Gene description for SF3B6
Gene name splicing factor 3b, subunit 6, 14kDa
Gene symbol SF3B6
Other names/aliases CGI-110
HSPC175
Ht006
P14
SAP14
SAP14a
SF3B14
SF3B14a
Species Homo sapiens
 Database cross references - SF3B6
ExoCarta ExoCarta_51639
Vesiclepedia VP_51639
Entrez Gene 51639
HGNC 30096
MIM 607835
UniProt Q9Y3B4  
 SF3B6 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 34887515    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Thymus 23844026    
 Gene ontology annotations for SF3B6
Molecular Function
    RNA binding GO:0003723 HDA
    mRNA binding GO:0003729 IDA
    protein binding GO:0005515 IPI
Biological Process
    mRNA splicing, via spliceosome GO:0000398 IDA
    mRNA splicing, via spliceosome GO:0000398 NAS
    blastocyst formation GO:0001825 IEA
    U2-type prespliceosome assembly GO:1903241 NAS
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleus GO:0005634 NAS
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    spliceosomal complex GO:0005681 NAS
    U2-type spliceosomal complex GO:0005684 IDA
    U2-type spliceosomal complex GO:0005684 IPI
    U2 snRNP GO:0005686 NAS
    U12-type spliceosomal complex GO:0005689 IDA
 Experiment description of studies that identified SF3B6 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
6
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SF3B6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 P4HA1 5033
Affinity Capture-MS Homo sapiens
3 SNRPG 6637
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 SNRPE 6635
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 DNAJC8 22826
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 PRPF8 10594
Co-fractionation Homo sapiens
7 SCNM1  
Affinity Capture-MS Homo sapiens
8 ARHGAP26 23092
Affinity Capture-MS Homo sapiens
9 RPA2 6118
Proximity Label-MS Homo sapiens
10 USP39 10713
Co-fractionation Homo sapiens
11 SRSF11 9295
Co-fractionation Homo sapiens
12 LAMP2 3920
Co-fractionation Homo sapiens
13 CAND1 55832
Affinity Capture-MS Homo sapiens
14 SNRPF 6636
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 SNRPD2 6633
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
16 APP 351
Reconstituted Complex Homo sapiens
17 SF3B5 83443
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 KIAA1429 25962
Affinity Capture-MS Homo sapiens
19 SRP54 6729
Co-fractionation Homo sapiens
20 XRCC1 7515
Affinity Capture-MS Homo sapiens
21 KIF23 9493
Affinity Capture-MS Homo sapiens
22 SPRTN  
Affinity Capture-MS Homo sapiens
23 SF3B1 23451
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 PUF60 22827
Co-fractionation Homo sapiens
25 AGO3  
Affinity Capture-MS Homo sapiens
26 SNRPC 6631
Affinity Capture-MS Homo sapiens
27 SAFB 6294
Co-fractionation Homo sapiens
28 SF3A2 8175
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 SNRNP48  
Affinity Capture-MS Homo sapiens
30 Plekhg5  
Affinity Capture-MS Mus musculus
31 PPP4R2  
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 RCC1 1104
Co-fractionation Homo sapiens
33 ITGA4 3676
Affinity Capture-MS Homo sapiens
34 EED  
Affinity Capture-MS Homo sapiens
35 U2AF1 7307
Co-fractionation Homo sapiens
36 VCAM1 7412
Affinity Capture-MS Homo sapiens
37 JCHAIN 3512
Affinity Capture-MS Homo sapiens
38 MECP2 4204
Affinity Capture-MS Homo sapiens
39 SLC4A1AP  
Affinity Capture-MS Homo sapiens
40 MYCN  
Affinity Capture-MS Homo sapiens
41 RPS11 6205
Co-fractionation Homo sapiens
42 KIF14 9928
Affinity Capture-MS Homo sapiens
43 ZCRB1  
Affinity Capture-MS Homo sapiens
44 PSME3 10197
Affinity Capture-MS Homo sapiens
45 RBMX 27316
Co-fractionation Homo sapiens
46 VTN 7448
Co-fractionation Homo sapiens
47 CHMP4C 92421
Affinity Capture-MS Homo sapiens
48 SRP9 6726
Affinity Capture-MS Homo sapiens
49 DLD 1738
Affinity Capture-MS Homo sapiens
50 PCNP 57092
Affinity Capture-MS Homo sapiens
51 FOXA1  
Affinity Capture-MS Homo sapiens
52 UTP14A 10813
Co-fractionation Homo sapiens
53 COX15 1355
Affinity Capture-MS Homo sapiens
54 CTDP1 9150
Affinity Capture-MS Homo sapiens
55 CUL4B 8450
Cross-Linking-MS (XL-MS) Homo sapiens
56 RPL4 6124
Two-hybrid Homo sapiens
57 TRA2A 29896
Co-fractionation Homo sapiens
58 SMEK1 55671
Affinity Capture-MS Homo sapiens
59 WDR61 80349
Affinity Capture-MS Homo sapiens
60 TRIM55  
Co-fractionation Homo sapiens
61 SNRPD1 6632
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
62 RAN 5901
Co-fractionation Homo sapiens
63 U2AF2 11338
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
64 TPBG 7162
Co-fractionation Homo sapiens
65 ZRSR2  
Affinity Capture-MS Homo sapiens
66 SON 6651
Co-fractionation Homo sapiens
67 DNM1L 10059
Affinity Capture-MS Homo sapiens
68 CYLD  
Affinity Capture-MS Homo sapiens
69 SF3A3 10946
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 GPATCH11  
Affinity Capture-MS Homo sapiens
71 SNRPB2 6629
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
72 ZNF326 284695
Co-fractionation Homo sapiens
73 ATG16L1 55054
Affinity Capture-MS Homo sapiens
74 SF3B2 10992
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 RBM39 9584
Affinity Capture-MS Homo sapiens
76 SUGP1  
Affinity Capture-MS Homo sapiens
77 FANCD2  
Affinity Capture-MS Homo sapiens
78 KDM4D  
Affinity Capture-MS Homo sapiens
79 LMNB1 4001
Affinity Capture-MS Homo sapiens
80 TPR 7175
Co-fractionation Homo sapiens
81 SNRPB 6628
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
82 HTATSF1 27336
Affinity Capture-MS Homo sapiens
83 SRSF5 6430
Co-fractionation Homo sapiens
84 HSD17B10 3028
Affinity Capture-MS Homo sapiens
85 HNRNPA1 3178
Co-fractionation Homo sapiens
86 SUZ12  
Affinity Capture-MS Homo sapiens
87 SART1 9092
Co-fractionation Homo sapiens
88 FN1 2335
Affinity Capture-MS Homo sapiens
89 SPCS3 60559
Cross-Linking-MS (XL-MS) Homo sapiens
90 SF3A1 10291
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 EFTUD2 9343
Co-fractionation Homo sapiens
92 PRPF19 27339
Co-fractionation Homo sapiens
93 MYC  
Affinity Capture-MS Homo sapiens
94 RPA3 6119
Proximity Label-MS Homo sapiens
95 ZC3H18  
Co-fractionation Homo sapiens
96 DLST 1743
Affinity Capture-MS Homo sapiens
97 TOP1 7150
Co-fractionation Homo sapiens
98 PHF5A 84844
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
99 SNW1 22938
Affinity Capture-MS Homo sapiens
100 SF3B3 23450
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
101 VDAC1 7416
Affinity Capture-MS Homo sapiens
102 HSPD1 3329
Co-fractionation Homo sapiens
103 HNRNPU 3192
Cross-Linking-MS (XL-MS) Homo sapiens
104 FTSJ3 117246
Co-fractionation Homo sapiens
105 PRKDC 5591
Co-fractionation Homo sapiens
106 IFT22 64792
Affinity Capture-MS Homo sapiens
107 U2SURP 23350
Co-fractionation Homo sapiens
108 DHX15 1665
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
109 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
110 PPP4C 5531
Affinity Capture-MS Homo sapiens
111 BCAS2 10286
Co-fractionation Homo sapiens
112 ILF3 3609
Co-fractionation Homo sapiens
113 TTC33  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
114 WWOX 51741
Affinity Capture-MS Homo sapiens
115 SF3B4 10262
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
116 MEN1 4221
Affinity Capture-MS Homo sapiens
117 SNRPA 6626
Affinity Capture-MS Homo sapiens
118 NGEF  
Affinity Capture-MS Homo sapiens
119 SMNDC1  
Co-fractionation Homo sapiens
120 THRAP3 9967
Co-fractionation Homo sapiens
121 Sf3a1 67465
Affinity Capture-MS Mus musculus
122 THOC6 79228
Co-fractionation Homo sapiens
123 CCDC97  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
124 S100A9 6280
Co-fractionation Homo sapiens
125 WDR18 57418
Co-fractionation Homo sapiens
126 RNPC3  
Affinity Capture-MS Homo sapiens
127 UFL1 23376
Affinity Capture-MS Homo sapiens
128 CEBPA  
Protein-peptide Homo sapiens
129 RPS24 6229
Co-fractionation Homo sapiens
130 SRSF7 6432
Co-fractionation Homo sapiens
131 DDX42 11325
Affinity Capture-MS Homo sapiens
132 SOS2 6655
Affinity Capture-MS Homo sapiens
133 CUL7 9820
Affinity Capture-MS Homo sapiens
134 ARHGAP23 57636
Affinity Capture-MS Homo sapiens
135 C9orf72  
Affinity Capture-MS Homo sapiens
136 SNRNP25  
Affinity Capture-MS Homo sapiens
137 ATXN3 4287
Affinity Capture-MS Homo sapiens
138 SNRPA1 6627
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
139 CDC5L 988
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here