Gene description for ANAPC1
Gene name anaphase promoting complex subunit 1
Gene symbol ANAPC1
Other names/aliases APC1
MCPR
TSG24
Species Homo sapiens
 Database cross references - ANAPC1
ExoCarta ExoCarta_64682
Entrez Gene 64682
HGNC 19988
MIM 608473
UniProt Q9H1A4  
 ANAPC1 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for ANAPC1
Biological Process
    protein K11-linked ubiquitination GO:0070979 IDA
    anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process GO:0031145 TAS
    regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle GO:0051439 TAS
    negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle GO:0051436 TAS
    mitotic cell cycle GO:0000278 TAS
    mitotic nuclear division GO:0007067 IEA
    mitotic spindle assembly checkpoint GO:0007094 TAS
    cell division GO:0051301 IEA
    positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition GO:0051437 TAS
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    anaphase-promoting complex GO:0005680 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified ANAPC1 in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for ANAPC1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ANAPC4  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
2 FZR1  
Reconstituted Complex Homo sapiens
3 APC2  
Affinity Capture-Western Homo sapiens
4 ANAPC5 51433
Invivo Homo sapiens
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
5 CDC16 8881
Co-purification Homo sapiens
6 CDH1 999
Reconstituted Complex Homo sapiens
7 CDC23 8697
Co-purification Homo sapiens
8 ANAPC11  
Reconstituted Complex Homo sapiens
9 ANAPC7 51434
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
10 CDC27 996
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
11 ANAPC2 29882
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
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