Gene description for UBE4B
Gene name ubiquitination factor E4B
Gene symbol UBE4B
Other names/aliases E4
HDNB1
UBOX3
UFD2
UFD2A
Species Homo sapiens
 Database cross references - UBE4B
ExoCarta ExoCarta_10277
Vesiclepedia VP_10277
Entrez Gene 10277
HGNC 12500
MIM 613565
UniProt O95155  
 UBE4B identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for UBE4B
Molecular Function
    protein binding GO:0005515 IPI
    enzyme binding GO:0019899 ISS
    ubiquitin-ubiquitin ligase activity GO:0034450 IBA
Biological Process
    protein polyubiquitination GO:0000209 IBA
    ubiquitin-dependent protein catabolic process GO:0006511 TAS
    granzyme-mediated apoptotic signaling pathway GO:0008626 IDA
    response to UV GO:0009411 IDA
    ERAD pathway GO:0036503 IBA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IDA
Subcellular Localization
    ubiquitin ligase complex GO:0000151 TAS
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISS
 Experiment description of studies that identified UBE4B in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
8
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
9
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
10
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for UBE4B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 XPC  
Affinity Capture-Western Homo sapiens
2 VPS35 55737
Co-fractionation Homo sapiens
3 PPP3CA 5530
Biochemical Activity Homo sapiens
4 PPP2R4 5524
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
5 MAPK3 5595
Biochemical Activity Homo sapiens
6 SRPRB 58477
Co-fractionation Homo sapiens
7 ZPR1 8882
Co-fractionation Homo sapiens
8 NUDCD3 23386
Co-fractionation Homo sapiens
9 HRAS 3265
Reconstituted Complex Homo sapiens
10 USP9X 8239
Co-fractionation Homo sapiens
11 LRRC73  
Affinity Capture-MS Homo sapiens
12 RBM12 10137
Co-fractionation Homo sapiens
13 SBDS 51119
Co-fractionation Homo sapiens
14 VPS29 51699
Co-fractionation Homo sapiens
15 VPS26A 9559
Co-fractionation Homo sapiens
16 PCID2 55795
Co-fractionation Homo sapiens
17 NDUFA7 4701
Co-fractionation Homo sapiens
18 PRC1 9055
Affinity Capture-MS Homo sapiens
19 HGS 9146
Reconstituted Complex Homo sapiens
20 UBQLN1 29979
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
21 SARNP 84324
Co-fractionation Homo sapiens
22 NUSAP1 51203
Co-fractionation Homo sapiens
23 BTRC 8945
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
24 RAD23A 5886
Reconstituted Complex Homo sapiens
25 PDS5B 23047
Co-fractionation Homo sapiens
26 UBE2V1 7335
Co-fractionation Homo sapiens
27 RAD23B 5887
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 UBE4B 10277
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
29 TTC4  
Co-fractionation Homo sapiens
30 CTDP1 9150
Affinity Capture-MS Homo sapiens
31 PGAM5 192111
Biochemical Activity Homo sapiens
32 TMEM165 55858
Synthetic Lethality Homo sapiens
33 STAU1 6780
Co-fractionation Homo sapiens
34 CBL 867
Co-crystal Structure Homo sapiens
35 PSMB1 5689
Co-fractionation Homo sapiens
36 UBXN1 51035
Affinity Capture-MS Homo sapiens
37 RPA2 6118
Co-fractionation Homo sapiens
38 VCP 7415
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
39 NTRK1 4914
Affinity Capture-MS Homo sapiens
40 EYA3  
Affinity Capture-MS Homo sapiens
41 TTC9C 283237
Co-fractionation Homo sapiens
42 UBE2D3 7323
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
43 VAV2 7410
Two-hybrid Homo sapiens
44 CDC27 996
Affinity Capture-Western Homo sapiens
45 NLRP10  
Affinity Capture-MS Homo sapiens
46 STAM 8027
Affinity Capture-Western Homo sapiens
47 IGBP1 3476
Co-fractionation Homo sapiens
48 PCDHA8  
Affinity Capture-MS Homo sapiens
49 NUMA1 4926
Co-fractionation Homo sapiens
50 VPS4B 9525
Co-fractionation Homo sapiens
51 AHCYL1 10768
Co-fractionation Homo sapiens
52 UBXN7 26043
Co-fractionation Homo sapiens
53 UBE2L3 7332
Co-fractionation Homo sapiens
54 ZC3H8  
Co-fractionation Homo sapiens
55 DUSP16  
Affinity Capture-MS Homo sapiens
56 ZFYVE19 84936
Co-fractionation Homo sapiens
57 Cep152  
Affinity Capture-MS Mus musculus
58 UBE2D2 7322
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
59 RPA3 6119
Proximity Label-MS Homo sapiens
60 AMZ1  
Affinity Capture-MS Homo sapiens
61 DLST 1743
Co-fractionation Homo sapiens
62 TP63  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
63 UBL7  
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
64 HDLBP 3069
Co-fractionation Homo sapiens
65 FAM114A2  
Co-fractionation Homo sapiens
66 FOXK1 221937
Co-fractionation Homo sapiens
67 TP73  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
68 THY1 7070
Co-fractionation Homo sapiens
69 PDE3A  
Co-fractionation Homo sapiens
70 MDM2  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
71 PPIF 10105
Co-fractionation Homo sapiens
72 UBE2D1 7321
Co-fractionation Homo sapiens
73 STUB1 10273
Co-fractionation Homo sapiens
74 VPS36 51028
Co-fractionation Homo sapiens
75 CDC25A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 TRIP6 7205
Co-fractionation Homo sapiens
77 FEZ1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
78 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
79 FBXW11  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
80 ATXN3 4287
Reconstituted Complex Homo sapiens
81 ZRANB2 9406
Co-fractionation Homo sapiens
82 TP53 7157
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
83 USP34 9736
Co-fractionation Homo sapiens
84 SULT1A1 6817
Co-fractionation Homo sapiens
85 SF3B4 10262
Co-fractionation Homo sapiens
86 MRPL53  
Co-fractionation Homo sapiens
87 MEN1 4221
Affinity Capture-MS Homo sapiens
88 EDEM1  
Affinity Capture-MS Homo sapiens
89 CAPZA2 830
Co-fractionation Homo sapiens
90 UBE2C 11065
Co-fractionation Homo sapiens
91 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
92 TTK 7272
Affinity Capture-Western Homo sapiens
93 OTUB1 55611
Biochemical Activity Homo sapiens
94 UCHL3 7347
Co-fractionation Homo sapiens
95 ATP6V1F 9296
Co-fractionation Homo sapiens
96 TRIP12 9320
Reconstituted Complex Homo sapiens
97 NDUFA2 4695
Co-fractionation Homo sapiens
98 OR7A5  
Affinity Capture-MS Homo sapiens
99 PINK1  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
100 SSSCA1 10534
Co-fractionation Homo sapiens
101 LDLR 3949
Positive Genetic Homo sapiens
102 RPA1 6117
Co-fractionation Homo sapiens
103 UBA6 55236
Co-fractionation Homo sapiens
104 PRMT1 3276
Biochemical Activity Homo sapiens
105 UFM1 51569
Co-fractionation Homo sapiens
106 CDKN2A 1029
Reconstituted Complex Homo sapiens
107 SCO2  
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which UBE4B is involved
No pathways found





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