Gene description for STRADA
Gene name STE20-related kinase adaptor alpha
Gene symbol STRADA
Other names/aliases LYK5
NY-BR-96
PMSE
STRAD
Stlk
Species Homo sapiens
 Database cross references - STRADA
ExoCarta ExoCarta_92335
Vesiclepedia VP_92335
Entrez Gene 92335
HGNC 30172
MIM 608626
UniProt Q7RTN6  
 STRADA identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for STRADA
Molecular Function
    protein kinase activity GO:0004672 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    kinase binding GO:0019900 IPI
    protein kinase activator activity GO:0030295 IDA
    protein serine/threonine kinase activator activity GO:0043539 IBA
    protein serine/threonine kinase activator activity GO:0043539 IDA
Biological Process
    protein phosphorylation GO:0006468 IDA
    protein export from nucleus GO:0006611 IBA
    protein export from nucleus GO:0006611 IDA
    activation of protein kinase activity GO:0032147 IDA
    G1 to G0 transition GO:0070314 IPI
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISS
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    intracellular protein-containing complex GO:0140535 IDA
    serine/threonine protein kinase complex GO:1902554 IBA
    serine/threonine protein kinase complex GO:1902554 IPI
    serine/threonine protein kinase complex GO:1902554 ISS
 Experiment description of studies that identified STRADA in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for STRADA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 STK11 6794
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Co-localization Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
2 C5orf24 134553
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 CAB39 51719
Co-crystal Structure Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
4 FBXL19  
Affinity Capture-Western Homo sapiens
5 BIRC6 57448
Affinity Capture-MS Homo sapiens
6 PIP4K2A 5305
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 FASN 2194
Negative Genetic Homo sapiens
8 LBHD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 CAB39L 81617
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 PIP4K2C 79837
Affinity Capture-MS Homo sapiens
11 CLASP2 23122
Affinity Capture-MS Homo sapiens
12 HSP90AA1 3320
Affinity Capture-Western Homo sapiens
13 SHMT2 6472
Affinity Capture-RNA Homo sapiens
14 XPO1 7514
Affinity Capture-MS Homo sapiens
15 DDX58 23586
Affinity Capture-RNA Homo sapiens
View the network image/svg+xml
 Pathways in which STRADA is involved
PathwayEvidenceSource
Energy dependent regulation of mTOR by LKB1-AMPK TAS Reactome
MTOR signalling TAS Reactome
Signal Transduction TAS Reactome





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