Gene description for PEAK1
Gene name pseudopodium-enriched atypical kinase 1
Gene symbol PEAK1
Other names/aliases SGK269
Species Homo sapiens
 Database cross references - PEAK1
ExoCarta ExoCarta_79834
Vesiclepedia VP_79834
Entrez Gene 79834
HGNC 29431
MIM 614248
UniProt Q9H792  
 PEAK1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
 Gene ontology annotations for PEAK1
Molecular Function
    protein kinase activity GO:0004672 IBA
    non-membrane spanning protein tyrosine kinase activity GO:0004715 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IDA
    identical protein binding GO:0042802 IDA
Biological Process
    cell migration GO:0016477 IDA
    cell migration GO:0016477 IMP
    substrate adhesion-dependent cell spreading GO:0034446 IDA
    protein autophosphorylation GO:0046777 IDA
    focal adhesion assembly GO:0048041 IMP
    regulation of focal adhesion assembly GO:0051893 IDA
    regulation of cell motility GO:2000145 IBA
Subcellular Localization
    cytosol GO:0005829 IDA
    focal adhesion GO:0005925 IBA
    focal adhesion GO:0005925 IDA
    actin cytoskeleton GO:0015629 IBA
    actin cytoskeleton GO:0015629 IDA
 Experiment description of studies that identified PEAK1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for PEAK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GJD3  
Proximity Label-MS Homo sapiens
2 PPP1R3A  
Affinity Capture-MS Homo sapiens
3 OCLN 100506658
Proximity Label-MS Homo sapiens
4 SHC1 6464
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 CSK 1445
Affinity Capture-MS Homo sapiens
6 MARCKS 4082
Proximity Label-MS Homo sapiens
7 ATG9A 79065
Proximity Label-MS Homo sapiens
8 GJA1 2697
Proximity Label-MS Homo sapiens
9 BBS7 55212
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 CRK 1398
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 SPSB4  
Affinity Capture-MS Homo sapiens
12 CBWD2  
Affinity Capture-MS Homo sapiens
13 LAMP2 3920
Proximity Label-MS Homo sapiens
14 CALM1 801
Affinity Capture-MS Homo sapiens
15 VWA5A 4013
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 STX4 6810
Proximity Label-MS Homo sapiens
17 PPP1CC 5501
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 CAPZB 832
Affinity Capture-MS Homo sapiens
19 MLLT4 4301
Proximity Label-MS Homo sapiens
20 CARD8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 CALD1 800
Affinity Capture-MS Homo sapiens
22 CALM3 808
Affinity Capture-MS Homo sapiens
23 YWHAG 7532
Affinity Capture-MS Homo sapiens
24 SLC31A1 1317
Affinity Capture-MS Homo sapiens
25 C11orf52 91894
Proximity Label-MS Homo sapiens
26 STX6 10228
Proximity Label-MS Homo sapiens
27 YWHAH 7533
Affinity Capture-MS Homo sapiens
28 RAB35 11021
Proximity Label-MS Homo sapiens
29 ARHGAP39  
Affinity Capture-MS Homo sapiens
30 MAD2L1 4085
Affinity Capture-MS Homo sapiens
31 KIF14 9928
Affinity Capture-MS Homo sapiens
32 MTNR1B  
Two-hybrid Homo sapiens
33 PCDHB11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 PFN1 5216
Proximity Label-MS Homo sapiens
35 AURKA 6790
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 PPP1CA 5499
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 CXADR 1525
Proximity Label-MS Homo sapiens
38 LYN 4067
Proximity Label-MS Homo sapiens
39 MCAM 4162
Proximity Label-MS Homo sapiens
40 PRPS2 5634
Affinity Capture-MS Homo sapiens
41 RAB4A 5867
Proximity Label-MS Homo sapiens
42 DNAJC5 80331
Proximity Label-MS Homo sapiens
43 GADD45A  
Affinity Capture-MS Homo sapiens
44 CDH1 999
Proximity Label-MS Homo sapiens
45 DIRAS3  
Proximity Label-MS Homo sapiens
46 CRKL 1399
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 S100B 6285
Affinity Capture-MS Homo sapiens
48 ACTB 60
Proximity Label-MS Homo sapiens
49 FBLN5 10516
Affinity Capture-MS Homo sapiens
50 C19orf35  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
51 SH2D2A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 SORT1 6272
Affinity Capture-MS Homo sapiens
53 RAB5A 5868
Proximity Label-MS Homo sapiens
54 DQX1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 LAMP1 3916
Proximity Label-MS Homo sapiens
56 CTTN 2017
Affinity Capture-MS Homo sapiens
57 XRCC6BP1  
Affinity Capture-MS Homo sapiens
58 ARF6 382
Proximity Label-MS Homo sapiens
59 MMP13  
Affinity Capture-MS Homo sapiens
60 TGM5  
Affinity Capture-MS Homo sapiens
61 RAB9A 9367
Proximity Label-MS Homo sapiens
62 GOLGA1  
Proximity Label-MS Homo sapiens
63 EBAG9 9166
Proximity Label-MS Homo sapiens
64 RHOB 388
Proximity Label-MS Homo sapiens
65 KRAS 3845
Proximity Label-MS Homo sapiens
66 CTNNA1 1495
Proximity Label-MS Homo sapiens
67 FLOT1 10211
Proximity Label-MS Homo sapiens
68 XRCC5 7520
Affinity Capture-MS Homo sapiens
69 RNF43  
Proximity Label-MS Homo sapiens
70 LAMTOR1 55004
Proximity Label-MS Homo sapiens
71 GRB2 2885
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 EZR 7430
Proximity Label-MS Homo sapiens
73 LCK 3932
Proximity Label-MS Homo sapiens
74 CDH23 64072
Affinity Capture-MS Homo sapiens
75 TOR1AIP1 26092
Affinity Capture-MS Homo sapiens
76 DDX58 23586
Affinity Capture-RNA Homo sapiens
77 AARSD1 80755
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 NCK2 8440
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 ANAPC2 29882
Proximity Label-MS Homo sapiens
80 RAB2A 5862
Proximity Label-MS Homo sapiens
81 PXN 5829
Proximity Label-MS Homo sapiens
82 RAB11A 8766
Proximity Label-MS Homo sapiens
83 EPHA2 1969
Proximity Label-MS Homo sapiens
84 PTPRK 5796
Proximity Label-MS Homo sapiens
85 ANK3  
Proximity Label-MS Homo sapiens
86 CAV1 857
Proximity Label-MS Homo sapiens
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