Gene description for DNAJB2
Gene name DnaJ (Hsp40) homolog, subfamily B, member 2
Gene symbol DNAJB2
Other names/aliases CMT2T
DSMA5
HSJ-1
HSJ1
HSPF3
Species Homo sapiens
 Database cross references - DNAJB2
ExoCarta ExoCarta_3300
Vesiclepedia VP_3300
Entrez Gene 3300
HGNC 5228
MIM 604139
UniProt P25686  
 DNAJB2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for DNAJB2
Molecular Function
    ATPase activator activity GO:0001671 IBA
    ATPase activator activity GO:0001671 IDA
    protein binding GO:0005515 IPI
    Hsp70 protein binding GO:0030544 IBA
    Hsp70 protein binding GO:0030544 IDA
    Hsp70 protein binding GO:0030544 IPI
    polyubiquitin modification-dependent protein binding GO:0031593 IDA
    ubiquitin protein ligase binding GO:0031625 IPI
    ubiquitin binding GO:0043130 IDA
    unfolded protein binding GO:0051082 IBA
    unfolded protein binding GO:0051082 IDA
    protein-folding chaperone binding GO:0051087 IPI
    proteasome binding GO:0070628 IDA
    protein serine/threonine kinase binding GO:0120283 IPI
    ubiquitin-modified protein reader activity GO:0140036 IDA
    protein transporter activity GO:0140318 TAS
Biological Process
    response to unfolded protein GO:0006986 TAS
    negative regulation of cell population proliferation GO:0008285 IGI
    negative regulation of cell growth GO:0030308 IGI
    regulation of protein ubiquitination GO:0031396 IMP
    positive regulation of protein ubiquitination GO:0031398 IDA
    negative regulation of protein binding GO:0032091 IDA
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 IDA
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 TAS
    positive regulation of ATP-dependent activity GO:0032781 IDA
    regulation of protein localization GO:0032880 IMP
    ERAD pathway GO:0036503 IDA
    protein refolding GO:0042026 IBA
    protein refolding GO:0042026 IDA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IMP
    chaperone-mediated protein folding GO:0061077 IBA
    chaperone-mediated protein folding GO:0061077 IMP
    neuron cellular homeostasis GO:0070050 TAS
    negative regulation of inclusion body assembly GO:0090084 IDA
    negative regulation of inclusion body assembly GO:0090084 IMP
    negative regulation of protein deubiquitination GO:0090086 IDA
    regulation of chaperone-mediated protein folding GO:1903644 IDA
Subcellular Localization
    proteasome complex GO:0000502 IDA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
    endoplasmic reticulum membrane GO:0005789 IDA
    endoplasmic reticulum membrane GO:0005789 IEA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    inclusion body GO:0016234 IDA
    nuclear membrane GO:0031965 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    cytoplasmic side of endoplasmic reticulum membrane GO:0098554 TAS
 Experiment description of studies that identified DNAJB2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for DNAJB2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PSMA6 5687
Affinity Capture-Western Homo sapiens
2 Hsp90ab1 15516
Affinity Capture-Western Mus musculus
3 ISG15 9636
Affinity Capture-MS Homo sapiens
4 SPRTN  
Affinity Capture-MS Homo sapiens
5 HSPA6 3310
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 TARDBP 23435
Affinity Capture-Western Homo sapiens
7 BBS7 55212
Proximity Label-MS Homo sapiens
8 GLB1 2720
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 FBXO25  
Biochemical Activity Homo sapiens
10 HSPA1L 3305
Affinity Capture-MS Homo sapiens
11 UBC 7316
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
12 CSNK2A1 1457
Biochemical Activity Homo sapiens
13 SNX6 58533
Proximity Label-MS Homo sapiens
14 DNAJB6 10049
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 TMEM231 79583
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
16 PJA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 HSPA2 3306
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 RHO  
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Luminescence Homo sapiens
19 Hspa1b 15511
Affinity Capture-Western Mus musculus
20 TRO  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 UBQLN1 29979
Reconstituted Complex Homo sapiens
22 SRP14 6727
Proximity Label-MS Homo sapiens
23 STIP1 10963
Proximity Label-MS Homo sapiens
24 RCC1 1104
Proximity Label-MS Homo sapiens
25 SEH1L 81929
Proximity Label-MS Homo sapiens
26 DNAJC7 7266
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
27 TUBA1A 7846
Affinity Capture-MS Homo sapiens
28 ARFGEF2 10564
Affinity Capture-MS Homo sapiens
29 PGAM2 5224
Affinity Capture-MS Homo sapiens
30 DHDH  
Affinity Capture-MS Homo sapiens
31 PRNP 5621
Affinity Capture-MS Homo sapiens
32 BAG5 9529
Affinity Capture-MS Homo sapiens
33 MYO18A 399687
Affinity Capture-MS Homo sapiens
34 MARCH7  
Affinity Capture-MS Homo sapiens
35 DIABLO 56616
Affinity Capture-MS Homo sapiens
36 LACC1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 DNAJB8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 ATP7B 540
Affinity Capture-MS Homo sapiens
39 WRAP73 49856
Affinity Capture-MS Homo sapiens
40 RNF115  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 ASNA1 439
Affinity Capture-MS Homo sapiens
42 CTSA 5476
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 ESRRB  
Affinity Capture-MS Homo sapiens
44 NEK4  
Affinity Capture-MS Homo sapiens
45 Sqstm1  
Affinity Capture-Western Mus musculus
46 ACTB 60
Affinity Capture-MS Homo sapiens
47 PML 5371
Affinity Capture-MS Homo sapiens
48 BBS2 583
Proximity Label-MS Homo sapiens
49 KCTD3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 GET4 51608
Affinity Capture-MS Homo sapiens
51 CYLD  
Affinity Capture-MS Homo sapiens
52 MTR 4548
Proximity Label-MS Homo sapiens
53 BIRC2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 BAG2 9532
Affinity Capture-MS Homo sapiens
55 RLIM 51132
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 SLC25A31 83447
Affinity Capture-MS Homo sapiens
57 CUL3 8452
Affinity Capture-MS Homo sapiens
58 FKBP8 23770
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
59 MAP3K1 4214
Biochemical Activity Homo sapiens
60 MORF4L1  
Affinity Capture-MS Homo sapiens
61 ARL1 400
Affinity Capture-MS Homo sapiens
62 MLF1  
Affinity Capture-MS Homo sapiens
63 KCNE3  
Affinity Capture-MS Homo sapiens
64 HSPA1A 3303
Affinity Capture-MS Homo sapiens
65 HSPA4 3308
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
66 RFWD3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 NSMCE4A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 RNF185  
Affinity Capture-MS Homo sapiens
69 HSPA9 3313
Affinity Capture-MS Homo sapiens
70 ATP7A 538
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 GRB2 2885
Proximity Label-MS Homo sapiens
72 STUB1 10273
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
73 KIAA0100  
Affinity Capture-MS Homo sapiens
74 RPS27A 6233
Affinity Capture-MS Homo sapiens
75 TRAF2 7186
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 Sod1 20655
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
77 USP34 9736
Affinity Capture-MS Homo sapiens
78 RNF19B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 HSPA8 3312
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 SMAD4  
Two-hybrid Homo sapiens
81 Htt  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
82 GRPEL1 80273
Affinity Capture-MS Homo sapiens
83 FAM83H 286077
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 TARS 6897
Proximity Label-MS Homo sapiens
85 Serpinb5  
Affinity Capture-Western Mus musculus
86 TUBB6 84617
Affinity Capture-MS Homo sapiens
87 UBB 7314
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 C10orf88  
Affinity Capture-MS Homo sapiens
89 CYHR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 HSPA4L 22824
Proximity Label-MS Homo sapiens
91 C9orf72  
Affinity Capture-MS Homo sapiens
92 ATXN3 4287
Affinity Capture-Western Homo sapiens
93 HSPA1B 3304
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which DNAJB2 is involved
No pathways found





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