Gene description for NUB1
Gene name negative regulator of ubiquitin-like proteins 1
Gene symbol NUB1
Other names/aliases BS4
NUB1L
NYREN18
Species Homo sapiens
 Database cross references - NUB1
ExoCarta ExoCarta_51667
Vesiclepedia VP_51667
Entrez Gene 51667
HGNC 17623
MIM 607981
UniProt Q9Y5A7  
 NUB1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for NUB1
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    ubiquitin-dependent protein catabolic process GO:0006511 IMP
    protein ubiquitination GO:0016567 IMP
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 IMP
    response to type II interferon GO:0034341 IEP
    response to tumor necrosis factor GO:0034612 IEP
    regulation of ubiquitin-dependent protein catabolic process GO:2000058 IBA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    nucleolus GO:0005730 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified NUB1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NUB1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CCDC12  
Affinity Capture-MS Homo sapiens
2 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
3 UFD1L 7353
Reconstituted Complex Homo sapiens
4 LCN8  
Affinity Capture-MS Homo sapiens
5 AIPL1  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
6 GSK3B 2932
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
7 TP53 7157
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
8 ECT2 1894
Affinity Capture-MS Homo sapiens
9 FAT1 2195
Affinity Capture-Western Homo sapiens
10 SF3B4 10262
Two-hybrid Homo sapiens
11 VAV1 7409
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 UBC 7316
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
13 PSMB4 5692
Affinity Capture-Western Homo sapiens
14 SNCAIP  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
15 SHFM1 7979
Affinity Capture-MS Homo sapiens
16 PSMD4 5710
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
17 UBD  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
18 SOX2  
Affinity Capture-MS Homo sapiens
19 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
20 COPB1 1315
Two-hybrid Homo sapiens
21 UBB 7314
Affinity Capture-MS Homo sapiens
22 FANCA  
Two-hybrid Homo sapiens
23 TERF1 7013
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
24 MAPT  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
25 RPA3 6119
Proximity Label-MS Homo sapiens
26 VCP 7415
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
27 RNF168  
Affinity Capture-MS Homo sapiens
28 UBE2K 3093
Two-hybrid Homo sapiens
29 NEDD8 4738
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
30 HSPA4 3308
Co-fractionation Homo sapiens
31 PSMC5 5705
Affinity Capture-MS Homo sapiens
32 C9orf72  
Affinity Capture-MS Homo sapiens
33 SEMA4C 54910
Affinity Capture-MS Homo sapiens
34 PSMD2 5708
Reconstituted Complex Homo sapiens
35 CUL4A 8451
Affinity Capture-MS Homo sapiens
36 EP300 2033
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which NUB1 is involved
PathwayEvidenceSource
Metabolism of proteins TAS Reactome
Neddylation TAS Reactome
Post-translational protein modification TAS Reactome





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