Gene description for PSMC6
Gene name proteasome (prosome, macropain) 26S subunit, ATPase, 6
Gene symbol PSMC6
Other names/aliases CADP44
HEL-S-73
P44
SUG2
p42
Species Homo sapiens
 Database cross references - PSMC6
ExoCarta ExoCarta_5706
Entrez Gene 5706
HGNC 9553
MIM 602708
UniProt P62333  
 PSMC6 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 19837982    
Keratinocytes 19530224    
Keratinocytes 19530224    
Neuroblastoma cells 25944692    
Prostate cancer cells 25844599    
Thymus 23844026    
Urine 19056867    
 Gene ontology annotations for PSMC6
Molecular Function
    proteasome-activating ATPase activity GO:0036402 IBA
    TBP-class protein binding GO:0017025 IBA
    ATP binding GO:0005524 IEA
    protein binding GO:0005515 IPI
    ATPase activity GO:0016887 TAS
    protein binding, bridging GO:0030674 NAS
Biological Process
    G1/S transition of mitotic cell cycle GO:0000082 TAS
    regulation of cellular amino acid metabolic process GO:0006521 TAS
    regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle GO:0051439 TAS
    ubiquitin-dependent protein catabolic process GO:0006511 IC
    antigen processing and presentation of exogenous peptide antigen via MHC class I GO:0042590 TAS
    positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition GO:0051437 TAS
    gene expression GO:0010467 TAS
    negative regulation of apoptotic process GO:0043066 TAS
    positive regulation of canonical Wnt signaling pathway GO:0090263 TAS
    ER-associated ubiquitin-dependent protein catabolic process GO:0030433 IBA
    negative regulation of canonical Wnt signaling pathway GO:0090090 TAS
    DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest GO:0006977 TAS
    innate immune response GO:0045087 TAS
    regulation of apoptotic process GO:0042981 TAS
    positive regulation of RNA polymerase II transcriptional preinitiation complex assembly GO:0045899 IBA
    small molecule metabolic process GO:0044281 TAS
    negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle GO:0051436 TAS
    apoptotic process GO:0006915 TAS
    antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent GO:0002479 TAS
    stimulatory C-type lectin receptor signaling pathway GO:0002223 TAS
    programmed cell death GO:0012501 TAS
    protein polyubiquitination GO:0000209 TAS
    positive regulation of proteasomal protein catabolic process GO:1901800 IBA
    anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process GO:0031145 TAS
    mitotic cell cycle GO:0000278 TAS
    cellular nitrogen compound metabolic process GO:0034641 TAS
    viral process GO:0016032 TAS
    antigen processing and presentation of peptide antigen via MHC class I GO:0002474 TAS
Subcellular Localization
    proteasome regulatory particle, base subcomplex GO:0008540 IBA
    proteasome accessory complex GO:0022624 ISS
    proteasome complex GO:0000502 IDA
    cytosolic proteasome complex GO:0031597 IBA
    cytosol GO:0005829 TAS
    membrane GO:0016020 IDA
    extracellular exosome GO:0070062 IDA
    nuclear proteasome complex GO:0031595 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified PSMC6 in exosomes
1
Experiment ID 21
ISEV standards
EM|IEM
EV Biophysical techniques
Alix|TSG101|HSP70
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 189
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19530224    
Organism Homo sapiens
Experiment description Profile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes.
Authors Chavez-Muñoz C, Kilani RT, Ghahary A.
Journal name J Cell Physiol
Publication year 2009
Sample Keratinocytes
Sample name Keratinocytes - Differentiated
Isolation/purification methods Differential centrifugation
Filtration
Sucrose cushion
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 190
ISEV standards
EM
EV Biophysical techniques
HSC70
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19530224    
Organism Homo sapiens
Experiment description Profile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes.
Authors Chavez-Muñoz C, Kilani RT, Ghahary A.
Journal name J Cell Physiol
Publication year 2009
Sample Keratinocytes
Sample name Keratinocytes - Undifferentiated
Isolation/purification methods Differential centrifugation
Filtration
Sucrose cushion
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
5
Experiment ID 275
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|RAB5A
EV Cytosolic markers
CD9|CD82|CD63|CD81
EV Membrane markers
AIF
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
6
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
AQP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for PSMC6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PSMD13 5719
Affinity Capture-MS Homo sapiens
2 DIPA  
Two-hybrid Homo sapiens
3 CRK 1398
Two-hybrid Homo sapiens
4 PSMD9 5715
Two-hybrid Homo sapiens
5 FLJ38984  
Two-hybrid Homo sapiens
6 PSMD6 9861
Affinity Capture-MS Homo sapiens
7 PSMD10 5716
Affinity Capture-MS Homo sapiens
8 WDR71  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PSMC6 is involved
PathwayEvidenceSource
Activation of NF-kappaB in B cells TAS Reactome
Antigen processing: Ubiquitination & Proteasome degradation TAS Reactome
APC/C:Cdc20 mediated degradation of Securin TAS Reactome
APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 TAS Reactome
Asymmetric localization of PCP proteins TAS Reactome
AUF1 (hnRNP D0) destabilizes mRNA TAS Reactome
Autodegradation of Cdh1 by Cdh1:APC/C TAS Reactome
Autodegradation of the E3 ubiquitin ligase COP1 TAS Reactome
Cdc20:Phospho-APC/C mediated degradation of Cyclin A TAS Reactome
CDK-mediated phosphorylation and removal of Cdc6 TAS Reactome
CDK-mediated phosphorylation and removal of Cdc6 TAS Reactome
CDT1 association with the CDC6:ORC:origin complex TAS Reactome
CLEC7A (Dectin-1) signaling TAS Reactome
Cross-presentation of soluble exogenous antigens (endosomes) TAS Reactome
Dectin-1 mediated noncanonical NF-kB signaling TAS Reactome
degradation of AXIN TAS Reactome
Degradation of beta-catenin by the destruction complex TAS Reactome
degradation of DVL TAS Reactome
Degradation of GLI1 by the proteasome TAS Reactome
Degradation of GLI2 by the proteasome TAS Reactome
ER-Phagosome pathway TAS Reactome
GLI3 is processed to GLI3R by the proteasome TAS Reactome
Hedgehog 'on' state TAS Reactome
Hedgehog ligand biogenesis TAS Reactome
Hh mutants that don't undergo autocatalytic processing are degraded by ERAD TAS Reactome
Orc1 removal from chromatin TAS Reactome
Orc1 removal from chromatin TAS Reactome
Regulation of activated PAK-2p34 by proteasome mediated degradation TAS Reactome
Regulation of ornithine decarboxylase (ODC) TAS Reactome
SCF(Skp2)-mediated degradation of p27/p21 TAS Reactome
SCF(Skp2)-mediated degradation of p27/p21 TAS Reactome
SCF-beta-TrCP mediated degradation of Emi1 TAS Reactome
Separation of Sister Chromatids TAS Reactome
Ubiquitin Mediated Degradation of Phosphorylated Cdc25A TAS Reactome
Ubiquitin-dependent degradation of Cyclin D1 TAS Reactome
Vif-mediated degradation of APOBEC3G TAS Reactome
Vpu mediated degradation of CD4 TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here