Gene description for BTAF1
Gene name BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, 170kDa
Gene symbol BTAF1
Other names/aliases MOT1
TAF(II)170
TAF172
TAFII170
Species Homo sapiens
 Database cross references - BTAF1
ExoCarta ExoCarta_9044
Vesiclepedia VP_9044
Entrez Gene 9044
HGNC 17307
MIM 605191
UniProt O14981  
 BTAF1 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for BTAF1
Molecular Function
    DNA binding GO:0003677 IEA
    sequence-specific DNA binding transcription factor activity GO:0003700 NAS
    ATP binding GO:0005524 IEA
    helicase activity GO:0004386 IEA
Biological Process
    negative regulation of chromatin binding GO:0035562 IMP
    metabolic process GO:0008152 IEA
    negative regulation of transcription, DNA-templated GO:0045892 NAS
Subcellular Localization
    nucleus GO:0005634 IEA
 Experiment description of studies that identified BTAF1 in exosomes
1
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 217
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|CD81|CD9|CD63
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for BTAF1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MVD 4597
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
2 TBP  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
3 GTF2H4 2968
Co-purification Homo sapiens
View the network image/svg+xml
 Pathways in which BTAF1 is involved
No pathways found





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