Gene description for CYBB
Gene name cytochrome b-245, beta polypeptide
Gene symbol CYBB
Other names/aliases AMCBX2
CGD
GP91-1
GP91-PHOX
GP91PHOX
IMD34
NOX2
p91-PHOX
Species Homo sapiens
 Database cross references - CYBB
ExoCarta ExoCarta_1536
Vesiclepedia VP_1536
Entrez Gene 1536
HGNC 2578
MIM 300481
UniProt P04839  
 CYBB identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for CYBB
Molecular Function
    protein binding GO:0005515 IPI
    electron transfer activity GO:0009055 IDA
    superoxide-generating NAD(P)H oxidase activity GO:0016175 IDA
    superoxide-generating NAD(P)H oxidase activity GO:0016175 TAS
    heme binding GO:0020037 IMP
    voltage-gated proton channel activity GO:0030171 IDA
    metal ion binding GO:0046872 IEA
    protein heterodimerization activity GO:0046982 IPI
    flavin adenine dinucleotide binding GO:0050660 IMP
    superoxide-generating NADPH oxidase activity GO:0106292 IMP
Biological Process
    superoxide metabolic process GO:0006801 IDA
    superoxide metabolic process GO:0006801 TAS
    defense response GO:0006952 IBA
    inflammatory response GO:0006954 ISS
    response to nutrient GO:0007584 IEA
    response to xenobiotic stimulus GO:0009410 IEA
    positive regulation of tumor necrosis factor production GO:0032760 IEA
    monoatomic ion transmembrane transport GO:0034220 IEA
    superoxide anion generation GO:0042554 IBA
    superoxide anion generation GO:0042554 IDA
    superoxide anion generation GO:0042554 TAS
    innate immune response GO:0045087 IMP
    innate immune response GO:0045087 ISS
    innate immune response GO:0045087 TAS
    respiratory burst GO:0045730 IMP
    respiratory burst GO:0045730 NAS
    respiratory burst GO:0045730 TAS
    positive regulation of angiogenesis GO:0045766 IEA
    hydrogen peroxide biosynthetic process GO:0050665 IEA
    cellular response to cadmium ion GO:0071276 IEA
    cellular response to ethanol GO:0071361 IEA
    hypoxia-inducible factor-1alpha signaling pathway GO:0097411 IEA
    response to aldosterone GO:1904044 IEA
    cellular response to L-glutamine GO:1904845 IEA
    response to angiotensin GO:1990776 IEA
Subcellular Localization
    nuclear envelope GO:0005635 IEA
    endoplasmic reticulum membrane GO:0005789 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 NAS
    plasma membrane GO:0005886 TAS
    dendrite GO:0030425 IEA
    phagocytic vesicle membrane GO:0030670 TAS
    monoatomic ion channel complex GO:0034702 IEA
    specific granule membrane GO:0035579 TAS
    NADPH oxidase complex GO:0043020 IBA
    NADPH oxidase complex GO:0043020 IDA
    NADPH oxidase complex GO:0043020 NAS
    NADPH oxidase complex GO:0043020 TAS
    neuronal cell body GO:0043025 IEA
    tertiary granule membrane GO:0070821 TAS
    perinuclear endoplasmic reticulum GO:0097038 IEA
 Experiment description of studies that identified CYBB in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CYBB
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CYBA 1535
Affinity Capture-MS Homo sapiens
2 FBXW7  
Synthetic Lethality Homo sapiens
3 IQGAP1 8826
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
4 ACTB 60
Affinity Capture-Western Homo sapiens
5 GNG8  
Affinity Capture-MS Homo sapiens
6 SLC30A1 7779
Affinity Capture-MS Homo sapiens
7 CCDC168  
Affinity Capture-MS Homo sapiens
8 KLK10 5655
Affinity Capture-MS Homo sapiens
9 C17orf62 79415
Two-hybrid Homo sapiens
10 ARL10  
Affinity Capture-MS Homo sapiens
11 USP34 9736
Affinity Capture-MS Homo sapiens
12 LTB4R 1241
Affinity Capture-Western Homo sapiens
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