Gene description for SF3B4
Gene name splicing factor 3b, subunit 4, 49kDa
Gene symbol SF3B4
Other names/aliases AFD1
Species Homo sapiens
 Database cross references - SF3B4
ExoCarta ExoCarta_10262
Entrez Gene 10262
HGNC 10771
MIM 605593
UniProt Q15427  
 SF3B4 identified in exosomes derived from the following tissue/cell type
Squamous carcinoma cells 20124223    
 Gene ontology annotations for SF3B4
Molecular Function
    nucleotide binding GO:0000166 IEA
    poly(A) RNA binding GO:0044822 IDA
    protein binding GO:0005515 IPI
Biological Process
    RNA splicing, via transesterification reactions GO:0000375 TAS
    RNA splicing GO:0008380 TAS
    gene expression GO:0010467 TAS
    positive regulation of mRNA splicing, via spliceosome GO:0048026 IEA
    mRNA processing GO:0006397 TAS
    mRNA splicing, via spliceosome GO:0000398 TAS
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    spliceosomal complex GO:0005681 IDA
    U12-type spliceosomal complex GO:0005689 IDA
 Experiment description of studies that identified SF3B4 in exosomes
Experiment ID 191
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SF3B4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NCK2 8440
Two-hybrid Homo sapiens
2 RNU11 26824
Affinity Capture-MS Homo sapiens
3 SF3B6 51639
Invitro Homo sapiens
4 TCERG1  
Affinity Capture-MS Homo sapiens
5 SF3B2 10992
Invivo Homo sapiens
Two-hybrid Homo sapiens
6 SMNDC1  
Affinity Capture-MS Homo sapiens
7 BMPR1A  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
8 SS18L1  
Two-hybrid Homo sapiens
9 MAPK9 5601
Two-hybrid Homo sapiens
10 TCAF1 9747
Two-hybrid Homo sapiens
11 RNU12  
Affinity Capture-MS Homo sapiens
12 PHF5A 84844
Invivo Homo sapiens
View the network image/svg+xml
 Pathways in which SF3B4 is involved
mRNA Splicing - Major Pathway TAS Reactome
mRNA Splicing - Minor Pathway TAS Reactome

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