Gene description for PCBP4
Gene name poly(rC) binding protein 4
Gene symbol PCBP4
Other names/aliases CBP
LIP4
MCG10
Species Homo sapiens
 Database cross references - PCBP4
ExoCarta ExoCarta_57060
Vesiclepedia VP_57060
Entrez Gene 57060
HGNC 8652
MIM 608503
UniProt P57723  
 PCBP4 identified in sEVs derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for PCBP4
Molecular Function
    DNA binding GO:0003677 IEA
    RNA binding GO:0003723 HDA
    RNA binding GO:0003723 NAS
    mRNA binding GO:0003729 IBA
    mRNA 3'-UTR binding GO:0003730 IDA
    protein binding GO:0005515 IPI
Biological Process
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    regulation of mRNA stability GO:0043488 IDA
    negative regulation of mRNA splicing, via spliceosome GO:0048025 IBA
Subcellular Localization
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    ribonucleoprotein complex GO:1990904 IEA
 Experiment description of studies that identified PCBP4 in sEVs
1
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for PCBP4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TUSC2  
Affinity Capture-MS Homo sapiens
2 SSR3 6747
Affinity Capture-MS Homo sapiens
3 TEX37  
Affinity Capture-MS Homo sapiens
4 KIF14 9928
Affinity Capture-MS Homo sapiens
5 HSPA8 3312
Affinity Capture-MS Homo sapiens
6 ABCB9  
Affinity Capture-MS Homo sapiens
7 SEMA7A  
Co-fractionation Homo sapiens
8 WBP11  
Two-hybrid Homo sapiens
9 GTPBP10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 BAG5 9529
Affinity Capture-MS Homo sapiens
11 PPID 5481
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 QKI 9444
Two-hybrid Homo sapiens
13 LINC01420  
Co-fractionation Homo sapiens
14 TMEM126A 84233
Affinity Capture-MS Homo sapiens
15 RNF138  
Two-hybrid Homo sapiens
16 RBFOX1 54715
Two-hybrid Homo sapiens
17 PSMD14 10213
Affinity Capture-MS Homo sapiens
18 DYNC1H1 1778
Affinity Capture-MS Homo sapiens
19 MIEN1 84299
Affinity Capture-MS Homo sapiens
20 MRPS18C  
Affinity Capture-MS Homo sapiens
21 C8orf22  
Affinity Capture-MS Homo sapiens
22 PCBP1 5093
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
23 CSPG4 1464
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 BAG1 573
Affinity Capture-MS Homo sapiens
25 DYNC1LI2 1783
Affinity Capture-MS Homo sapiens
26 SAV1  
Affinity Capture-MS Homo sapiens
27 CBWD3  
Affinity Capture-MS Homo sapiens
28 PHF5A 84844
Affinity Capture-MS Homo sapiens
29 RLIM 51132
Affinity Capture-MS Homo sapiens
30 HSPA4 3308
Affinity Capture-MS Homo sapiens
31 GTF2A2 2958
Affinity Capture-MS Homo sapiens
32 TRIM11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 ZNF664  
Affinity Capture-MS Homo sapiens
34 MRPL53  
Affinity Capture-MS Homo sapiens
35 LYST 1130
Two-hybrid Homo sapiens
36 TXNDC8 255220
Affinity Capture-MS Homo sapiens
37 VPS4A 27183
Affinity Capture-MS Homo sapiens
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