Gene description for TAF4
Gene name TAF4 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 135kDa
Gene symbol TAF4
Other names/aliases TAF2C
TAF2C1
TAF4A
TAFII130
TAFII135
Species Homo sapiens
 Database cross references - TAF4
ExoCarta ExoCarta_6874
Vesiclepedia VP_6874
Entrez Gene 6874
HGNC 11537
MIM 601796
UniProt O00268  
 TAF4 identified in sEVs derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for TAF4
Molecular Function
    core promoter sequence-specific DNA binding GO:0001046 IDA
    DNA binding GO:0003677 IBA
    protein binding GO:0005515 IPI
    RNA polymerase II general transcription initiation factor activity GO:0016251 IBA
    RNA polymerase II general transcription initiation factor activity GO:0016251 IDA
    aryl hydrocarbon receptor binding GO:0017162 IPI
    protein heterodimerization activity GO:0046982 IEA
Biological Process
    ovarian follicle development GO:0001541 IEA
    regulation of DNA repair GO:0006282 NAS
    DNA-templated transcription initiation GO:0006352 IDA
    regulation of transcription by RNA polymerase II GO:0006357 IDA
    transcription by RNA polymerase II GO:0006366 TAS
    transcription initiation at RNA polymerase II promoter GO:0006367 IBA
    transcription initiation at RNA polymerase II promoter GO:0006367 IDA
    mRNA transcription by RNA polymerase II GO:0042789 IDA
    positive regulation of DNA-templated transcription GO:0045893 NAS
    RNA polymerase II preinitiation complex assembly GO:0051123 IPI
    positive regulation of transcription initiation by RNA polymerase II GO:0060261 IDA
Subcellular Localization
    chromatin GO:0000785 IDA
    male germ cell nucleus GO:0001673 IEA
    nucleus GO:0005634 EXP
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    transcription factor TFIID complex GO:0005669 IBA
    transcription factor TFIID complex GO:0005669 IDA
    transcription factor TFIID complex GO:0005669 IPI
    cytosol GO:0005829 IDA
    protein-containing complex GO:0032991 IMP
    transcription factor TFTC complex GO:0033276 IDA
    transcription factor TFTC complex GO:0033276 NAS
    MLL1 complex GO:0071339 IDA
 Experiment description of studies that identified TAF4 in sEVs
1
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for TAF4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 ELF1 1997
Proximity Label-MS Homo sapiens
3 FEV  
Proximity Label-MS Homo sapiens
4 TRRAP 8295
Co-fractionation Homo sapiens
5 ATF7  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
6 TAF1L  
Co-fractionation Homo sapiens
7 SP1  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
8 NFYC 4802
Proximity Label-MS Homo sapiens
9 HIST1H2BH 8345
Cross-Linking-MS (XL-MS) Homo sapiens
10 TAF3 83860
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
11 BRD2  
Affinity Capture-MS Homo sapiens
12 CREM  
Reconstituted Complex Homo sapiens
13 TAF1  
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
14 ELF4 2000
Proximity Label-MS Homo sapiens
15 TAF5  
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
16 YTHDF2 51441
Cross-Linking-MS (XL-MS) Homo sapiens
17 HIST2H2AA3 8337
Cross-Linking-MS (XL-MS) Homo sapiens
18 KIAA1429 25962
Affinity Capture-MS Homo sapiens
19 GTF2A2 2958
Reconstituted Complex Homo sapiens
20 RNPS1 10921
Affinity Capture-MS Homo sapiens
21 TAF9 6880
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
22 GTF2F2 2963
Affinity Capture-Western Homo sapiens
23 KLF8  
Proximity Label-MS Homo sapiens
24 TCF12  
Reconstituted Complex Homo sapiens
25 IMPDH2 3615
Co-fractionation Homo sapiens
26 KIFAP3 22920
Affinity Capture-MS Homo sapiens
27 KLF15  
Proximity Label-MS Homo sapiens
28 CDC27 996
Co-fractionation Homo sapiens
29 ANAPC7 51434
Co-fractionation Homo sapiens
30 ASH2L 9070
Co-fractionation Homo sapiens
31 NAP1L4 4676
Co-fractionation Homo sapiens
32 WDR5 11091
Co-fractionation Homo sapiens
33 KLF16  
Proximity Label-MS Homo sapiens
34 EPB41L3 23136
Affinity Capture-MS Homo sapiens
35 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
36 CDC23 8697
Co-fractionation Homo sapiens
37 TAF10 6881
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
38 SPATA25  
Affinity Capture-MS Homo sapiens
39 PNKP 11284
Affinity Capture-Western Homo sapiens
40 HIST1H2AG 8969
Cross-Linking-MS (XL-MS) Homo sapiens
41 IKZF3  
Affinity Capture-MS Homo sapiens
42 NFIX 4784
Proximity Label-MS Homo sapiens
43 HTT 3064
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
44 HIST1H3E 8353
Affinity Capture-MS Homo sapiens
45 TAF9B  
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
46 YWHAE 7531
Affinity Capture-MS Homo sapiens
47 TRIM21 6737
Affinity Capture-MS Homo sapiens
48 TAF12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Far Western Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
49 CBX5 23468
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
50 IFI16 3428
Affinity Capture-MS Homo sapiens
51 SUPT3H  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
52 CEBPA  
Proximity Label-MS Homo sapiens
53 HIST1H2AB 8335
Cross-Linking-MS (XL-MS) Homo sapiens
54 TAF13  
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
55 TAF7L  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 GTF2A1 2957
Affinity Capture-MS Homo sapiens
57 CBFB 865
Affinity Capture-MS Homo sapiens
58 TAF7  
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
59 ANAPC13 25847
Co-fractionation Homo sapiens
60 TAF15 8148
Co-fractionation Homo sapiens
61 HIST1H3A 8350
Cross-Linking-MS (XL-MS) Homo sapiens
62 SMCHD1 23347
Affinity Capture-MS Homo sapiens
63 CHERP 10523
Affinity Capture-MS Homo sapiens
64 SOX6  
Affinity Capture-MS Homo sapiens
65 ELK3  
Proximity Label-MS Homo sapiens
66 EEF2 1938
Cross-Linking-MS (XL-MS) Homo sapiens
67 HCFC1 3054
Co-fractionation Homo sapiens
68 PHIP 55023
Affinity Capture-MS Homo sapiens
69 EGFR 1956
Affinity Capture-MS Homo sapiens
70 YY1 7528
Proximity Label-MS Homo sapiens
71 FBXO6 26270
Affinity Capture-MS Homo sapiens
72 IKZF1  
Affinity Capture-MS Homo sapiens
73 POLR2A 5430
Affinity Capture-Western Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
74 HIST3H3 8290
Affinity Capture-MS Homo sapiens
Protein-peptide Homo sapiens
75 TBPL1 9519
Affinity Capture-MS Homo sapiens
76 JUN 3725
Reconstituted Complex Homo sapiens
77 HNF4A  
Proximity Label-MS Homo sapiens
78 CREB1  
Affinity Capture-Western Homo sapiens
79 RPA3 6119
Proximity Label-MS Homo sapiens
80 Sept6  
Affinity Capture-MS Mus musculus
81 SF3A1 10291
Affinity Capture-MS Homo sapiens
82 AHR 196
Reconstituted Complex Homo sapiens
83 ETV3  
Proximity Label-MS Homo sapiens
84 SF3B3 23450
Affinity Capture-Western Homo sapiens
85 TRIM28 10155
Co-fractionation Homo sapiens
86 RBBP5 5929
Co-fractionation Homo sapiens
87 KAT2A  
Affinity Capture-Western Homo sapiens
88 BRD3 8019
Affinity Capture-MS Homo sapiens
89 TAF4 6874
Far Western Homo sapiens
Far Western Homo sapiens
90 TAF8  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Far Western Homo sapiens
91 U2SURP 23350
Affinity Capture-MS Homo sapiens
92 DZIP3  
Affinity Capture-MS Homo sapiens
93 TAF6 6878
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
94 RPS27A 6233
Cross-Linking-MS (XL-MS) Homo sapiens
95 FASN 2194
Negative Genetic Homo sapiens
96 ROCK1 6093
Affinity Capture-MS Homo sapiens
97 TP53 7157
Co-fractionation Homo sapiens
98 RUVBL2 10856
Co-fractionation Homo sapiens
99 TBP  
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-RNA Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
100 UBA52 7311
Cross-Linking-MS (XL-MS) Homo sapiens
101 ANAPC1 64682
Co-fractionation Homo sapiens
102 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
103 HIST1H2BD 3017
Cross-Linking-MS (XL-MS) Homo sapiens
104 MAGEA9  
Affinity Capture-MS Homo sapiens
105 DDX58 23586
Affinity Capture-RNA Homo sapiens
106 ESR1  
Affinity Capture-MS Homo sapiens
107 NUP50 10762
Affinity Capture-MS Homo sapiens
108 RUVBL1 8607
Co-fractionation Homo sapiens
109 NCL 4691
Co-fractionation Homo sapiens
110 ZCCHC10  
Affinity Capture-MS Homo sapiens
111 CDC16 8881
Co-fractionation Homo sapiens
112 SF3A3 10946
Affinity Capture-MS Homo sapiens
113 ATXN3 4287
Affinity Capture-Western Homo sapiens
114 FBXO38 81545
Proximity Label-MS Homo sapiens
115 PRMT1 3276
Affinity Capture-MS Homo sapiens
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