Gene description for ANKS1A
Gene name ankyrin repeat and sterile alpha motif domain containing 1A
Gene symbol ANKS1A
Other names/aliases ANKS1
Species Homo sapiens
 Database cross references - ANKS1A
ExoCarta ExoCarta_23294
Vesiclepedia VP_23294
Entrez Gene 23294
HGNC 20961
MIM 608994
UniProt Q92625  
 ANKS1A identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
 Gene ontology annotations for ANKS1A
Molecular Function
    protein binding GO:0005515 IPI
    ephrin receptor binding GO:0046875 IBA
Biological Process
    substrate-dependent cell migration GO:0006929 ISS
    neuron remodeling GO:0016322 ISS
    ephrin receptor signaling pathway GO:0048013 IBA
    ephrin receptor signaling pathway GO:0048013 ISS
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    neuron projection GO:0043005 ISS
 Experiment description of studies that identified ANKS1A in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for ANKS1A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 SFN 2810
Affinity Capture-MS Homo sapiens
3 RNF43  
Proximity Label-MS Homo sapiens
4 NFKBIB  
Affinity Capture-MS Homo sapiens
5 SNX27 81609
Affinity Capture-MS Homo sapiens
6 FASN 2194
Negative Genetic Homo sapiens
7 NPAS1  
Affinity Capture-MS Homo sapiens
8 ERBB2 2064
Protein-peptide Homo sapiens
9 PRKY  
Affinity Capture-MS Homo sapiens
10 BAG3 9531
Affinity Capture-Luminescence Homo sapiens
11 DEF6 50619
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 MARCKS 4082
Proximity Label-MS Homo sapiens
13 YWHAZ 7534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 RIN3  
Affinity Capture-MS Homo sapiens
15 PLBD1 79887
Affinity Capture-MS Homo sapiens
16 YWHAE 7531
Affinity Capture-MS Homo sapiens
17 KCTD14 65987
Affinity Capture-MS Homo sapiens
18 FAM96A  
Affinity Capture-MS Homo sapiens
19 PACSIN2 11252
Co-fractionation Homo sapiens
20 DAB2IP 153090
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 YWHAB 7529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 ERBB4 2066
Protein-peptide Homo sapiens
23 UBC 7316
Reconstituted Complex Homo sapiens
24 EGFR 1956
Protein-peptide Homo sapiens
Two-hybrid Homo sapiens
PCA Homo sapiens
Affinity Capture-Luminescence Homo sapiens
25 CREBBP  
Two-hybrid Homo sapiens
26 SYNGAP1  
Affinity Capture-MS Homo sapiens
27 METTL21B  
Affinity Capture-MS Homo sapiens
28 NPRL2  
Affinity Capture-MS Homo sapiens
29 EPHA8  
Affinity Capture-Western Homo sapiens
30 GSTM3 2947
Affinity Capture-MS Homo sapiens
31 AP2B1 163
Proximity Label-MS Homo sapiens
32 HIF1AN 55662
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 DYNC1LI1 51143
Proximity Label-MS Homo sapiens
34 NT5C 30833
Affinity Capture-MS Homo sapiens
35 PTPN12 5782
Co-fractionation Homo sapiens
36 PSG11  
Affinity Capture-MS Homo sapiens
37 RIN1 9610
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 D2HGDH 728294
Affinity Capture-MS Homo sapiens
39 RFXANK  
Affinity Capture-MS Homo sapiens
40 DNPEP 23549
Co-fractionation Homo sapiens
41 SIRT6  
Affinity Capture-MS Homo sapiens
42 BICD2 23299
Proximity Label-MS Homo sapiens
43 YWHAQ 10971
Affinity Capture-MS Homo sapiens
44 KRAS 3845
Proximity Label-MS Homo sapiens
45 UBXN6 80700
Affinity Capture-MS Homo sapiens
46 RPA1 6117
Co-fractionation Homo sapiens
47 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 ASB6 140459
Affinity Capture-MS Homo sapiens
49 CCT8L2  
Affinity Capture-MS Homo sapiens
50 TRIM47 91107
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which ANKS1A is involved
No pathways found





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