Gene description for USP34
Gene name ubiquitin specific peptidase 34
Gene symbol USP34
Other names/aliases -
Species Homo sapiens
 Database cross references - USP34
ExoCarta ExoCarta_9736
Entrez Gene 9736
HGNC 20066
MIM 615295
UniProt Q70CQ2  
 USP34 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
 Gene ontology annotations for USP34
Molecular Function
    cysteine-type endopeptidase activity GO:0004197 IMP
    ubiquitin-specific protease activity GO:0004843 IDA
    protein binding GO:0005515 IPI
Biological Process
    protein deubiquitination GO:0016579 IDA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IBA
    positive regulation of canonical Wnt signaling pathway GO:0090263 IDA
    protein K48-linked deubiquitination GO:0071108 IDA
    regulation of proteasomal protein catabolic process GO:0061136 IBA
    Wnt signaling pathway GO:0016055 IEA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    nucleus GO:0005634 IBA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified USP34 in exosomes
1
Experiment ID 226
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✔
CD9|FLOT1
EV Membrane markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
✘ EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
 Protein-protein interactions for USP34
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which USP34 is involved
PathwayEvidenceSource
TCF dependent signaling in response to WNT TAS Reactome





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