 ISO
    axon GO:0030424 ISO
    cytoplasm GO:0005737 ISO
    intermediate filament GO:0005882 ISO
    focal adhesion GO:0005925 ISO
    neuron projection GO:0043005 IDA
    cytoskeleton GO:0005856 ISO
    plasma membrane GO:0005886 ISO
    cytosol GO:0005829 ISO
    extracellular exosome GO:0070062 ISO
    cell leading edge GO:0031252 IDA
 Experiment description of studies that identified Vim in exosomes
1
Experiment ID 210
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD81|FLOT1
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 39
ISEV standards
✔
IEM
EV Biophysical techniques
✔
TSG101|HSC70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
3
Experiment ID 263
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-γ via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th1 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Vim
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Vim is involved
PathwayEvidenceSource
Caspase-mediated cleavage of cytoskeletal proteins IEA Reactome
Striated Muscle Contraction IEA Reactome





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