Gene description for GTF2A2
Gene name general transcription factor IIA, 2, 12kDa
Gene symbol GTF2A2
Other names/aliases HsT18745
TF2A2
TFIIA
Species Homo sapiens
 Database cross references - GTF2A2
ExoCarta ExoCarta_2958
Vesiclepedia VP_2958
Entrez Gene 2958
HGNC 4647
MIM 600519
UniProt P52657  
 GTF2A2 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for GTF2A2
Molecular Function
    RNA polymerase II general transcription initiation factor binding GO:0001091 IPI
    protein binding GO:0005515 IPI
    RNA polymerase II general transcription initiation factor activity GO:0016251 IBA
    RNA polymerase II general transcription initiation factor activity GO:0016251 IDA
    TBP-class protein binding GO:0017025 IBA
    TBP-class protein binding GO:0017025 IPI
    protein homodimerization activity GO:0042803 IPI
    protein heterodimerization activity GO:0046982 IPI
Biological Process
    transcription by RNA polymerase II GO:0006366 IDA
    transcription initiation at RNA polymerase II promoter GO:0006367 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    RNA polymerase II preinitiation complex assembly GO:0051123 IBA
    RNA polymerase II preinitiation complex assembly GO:0051123 IDA
    RNA polymerase II preinitiation complex assembly GO:0051123 NAS
    positive regulation of transcription initiation by RNA polymerase II GO:0060261 IDA
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    transcription factor TFIID complex GO:0005669 IDA
    transcription factor TFIIA complex GO:0005672 IBA
    transcription factor TFIIA complex GO:0005672 IDA
    transcription factor TFIIA complex GO:0005672 IPI
    cell junction GO:0030054 IDA
 Experiment description of studies that identified GTF2A2 in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for GTF2A2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GTF2A1 2957
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
2 TBP  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
3 KRAS 3845
Negative Genetic Homo sapiens
4 RP2 6102
Affinity Capture-MS Homo sapiens
5 PCBP4 57060
Affinity Capture-MS Homo sapiens
6 TERF1 7013
Affinity Capture-MS Homo sapiens
7 TERF2  
Affinity Capture-MS Homo sapiens
8 TRIM7  
Two-hybrid Homo sapiens
9 CUEDC2  
Affinity Capture-MS Homo sapiens
10 CRNN 49860
Affinity Capture-MS Homo sapiens
11 TBPL1 9519
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 TAF4 6874
Reconstituted Complex Homo sapiens
13 ZG16B 124220
Affinity Capture-MS Homo sapiens
14 S100P 6286
Affinity Capture-MS Homo sapiens
15 RIT1 6016
Negative Genetic Homo sapiens
View the network image/svg+xml



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