Gene description for CLASP2
Gene name cytoplasmic linker associated protein 2
Gene symbol CLASP2
Other names/aliases -
Species Homo sapiens
 Database cross references - CLASP2
ExoCarta ExoCarta_23122
Vesiclepedia VP_23122
Entrez Gene 23122
HGNC 17078
MIM 605853
UniProt O75122  
 CLASP2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells Unpublished / Not applicable
Mesenchymal stem cells 36408942    
 Gene ontology annotations for CLASP2
Molecular Function
    dystroglycan binding GO:0002162 IPI
    protein binding GO:0005515 IPI
    microtubule binding GO:0008017 IBA
    microtubule binding GO:0008017 IDA
    microtubule plus-end binding GO:0051010 IDA
    actin filament binding GO:0051015 IDA
    protein tyrosine kinase binding GO:1990782 IPI
Biological Process
    microtubule cytoskeleton organization GO:0000226 IMP
    vesicle targeting GO:0006903 IMP
    microtubule nucleation GO:0007020 IMP
    negative regulation of microtubule depolymerization GO:0007026 NAS
    Golgi organization GO:0007030 IMP
    mitotic spindle organization GO:0007052 IMP
    establishment or maintenance of cell polarity GO:0007163 TAS
    exit from mitosis GO:0010458 IMP
    regulation of gastrulation GO:0010470 IMP
    positive regulation of epithelial cell migration GO:0010634 IMP
    regulation of epithelial to mesenchymal transition GO:0010717 IMP
    regulation of axon extension GO:0030516 IDA
    microtubule organizing center organization GO:0031023 IMP
    regulation of microtubule polymerization or depolymerization GO:0031110 IMP
    regulation of microtubule polymerization GO:0031113 IDA
    regulation of microtubule-based process GO:0032886 IMP
    regulation of actin cytoskeleton organization GO:0032956 IDA
    microtubule anchoring GO:0034453 IMP
    platelet-derived growth factor receptor-beta signaling pathway GO:0035791 IDA
    establishment of mitotic spindle localization GO:0040001 IBA
    positive regulation of exocytosis GO:0045921 IMP
    cell division GO:0051301 IEA
    negative regulation of stress fiber assembly GO:0051497 IMP
    negative regulation of focal adhesion assembly GO:0051895 IMP
    protein localization to plasma membrane GO:0072659 IMP
    positive regulation of extracellular matrix disassembly GO:0090091 IMP
    mitotic spindle assembly GO:0090307 IBA
    presynaptic cytoskeleton organization GO:0099187 IDA
    presynaptic cytoskeleton organization GO:0099187 IMP
    negative regulation of wound healing, spreading of epidermal cells GO:1903690 IMP
    positive regulation of basement membrane assembly involved in embryonic body morphogenesis GO:1904261 IMP
Subcellular Localization
    kinetochore GO:0000776 IMP
    cytoplasm GO:0005737 NAS
    Golgi apparatus GO:0005794 IDA
    trans-Golgi network GO:0005802 IDA
    centrosome GO:0005813 IEA
    microtubule organizing center GO:0005815 IBA
    kinetochore microtubule GO:0005828 TAS
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    microtubule GO:0005874 IDA
    microtubule GO:0005874 IDA
    spindle microtubule GO:0005876 IBA
    cytoplasmic microtubule GO:0005881 IBA
    cytoplasmic microtubule GO:0005881 IDA
    plasma membrane GO:0005886 IDA
    focal adhesion GO:0005925 IDA
    cell cortex GO:0005938 IDA
    membrane GO:0016020 HDA
    cell leading edge GO:0031252 IDA
    ruffle membrane GO:0032587 IDA
    axonal growth cone GO:0044295 IDA
    basal cortex GO:0045180 IBA
    basal cortex GO:0045180 IDA
    mitotic spindle GO:0072686 IMP
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
    cortical microtubule plus-end GO:1903754 IDA
 Experiment description of studies that identified CLASP2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
10
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for CLASP2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 LYPD3 27076
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 CSGALNACT2 55454
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 FEZ2 9637
Two-hybrid Homo sapiens
5 CLIP1 6249
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 CENPE 1062
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 DYNC1I2 1781
Affinity Capture-MS Homo sapiens
8 YWHAE 7531
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 ADRB2  
Two-hybrid Homo sapiens
10 FAM43A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 RPL15 6138
Affinity Capture-MS Homo sapiens
12 CSNK2A1 1457
Biochemical Activity Homo sapiens
13 FAXC  
Affinity Capture-MS Homo sapiens
14 APOD 347
Affinity Capture-MS Homo sapiens
15 POR 5447
Affinity Capture-MS Homo sapiens
16 MARK2 2011
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 JAKMIP1 152789
Affinity Capture-MS Homo sapiens
18 UBE2O 63893
Affinity Capture-MS Homo sapiens
19 ACTR1A 10121
Affinity Capture-MS Homo sapiens
20 RCBTB2  
Affinity Capture-MS Homo sapiens
21 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 DNAJB1 3337
Affinity Capture-MS Homo sapiens
23 Cenpe  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
24 PSENEN  
Affinity Capture-MS Homo sapiens
25 PEA15 8682
Affinity Capture-MS Homo sapiens
26 Ssbp1  
Two-hybrid Mus musculus
27 GOPC 57120
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 DYNLT1 6993
Affinity Capture-MS Homo sapiens
29 Mark2 13728
Affinity Capture-MS Mus musculus
30 HYPM  
Affinity Capture-MS Homo sapiens
31 YWHAH 7533
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 ATG16L1 55054
Affinity Capture-MS Homo sapiens
33 Crocc  
Affinity Capture-MS Mus musculus
34 GSK3B 2932
Affinity Capture-MS Homo sapiens
35 TAF5L  
Affinity Capture-MS Homo sapiens
36 YWHAB 7529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 USP12 219333
Affinity Capture-MS Homo sapiens
38 LXN 56925
Affinity Capture-MS Homo sapiens
39 PARK2  
Affinity Capture-MS Homo sapiens
40 STRADA 92335
Affinity Capture-MS Homo sapiens
41 MAPRE3  
Proximity Label-MS Homo sapiens
42 MAPRE1 22919
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 AKR7L 246181
Affinity Capture-MS Homo sapiens
44 GCC2 9648
Affinity Capture-MS Homo sapiens
45 CCR4 1233
Two-hybrid Homo sapiens
46 NTRK1 4914
Affinity Capture-MS Homo sapiens
47 DCAF7 10238
Affinity Capture-MS Homo sapiens
48 PNMA2  
Affinity Capture-MS Homo sapiens
49 DCTN1 1639
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 YWHAQ 10971
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 ANAPC15  
Affinity Capture-MS Homo sapiens
52 PSMA5 5686
Affinity Capture-MS Homo sapiens
53 HIST1H2BK 85236
Cross-Linking-MS (XL-MS) Homo sapiens
54 C10orf76  
Synthetic Lethality Homo sapiens
55 Clip1  
Affinity Capture-Western Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
56 RAD21 5885
Affinity Capture-Western Homo sapiens
57 ID2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 HSPA5 3309
Affinity Capture-MS Homo sapiens
59 CYB5B 80777
Affinity Capture-MS Homo sapiens
60 TP53BP1 7158
Affinity Capture-MS Homo sapiens
61 XPO1 7514
Affinity Capture-MS Homo sapiens
62 RC3H2  
Affinity Capture-MS Homo sapiens
63 CD274 29126
Affinity Capture-MS Homo sapiens
64 YWHAZ 7534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 TUBB4B 10383
Affinity Capture-MS Homo sapiens
66 Soga1  
Affinity Capture-MS Mus musculus
67 NXF1 10482
Affinity Capture-RNA Homo sapiens
68 FASN 2194
Negative Genetic Homo sapiens
69 RPA3 6119
Proximity Label-MS Homo sapiens
70 DYNC1LI1 51143
Affinity Capture-MS Homo sapiens
71 DDRGK1 65992
Affinity Capture-MS Homo sapiens
72 PAFAH1B1 5048
Affinity Capture-MS Homo sapiens
73 MARK3 4140
Affinity Capture-MS Homo sapiens
74 FEZ1  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
75 ASB3 51130
Affinity Capture-MS Homo sapiens
76 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
77 PPP4C 5531
Affinity Capture-MS Homo sapiens
78 GRB2 2885
Affinity Capture-MS Homo sapiens
79 FBXL6  
Affinity Capture-MS Homo sapiens
80 CENPJ 55835
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 DCTN2 10540
Affinity Capture-MS Homo sapiens
82 HIST1H2BC 8347
Cross-Linking-MS (XL-MS) Homo sapiens
83 SMURF1 57154
Affinity Capture-MS Homo sapiens
84 CLIP2 7461
Reconstituted Complex Homo sapiens
85 CREBBP  
Two-hybrid Homo sapiens
86 UBR3  
Affinity Capture-MS Homo sapiens
87 GORASP1 64689
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 DYNC1H1 1778
Affinity Capture-MS Homo sapiens
89 C20orf197  
Affinity Capture-MS Homo sapiens
90 RBFOX2 23543
Two-hybrid Homo sapiens
91 CLASP1 23332
Affinity Capture-MS Homo sapiens
Negative Genetic Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
92 MOV10 4343
Affinity Capture-RNA Homo sapiens
93 Mapre1 13589
Affinity Capture-MS Mus musculus
94 SMYD2 56950
Affinity Capture-MS Homo sapiens
95 WDR48 57599
Affinity Capture-MS Homo sapiens
96 TRIP4 9325
Affinity Capture-MS Homo sapiens
97 KLHDC3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 CIT 11113
Affinity Capture-MS Homo sapiens
99 Cdc6 23834
Affinity Capture-MS Mus musculus
100 CLIP4  
Affinity Capture-MS Homo sapiens
101 RC3H1 149041
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
102 PRMT1 3276
Affinity Capture-MS Homo sapiens
103 NIN 51199
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here