Gene description for CYBB
Gene name cytochrome b-245, beta polypeptide
Gene symbol CYBB
Other names/aliases AMCBX2
CGD
GP91-1
GP91-PHOX
GP91PHOX
IMD34
NOX2
p91-PHOX
Species Homo sapiens
 Database cross references - CYBB
ExoCarta ExoCarta_1536
Vesiclepedia VP_1536
Entrez Gene 1536
HGNC 2578
MIM 300481
UniProt P04839  
 CYBB identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for CYBB
Molecular Function
    voltage-gated ion channel activity GO:0005244 IEA
    superoxide-generating NADPH oxidase activity GO:0016175 TAS
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
    flavin adenine dinucleotide binding GO:0050660 IMP
    electron carrier activity GO:0009055 IDA
    heme binding GO:0020037 IMP
    protein heterodimerization activity GO:0046982 IPI
Biological Process
    ion transport GO:0006811 IEA
    innate immune response GO:0045087 TAS
    antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent GO:0002479 TAS
    response to nutrient GO:0007584 IEA
    antigen processing and presentation of exogenous peptide antigen via MHC class I GO:0042590 TAS
    hydrogen peroxide biosynthetic process GO:0050665 IEA
    response to drug GO:0042493 IEA
    antigen processing and presentation of peptide antigen via MHC class I GO:0002474 TAS
    phagosome maturation GO:0090382 TAS
    superoxide metabolic process GO:0006801 TAS
    small GTPase mediated signal transduction GO:0007264 TAS
    interaction with host GO:0051701 TAS
    respiratory burst GO:0045730 TAS
    superoxide anion generation GO:0042554 TAS
    inflammatory response GO:0006954 ISS
    vascular endothelial growth factor receptor signaling pathway GO:0048010 TAS
    oxidation-reduction process GO:0055114 TAS
    regulation of ion transmembrane transport GO:0034765 IEA
Subcellular Localization
    neuronal cell body GO:0043025 IEA
    Golgi apparatus GO:0005794 IEA
    mitochondrion GO:0005739 IEA
    NADPH oxidase complex GO:0043020 TAS
    rough endoplasmic reticulum GO:0005791 IEA
    plasma membrane GO:0005886 TAS
    phagocytic vesicle membrane GO:0030670 TAS
    dendrite GO:0030425 IEA
    integral component of plasma membrane GO:0005887 IDA
 Experiment description of studies that identified CYBB in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 217
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|CD81|CD9|CD63
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CYBB
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CYBA 1535
Affinity Capture-MS Homo sapiens
2 NCF2 4688
Affinity Capture-MS Homo sapiens
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